LOCUS       sequence-135064-       10473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10473)
  AUTHORS   Zhang P, Medwig-Kinney TN, Goldstein B
  TITLE     Architecture of the cortical actomyosin network driving apical 
            constriction in C. elegans.
  JOURNAL   J Cell Biol. 2023 Sep 4;222(9):e202302102. doi: 
            10.1083/jcb.202302102. Epub 2023 Jun 23.
  PUBMED    37351566
REFERENCE   2  (bases 1 to 10473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-09-4"; volume: "222(9):e202302102. doi"; pages: 
            " 10.1083/jcb.202302102. Epub 2023 Jun 23"
COMMENT     Sequence Label: pDD315 (mTurquoise2^SEC^2xHA)
FEATURES             Location/Qualifiers
     source          1..10473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1914..1934
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    1952..1985
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             1995..2024
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3841..4164)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6082..6106
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6109..7134
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6109..7134
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7135..7159)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    7512..7545
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             7564..7590
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             7591..7617
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(8252..8268)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8252..8268)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8265..8287)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8276..8292
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8300..8306,8307..8324,8325..8330))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8345..8366
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8483..8500)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8654..9242)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8734..8753)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9413..10204,10205..10273))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10036..10055
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10274..10378)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10446..10464
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg gtaagtaagg gcgaagaatt gtttacgggg gtagtaccaa
     1081 tcctcgtcga gctcgacgga gacgtcaacg gacacaagtt ctccgtctcc ggagagggag
     1141 agggagacgc cacctacgga aagctcaccc tcaagttcat ctgcaccacc ggaaagctcc
     1201 cagtcccatg gccaaccctc gtcaccaccc tctcctgggg agtccaatgc ttcgcccgtt
     1261 acccaggtaa gtttgggaat gtatattcaa tgtaatgaag ttggaggatt ttttcagacc
     1321 acatgaagca acacgacttc ttcaagtccg ccatgccaga gggatacgtc caagagcgta
     1381 ccatcttctt caaggacgac ggaaactaca agacccgtgc cgaggtcaag ttcgagggag
     1441 acaccctcgt caaccgtatc gagctcaagg taagtttaac atgggaatcg tgcttctgtt
     1501 taaacaagaa tggttttcag ggaatcgact tcaaggagga cggaaacatc ctcggacaca
     1561 agctcgagta caactacttc tccgacaacg tctacatcac cgccgacaag caaaagaacg
     1621 gaatcaaggc caacttcaag gtaagtttgc attttgtaat aatttgagaa tccaaaaact
     1681 tgctttttca gatccgtcac aacatcgagg acggaggagt ccaactcgcc gaccactacc
     1741 aacaaaacac cccaatcgga gacggaccag tcctcctccc agacaaccac tacctctcca
     1801 cccaatccaa gctctccaag gacccaaacg agaagcgtga ccacatggtc ctcctcgagt
     1861 tcgtcaccgc cgccggaatc accctcggaa tggacgagct ctacaagacc ggtgagaatc
     1921 tgtactttca atccggaaag gtaagtttaa aataacttcg tataatgtat actatacgaa
     1981 gttattttca gggagagcaa aagcttatct ccgaggagga cctctaagga tgatcgacgc
     2041 caacgtcgtt gaattttcaa attttaaata ctgaatattt gttttttttc ctattattta
     2101 tttattctct ttgtgttttt tttcttgctt tctaaaaaat taattcaatc caaatctaaa
     2161 catttttttt tctctttccg tctcccaatt cgtattccgc tcctctcatc tgaacacaat
     2221 gtgcaagttt atttatcttc tcgctttcat ttcattagga cgtgggggga attggtggaa
     2281 gggggaaaca cacaaaagga tgatggaaat gaaataagga cacacaatat gcaacaacat
     2341 tcaattcaga aatatggagg aaggtttaaa agaaaacata aaaatatata gaggaggaag
     2401 gaaactagct gccctcgatt gtctatcttc cattctcacc aagtttcaac aactttattc
     2461 agtcattctt ctctggttca tttcccaacc aatcttcccg aataaagccc atctgtcctt
     2521 tcggccacat tcctcaccta catccgtact ccttatctcc cgttcttatc cagtctttta
     2581 tcgtctctgc gtctcgcaca ctgtctcgtc ggtaatgtaa aaaaaaaaca aaaaaaactt
     2641 gaaaacttgc ggaaagattt cctaataaaa ggagacgaat ccggtaggaa atcgacaagt
     2701 ctctgcttct cgaagactag gtgagttaca tttctttggc cgaattattt tgaaatccta
     2761 aatttgataa tttcagagat gtctgtaaaa cttgcgtgtt atgtgacggc ttcggtcact
     2821 gtcgccactc ttatggtatg cttcatgacc atgtcaacca tctactcgga ggttgatgga
     2881 ttcagagaga agcttgatac cgagatgaat gtgttcagag tgagttaaat tatattttga
     2941 attttaaata atttttaatt ttctagcaat ctaccaatgg attgtggaag gacatagttg
     3001 tcatcggaag atctagcaag cgtgtccgtt gtcaatatga agagaccaac gctaccccaa
     3061 ctccacatgc tgatggatcc ccatctgctc caccaggtca accaccagca gttccaccag
     3121 tcttcaacca gccaaagact ccaaatggag ccaatggaaa tggaccaacc tgcaactgca
     3181 atgctgataa caagtgccca gctggaccat ccggaccaaa gggagttcca ggagttccag
     3241 gactcgacgg agttccagga cttgacggtg ttccaggagt tggagctgat gatatcgctc
     3301 cacaacgcga gtctgtcgga tgcttcactt gcccacaagg accagttgga ccaccaggag
     3361 ctcttggaag accaggacca cgtggacttc caggaccaag aggacaaaat ggaaacccag
     3421 gaagagatgg acaaccagga catccaggag agcaaggatc atccggtcaa atcggaaaga
     3481 tcggagagcc aggaccacca ggagagaagg gacgcgacgc cgagcatcca atcggaagac
     3541 caggaccaaa gggaccaaga ggagatcaag gaccaacagg accagctgga cagaacggtc
     3601 ttcacggacc accaggagag ccaggaaccg ttggaccaga aggaccatct ggaaagcaag
     3661 gacgtcaagg accagacgga acccagggag agactggacc agacggaaga ccaggaaagg
     3721 atgccgagta ctgccagtgc ccagacaagt ctccaccatc agaggctgtc aacgccaacc
     3781 gtggatacag aaatatctaa attgttggtg ttttctaata aaatatttga gattcagtga
     3841 gacttttttc ttggcggcac aataaagttt cctctttttg ttgggtcact caacgaacac
     3901 aatacaaata tttgagcgct cgcatttttc tcaacctttc gggtttaaag cacttcaaaa
     3961 tggaattgaa gagagattgt gaaagtacat cagacagaat cacattttga tagcattcac
     4021 ttcactcaga tgcaaacttt tcagtgacta taaaaagtca taaaataata acattaaaaa
     4081 agcaaataaa ttagcgagag aattttttga caaaaagaaa gaagagtgat agagaagaag
     4141 ggaatgcttg aaaggatttt gcatttatct taatcaccgt cttcgagaag acgaaccatg
     4201 gcgccagttt cagaatccaa gtttctaata tagttcatca caatgttgac gttggtccaa
     4261 cctccggctt gcataatctc tgggatggaa actccagcgc gagccatgtc acgggcagct
     4321 ccgacacggg cggaatgtcc agaccaagcc aagtaacgtt gtccggaatc atccttggct
     4381 ccgtagatga gacggtgggt agcttcgaag attcctgaaa atttattttc agcttattct
     4441 tagctaatat gttgtttaaa cttaccttca agagctcgag tcgagagttg tgatgttgca
     4501 ctcggggcag caactccgtt cttgcgaaca cgacagaaca ggtagttgtt tggatcatcg
     4561 gcgactccgg aaaccgaaat ccagcgctca acgagcttgg taactccaag ggagagcgct
     4621 ttttcgactc cggcagtcga gaccagcgtt tttgtacgtc cgatgtggat gagcattctt
     4681 cctccgtctg tgcgactaat atccttgacg cgaatgcggg caatttcggc gatacggaga
     4741 agtgtgttgt aagcaattcc gaggaaggcg aggtttcgga tgtcctggca acgatcggaa
     4801 ttttccataa gagaacggac ctgatcgaag tcggttctga aaatttcgtc aagtcttagg
     4861 attgaataca ttaatattta aacttacctt tcgaaggcga gggcttgctt ggctctctct
     4921 ccagcatcaa cattttcctt gcggatacgt ctcattacga gagatacggc attagaatct
     4981 gatgggcgtg gaagtccaga tcggcgatgg agcatattga gctgtccgag atgttgttgg
     5041 atcgtcttca ccgccaatcc acgagcttgg aggtagagca agtagtcacg aacgtcttct
     5101 ggctcggctg ggaaccactt gcggttgtta agcttgcacc aggcagccca agaacggcac
     5161 actgagagaa gcatcttcca ggtgtgctcg gagaaggctt gtctgtcacg gaacatatcc
     5221 atgaggttct tgcgaacttc atcacttgtc gcgtcaactg gaagtgctgg aagattttga
     5281 tgaacagtga gaagatttga catattttcg aagtttttta gatgcactag aacaaagcgt
     5341 gttggcttcc tctgagcccg ctttccttat atacccgcat tctgcagcct tacagaatgt
     5401 tctagaaggt cctagatgca ttcgtttgaa aatactcccg gtgggtgcaa agagacgcag
     5461 acggaaaatg tatctgggtc tctttattgt gtacactact tttccatgta ccgaatgtga
     5521 gtcgccctcc ttttgcaaca agcagctcga atgttctaga aaaaggtgga aaatagtata
     5581 aataccgttg aaaataaata ccgaacaaca tttgctctaa ttgtgaaatt agaaatcttc
     5641 aaactataat catgtcactt taccactatt tccgtccagc tcaacgttcc gtttttggtg
     5701 atcatttttg ctttcgtcgt aaatctacac acgcgtctct tccgtgcgag agtccaagcc
     5761 agcagccaaa ttcgttgact gagtattcaa cgtttatacg ttgtcggcaa cgagaaatag
     5821 gaaaatgcat cgggaaatgt tcttttttcg attttttcca aggttttgac aaattttacc
     5881 acgaattttg ctatgttttc aattaaaaaa tatgttattc aactgtttct atgaggaaaa
     5941 taaggctttg catgtaattt tcttattcag cataattttt aattaatttg aattttctgt
     6001 cctaacgttt attttgtttt cttggttatg actgatctga aattaatttt tgaattttaa
     6061 ggtaatatgt caggcggtgc cgcaagtttg tacaaaaaag caggctccat gaaaaagcct
     6121 gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa agttcgacag cgtctccgac
     6181 ctgatgcagc tctcggaggg cgaagaatct cgtgctttca gcttcgatgt aggagggcgt
     6241 ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct acaaagatcg ttatgtttat
     6301 cggcactttg catcggccgc gctcccgatt ccggaagtgc ttgacattgg ggaattcagc
     6361 gagagcctga cctattgcat ctcccgccgt gcacagggtg tcacgttgca agacctgcct
     6421 gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg
     6481 gccgatctta gccagacgag cgggttcggc ccattcggac cgcaaggaat cggtcaatac
     6541 actacatggc gtgatttcat atgcgcgatt gctgatcccc atgtgtatca ctggcaaact
     6601 gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg
     6661 gccgaggact gccccgaagt ccggcacctc gtgcacgcgg atttcggctc caacaatgtc
     6721 ctgacggaca atggccgcat aacagcggtc attgactgga gcgaggcgat gttcggggat
     6781 tcccaatacg aggtcgccaa catcttcttc tggaggccgt ggttggcttg tatggagcag
     6841 cagacgcgct acttcgagcg gaggcatccg gagcttgcag gatcgccgcg gctccgggcg
     6901 tatatgctcc gcattggtct tgaccaactc tatcagagct tggttgacgg caatttcgat
     6961 gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc
     7021 gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga ccgatggctg tgtagaagta
     7081 ctcgccgata gtggaaaccg acgccccagc actcgtccga gggcaaagga atagacccag
     7141 ctttcttgta caaagtgggt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     7201 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     7261 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     7321 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     7381 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     7441 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     7501 cgcaattttt aataacttcg tataatgtat actatacgaa gttattttca gggagccggc
     7561 tcttatccat acgatgtccc agattacgct tacccatatg acgttccaga ctatgccgga
     7621 ggactagttc gacctgcaga ctggctgtgt ataagggagc ctgacattta gattccccag
     7681 aacataaggt tgatagcgtt cttgatatcg ttctcacggt gagaaaggtc ggcaacctcc
     7741 tctccgatga cggaaactgg aacggaagcc atgtcggtgg tcatcatacg ccaagactcg
     7801 tctccgatgt gaacaactgg gtagagctca cgtggaacct tgtcggaaag aagacgggcg
     7861 gaagcaagtg ggataaccat acggcgtcct ggggtatcga tgatgtcgga ttggacgtcg
     7921 acgaaaagac ggtagcgact ctctctttta taagtgtaaa ccttgaattg catttcacca
     7981 gcccctgttc tcgtcggcaa aagagccgtt catttcaata aaccgggcga cctcagccat
     8041 cccttcctga ttttccgctt tccagcgttc ggcacgcaga cgacgggctt cattctgcat
     8101 ggttgtgctt accgaaccgg agatattgac atcatatatg ccttgagcaa ctgatagctg
     8161 tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat acctcttttt gacatacttc
     8221 gggttcctag gaaatcgata ccggtaacat ggtcatagct gtttcctgtg tgaaattgtt
     8281 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     8341 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     8401 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     8461 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     8521 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     8581 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     8641 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     8701 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     8761 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8821 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8881 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8941 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     9001 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     9061 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     9121 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     9181 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     9241 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     9301 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     9361 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     9421 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     9481 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     9541 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     9601 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     9661 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     9721 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9781 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9841 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9901 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9961 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    10021 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    10081 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    10141 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    10201 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    10261 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    10321 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10381 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
    10441 ataaaaatag gcgtatcacg aggccctttc gtc
//
