LOCUS       sequence-135067-         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MSTN TALEN with 2A-GFP tagged.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Xu L, Zhao P, Mariano A, Han R
  TITLE     Targeted Myostatin Gene Editing in Multiple Mammalian Species 
            Directed by a Single Pair of TALE Nucleases.
  JOURNAL   Mol Ther Nucleic Acids. 2013 Jul 30;2:e112. doi: 
            10.1038/mtna.2013.39.
  PUBMED    23900226
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2013-07-30"; volume: "2:e112. doi"; pages: "
            10.1038/mtna.2013.39"
COMMENT     Sequence Label: pEGFP-2A-TAL6-MSTN
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..60
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     CDS             70..135
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             142..162
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 caatgtacta actacgcttt gttgaaactc gctggcgatg ttgaaagtaa ccccggtcct
       61 gaattcatgg actacaaaga ccatgacggt gattataaag atcatgacat cgattacaag
      121 gatgacgatg acaagatggc ccccaagaag aagaggaagg tgggcattca cggggtacct
      181 atggtggatc tacgcacgct cggctacagt cagcagcagc aagagaagat caaaccgaag
      241 gtgcgttcga cagtggcgca gcaccacgag gcactggtgg gccatgggtt tacacacgcg
      301 cacatcgttg cgctcagcca acacccggca gcgttaggga ccgtcgctgt cacgtatcag
      361 cacataatca cggcgttgcc agaggcgaca cacgaagaca tcgttggcgt cggcaaacag
      421 tggtccggcg cacgcgccct ggaggccttg ctcacggatg cgggggagtt gagaggtccg
      481 ccgttacagt tggacacagg ccaacttgtg aagattgcaa aacgtggcgg cgtgaccgca
      541 atggaggcag tgcatgcatc gcgcaatgca ctgacgggtg ctcccctgaa cctgaccccg
      601 gaccaagtgg tggctatcgc cagcaacaat ggcggcaagc aagcgctcga aacggtgcag
      661 cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc
      721 gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
      781 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa caat
//
