LOCUS       sequence-135063-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Modular shuttle vector for Geobacillus and E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Reeve B, Martinez-Klimova E, de Jonghe J, Leak DJ, Ellis T
  TITLE     The Geobacillus Plasmid Set: A Modular Toolkit for Thermophile 
            Engineering.
  JOURNAL   ACS Synth Biol. 2016 Jul 1.
  PUBMED    27332993
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jul 1."
COMMENT     Sequence Label: pG1AK-sfGFP
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..750)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 acgccccttt cggggcgcga aattgagctc ttatcatttg tacagttcat ccataccatg
       61 cgtgatgccc gctgcggtta cgaactccag cagaaccata tgatcgcgtt tctcgttcgg
      121 atctttagac agaacgcttt gcgtgctcag atagtgattg tctggcagca gaacaggacc
      181 atcaccgatt ggagtgtttt gctggtagtg atcagccagc tgcacgctgc catcctccac
      241 gttgtggcga attttaaaat tcgctttaat gccatttttt tgtttatcgg cggtgatgta
      301 aacattgtgg ctgttaaaat tgtattccag cttatggccc aggatattgc cgtcttcttt
      361 aaagtcaatg cctttcagct caatgcggtt taccagggta tcgccttcaa atttcacttc
      421 cgcacgcgtt ttgtacgtgc cgtcatcctt aaaggaaatc gtgcgttcct gcacatagcc
      481 ttccggcatg gcggacttga agaagtcatg ctgcttcata tggtccggat aacgagcaaa
      541 gcactgaaca ccataagtca gcgtcgttac cagagtcggc caaggtaccg gcagtttacc
      601 agtagtacag atgaacttca gcgtcagttt accattagtt gcgtcacctt caccctcgcc
      661 acgcacggaa aacttatgac cgttgacatc accatccagt tccaccagaa tagggacgac
      721 accagtgaac agctcttcgc ctttacgcat tcgaatcact ccttatctag acaatgcttt
      781 ttcatcattg cagcg
//
