LOCUS       sequence-135052-       10262 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the GPPS gene plus the lower MVA pathway from 
            Streptococcus pneumoniae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10262)
  TITLE     Expression of the lower MVA pathway in Synechocystis PCC6803
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: GlgA1-Ptrc-GPPS-FNI-MK-PMD-PMK-SmR-psbA2
FEATURES             Location/Qualifiers
     source          1..10262
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(569..574,575..591,592..598)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    607..623
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             5722..6513
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(7371..7389)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7410..7426)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7410..7426)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7423..7445)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7434..7450
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7458..7464,7465..7482,7483..7488))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7503..7524
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7641..7658)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7812..8400)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7892..7911)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8571..9362,9363..9431))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9194..9213
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9432..9536)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9562..10017)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9699..9720)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9911..9930
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10144..10166
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10158..10175
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10159..10175
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10182..10201
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        10182..10200
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     10225..10241
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     10226..10242
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gccatgtccc aaattcttga tcccatcccc aacaaccagc catcagcctt attctgttgc
       61 tacgtcaatg ccaccaatca aatccaagtg gcccgcatta ccaatgtccc taattggtat
      121 tttgaaagag ttgtgttccc tggtcaacgg ttagtatttg aggcagtgcc cagcgctcag
      181 ttagaaattc atactggcat gatggccagc tcgattattt cggacaccat tccctgcgaa
      241 caactgagta ttgatcccga cggattagca gcgggcggtt tcatctctcc agaaaaagaa
      301 cacgagtccg aggatatgac ttcccaatcc ttagtggctt agcaatgaat taatgaattg
      361 gaatacttag gccatgccac cggccggcaa tggatagtcc acggacaaag cactaagaaa
      421 aaggtatagg gatggaaagc agaaactgtt aattactctc tccgatgggt aaccaccacc
      481 gtcatataat tgagcggaaa gtatggcaac caggccctga actcaattag tggaataacg
      541 cggtcctgca ggattctgaa atgagctgtt gacaattaat catccggctc gtataatgtg
      601 tggaaattgt gagcggataa caattaggag gttaattaac aatgacgcgc agcagtaagg
      661 ccttggtcca actagctgat ctatccgaac aagtaaaaaa cgtggtggaa tttgattttg
      721 acaagtatat gcactccaag gccattgcgg ttaatgaggc cttagataaa gttattcccc
      781 cccgctatcc tcaaaaaatc tatgaaagta tgcgctattc cctcctagcc ggcgggaaga
      841 gggttcgacc aattttatgt attgcggcct gtgagctaat gggggggact gaggaacttg
      901 ccatgcctac ggcttgtgcc atcgagatga ttcacactat gagtttgatt catgacgatt
      961 tgccctatat tgataacgat gatttgcgtc gcggtaagcc taccaaccac aaagtttttg
     1021 gtgaagacac ggcgatcatt gctggcgatg cattattgtc attggccttt gaacatgtag
     1081 ccgtgagcac cagtcgtacc ctaggtactg acattatttt acggttgcta tccgaaattg
     1141 gacgcgccac aggaagtgag ggcgtgatgg gtggtcaagt ggtggatatt gaaagcgaag
     1201 gtgatcccag tatagactta gaaacgctgg aatgggtcca tattcataaa acggctgtgt
     1261 tgttggaatg cagtgtcgtg tgtggcgcaa ttatgggggg tgccagcgag gacgacatcg
     1321 agcgtgctag acggtacgct cgctgtgtag gattgctttt ccaagttgtc gatgatattt
     1381 tggatgtaag ccagtcctcg gaagaactcg gaaagactgc tgggaaagat ttgatttctg
     1441 acaaagccac ctatcccaaa ctcatgggtt tggaaaaagc gaaggaattt gccgatgaat
     1501 tactgaaccg tggaaaacag gaacttagtt gttttgatcc taccaaagca gcacctctat
     1561 ttgcgttagc agactacatt gcatctcgtc agaattaaca cattctaacg ggagatacca
     1621 gaacatatga ctaccaatcg caaggatgag cacatcttgt atgcactaga gcaaaaatcc
     1681 agttacaaca gctttgacga agtggaattg atccactcct ccttacccct atacaattta
     1741 gatgaaattg acttgtctac cgagtttgcc ggacgcaaat gggactttcc cttttacatt
     1801 aatgccatga ctgggggcag caacaaagga cgggagatca atcaaaaatt agctcaggtt
     1861 gccgaaactt gtggcatcct atttgttacc ggcagttata gtgcagccct gaaaaatccg
     1921 acggatgatt cctttagtgt gaaaagttcc catccgaatc tcctcttagg tacaaacatt
     1981 ggcctagata aacccgttga actgggcttg caaacagtgg aagaaatgaa ccccgtattg
     2041 ttgcaagtgc atgttaacgt aatgcaggag ctgttgatgc ccgaaggaga acggaaattt
     2101 cgttcctggc agtctcattt agccgactat tccaaacaaa ttcccgtgcc gattgtattg
     2161 aaagaagttg ggtttggcat ggacgccaag acaatcgaac gcgcttatga atttggcgtt
     2221 cgcaccgttg atttgtctgg ccgtggcgga actagctttg cttatattga aaatcggcgg
     2281 agtggccaac gtgactattt gaatcaatgg ggccaatcca ctatgcaggc cttgctgaat
     2341 gcccaagaat ggaaagacaa agtggaatta ctagtgagtg gcggggtgcg taatccctta
     2401 gatatgatta aatgcttagt atttggtgcc aaggcggttg gtctgagtcg taccgtgttg
     2461 gaactggtag aaacctatac tgttgaagaa gtaattggga tcgtccaggg gtggaaggcc
     2521 gacctgcgcc tcatcatgtg ctccttgaac tgtgccacca ttgccgatct gcaaaaagtt
     2581 gattatctgc tgtacggtaa attgaaagaa gcgaacgatc aaatgaaaaa agcgtaggga
     2641 attaggaggt aatatatgac caaaaaagtg ggagtagggc aagcgcactc caagatcatt
     2701 ctaattggcg agcatgcggt cgtctatggc tatcccgcaa tttccctgcc cttgttagag
     2761 gtggaggtga cctgcaaggt cgtgcccgcc gaaagcccgt ggcgcctgta cgaagaagat
     2821 acgctgagca tggcggttta cgcttcccta gaatacttaa atattactga agcttgtatt
     2881 cgttgcgaaa tcgactccgc tattccggaa aaacggggta tggggtccag tgctgcgatt
     2941 tccattgccg caatccgcgc agtgtttgat tattatcaag ctgatctacc acatgacgtg
     3001 ctggaaatcc tcgtgaatcg ggctgaaatg attgctcaca tgaacccgag tggattagac
     3061 gccaaaacct gtctaagtga ccaacccatt cgctttatta agaatgtggg ttttaccgaa
     3121 ttagaaatgg acctcagcgc ctacctggtt attgccgaca ccggagtata tggacatacc
     3181 cgggaagcca ttcaagtggt gcaaaacaaa ggcaaagatg ccctgccctt cttacatgct
     3241 ctaggtgaac tgacccagca agccgaaatt gcaatctccc aaaaagatgc cgaaggcctc
     3301 ggtcaaattt tgagtcaagc ccacttgcac ctaaaagaaa tcggcgtttc ctctctggag
     3361 gctgatagtt tagtggagac cgcactgagt cacggcgctt tgggcgcgaa aatgagcggc
     3421 ggtggcctcg gtggctgcat tattgcgtta gttaccaatt taacgcacgc gcaggaattg
     3481 gcagaacgtt tggaggaaaa gggcgccgtt caaacctgga ttgagtcttt gtagggaatt
     3541 aggaggtaat atatgtatca ttccttgggg aatcaattcg acacccgtac tcggacaagc
     3601 cgcaaaatcc gccgtgaacg ttcctgcagc gacatggacc gtgaacctgt caccgttcgc
     3661 agctacgcta atattgcaat tattaaatat tggggtaaga aaaaagagaa agagatggtt
     3721 ccggcaacca gtagtatctc cctaacattg gaaaatatgt atacagaaac caccttgtct
     3781 ccgctccccg ctaacgtgac cgccgacgag ttttatatta acggccaatt gcaaaatgag
     3841 gtggagcatg ccaagatgtc caagatcatt gatcgctacc gtcctgccgg tgaggggttt
     3901 gtgcgtattg atacacagaa taacatgccc acagcggctg ggctcagttc ttccagctct
     3961 ggtctaagcg ccctggtgaa agcgtgcaat gcctatttta aactcggact ggaccggagt
     4021 cagttagcgc aggaagccaa atttgctagc ggttctagca gtcgtagttt ttacggtccg
     4081 ttaggcgcat gggataaaga ctccggcgaa atttaccccg ttgaaaccga tttaaaattg
     4141 gcaatgatta tgttggtgtt ggaagataag aaaaagccga tcagctcccg cgacggtatg
     4201 aagttatgtg ttgaaacatc cactaccttc gatgattggg tacgccaatc cgaaaaagat
     4261 tatcaggata tgttgattta tttgaaagaa aatgattttg ctaaaattgg ggaactcact
     4321 gaaaagaatg cattggccat gcacgccact accaagacgg cctccccggc ctttagctat
     4381 ttaaccgatg cctcttatga agccatggat tttgtgcggc aattgcgcga aaagggcgaa
     4441 gcgtgttact ttacaatgga cgccgggccg aacgtaaaag tgttctgcca agaaaaagac
     4501 ttagagcact tgagcgaaat ttttggccag cgctaccgct taattgtgag caaaaccaag
     4561 gacttaagcc aggatgactg ttgttaagga attaggaggt aatatatgat cgctgttaaa
     4621 acctgtggta agttgtattg ggccggcgaa tatgccatcc tggaacccgg ccagctggcg
     4681 ttgattaagg acattcccat ttatatgcgg gccgaaattg ccttcagcga ttcttatcgg
     4741 atttatagtg atatgttcga ttttgcggtg gatctccggc cgaatccgga ttatagctta
     4801 atccaagaaa ccatcgcctt aatgggtgac tttctagcag tgcgtggcca gaacctccgc
     4861 cccttttcct tagctattta tggcaaaatg gaacgcgaag ggaagaaatt tggtctaggc
     4921 tcctccggca gtgtggttgt cttggtggtg aaagctttgt tagcgctgta caacttgagc
     4981 gttgatcaaa atctattatt taagttgacc agcgccgtgt tactgaaacg gggcgataac
     5041 ggttccatgg gcgacttagc ttgcatcgca gccgaggact tagtattata ccagagtttt
     5101 gatcgccaaa aagtagctgc ctggttggag gaagagaatt tggccaccgt tctagagcgt
     5161 gactggggct tttctatcag ccaagtaaag ccgactttgg agtgtgattt tctagtgggg
     5221 tggactaaag aggtggctgt aagtagtcac atggtacagc aaattaaaca aaacatcaat
     5281 caaaattttt tgacatccag taaagaaaca gtggtatcct tggtggaagc cctggaacaa
     5341 gggaaatccg agaagattat tgagcaagtt gaagttgctt ctaaactatt agaagggtta
     5401 tccaccgaca tctatacccc tctattgcgg caactaaaag aagccagcca agatttacaa
     5461 gccgttgcga aaagctccgg ggccggcgga ggagattgtg gaattgcctt aagcttcgat
     5521 gctcagagta ctaaaacttt aaagaaccgt tgggccgatt tgggtattga gttgctctac
     5581 caagaacgga ttggacatga cgataaatcc tagggcgcgc ctctagagga tccaggaggt
     5641 aatatatgag ggaagcggtg atcgctgagg atcaccaagg tagtcggcaa ataaggatcc
     5701 tcaggatcca ggaggtaata tatgagggaa gcggtgatcg ccgaagtatc gactcaacta
     5761 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
     5821 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
     5881 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
     5941 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
     6001 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
     6061 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
     6121 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
     6181 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
     6241 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
     6301 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
     6361 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga
     6421 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg
     6481 aaaggcgaga tcaccaaggt agtcggcaaa taaggatcct ccttggtgta atgccaactg
     6541 aataatctgc aaattgcact ctccttcaat ggggggtgct ttttgcttga ctgagtaatc
     6601 ttctgattgc tgatcttgat tgccatcgat cgccggggag tccggggcag ttaccattag
     6661 agagtctaga gaattaatcc atcttcgata gaggaattat gggggaagaa ccctaggcaa
     6721 ttgatggcca tgcgttatgg ctgtatcccc attgtgcggc ggacaggggg tttggtggat
     6781 acggtatcct tctacgatcc tatcaatgaa gccggcaccg gctattgctt tgaccgttat
     6841 gaacccctgg attgctttac ggccatggtg cgggcctggg agggtttccg tttcaaggca
     6901 gattggcaaa aattacagca acgggccatg cgggcagact ttagttggta ccgttccgcc
     6961 ggggaatata tcaaagttta taagggcgtg gtggggaaac cggaggaatt aagccccatg
     7021 gaagaggaaa aaatcgctga gttaactgct tcctatcgct aacaatctcc cggcagtgaa
     7081 gtaaaatcct gaaccctaat cccgctccac tgccgacccc aattctcctt gcctaggcaa
     7141 atttgaaaat tttttctgat caatgcttgt ggtgaagcaa aagctatgtt aacgttataa
     7201 atcgtgccaa tgaagcacaa cgggtcgcta gctcagcggt agagcactcg gcttttaacc
     7261 gattggtctt gggttcgaat cccaggcgac ccattttttc caaaactcca aacatgttac
     7321 caaaagctcc ctaatggtgg tcttgatcgc tcgagctcca gcttttgttc cctttagtga
     7381 gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     7441 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     7501 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     7561 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     7621 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     7681 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     7741 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     7801 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     7861 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     7921 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     7981 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     8041 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     8101 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     8161 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     8221 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     8281 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     8341 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     8401 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     8461 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     8521 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     8581 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     8641 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     8701 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     8761 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     8821 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     8881 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     8941 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     9001 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     9061 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     9121 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     9181 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     9241 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     9301 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     9361 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     9421 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     9481 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     9541 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     9601 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     9661 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     9721 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     9781 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     9841 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     9901 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     9961 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
    10021 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
    10081 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
    10141 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
    10201 gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt gatatcgaat
    10261 tc
//
