LOCUS       sequence-135066-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSTN TALEN with 2A-GFP tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Xu L, Zhao P, Mariano A, Han R
  TITLE     Targeted Myostatin Gene Editing in Multiple Mammalian Species 
            Directed by a Single Pair of TALE Nucleases.
  JOURNAL   Mol Ther Nucleic Acids. 2013 Jul 30;2:e112. doi: 
            10.1038/mtna.2013.39.
  PUBMED    23900226
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2013-07-30"; volume: "2:e112. doi"; pages: "
            10.1038/mtna.2013.39"
COMMENT     Sequence Label: pEGFP-2A-TAL6-MSTN
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..585)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNPTQDRILEMKV
                     MEFFMKVYGYRGEHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVKENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNRKTNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEIN"
ORIGIN
        1 gtttatctcg ccgttattaa atttccttct cacttcctct aaggttaatg tgccggcttt
       61 aatcatttct ccaccaatta aaagctcttc tacactaaga acagctccat tacaattagt
      121 cttacgattt aatcgtgtaa gctgagcttt gtagtttcct ttaaagtgac cactcacaaa
      181 taaaaactta aattccgtta cagaagatgg atagactttc caccattcat tagggttgat
      241 atgtttgttt cgtgtttgat tttccttgac atatcgttgc atttcatctg cttggccaat
      301 tggcagatta taacctccgc tataagcttt agtatccacg atcacaccgt aatcaatagg
      361 agatccgaca gtataaattg ctccgtccgg tttccttgat ccacccaaat gttcacctct
      421 atatccataa actttcataa aaaattccat taccttcatt tcaagaatcc tatcctgagt
      481 gggatttctg gcaatttcaa ttaattcaat atattcatga ggcacatatt tcaatttatg
      541 acgaagttca gatttcttct cctccagttc acttttgacc agctgggatc cggcaacgcg
      601 atgggacgtg cgttcgccaa tacggcgatt gactcttctg atcaattccg gcgcgtgcgg
      661 caatcccttt ttcactgcat ccatggcagg acgtccgccg aggcaggcca aggcgacgag
      721 gtggtcgttg gtcaacgcgg ccaacgccgg atcaggccgg ctcagctgg
//
