LOCUS       sequence-135068-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human dysferlin no transmembrane domain tagged with his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Xu L, Pallikkuth S, Hou Z, Mignery GA, Robia SL, Han R
  TITLE     Dysferlin forms a dimer mediated by the C2 domains and the 
            transmembrane domain in vitro and in living cells.
  JOURNAL   PLoS One. 2011;6(11):e27884. doi: 10.1371/journal.pone.0027884. Epub
            2011 Nov 14.
  PUBMED    22110769
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11):e27884. doi"; pages: " 
            10.1371/journal.pone.0027884. Epub 2011 Nov 14"
COMMENT     Sequence Label: pHis6-hDF-deltaTM-pCMV-9
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             41..58
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             65..82
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatgagc catcaccatc accatcacgc
       61 gtcgctggtt ccgcgtggat ccccaggaat tctgcagtcg acggtaccga tgctgagggt
      121 cttcatcctc tatgccgaga acgtccacac acccgacacc gacatcagcg atgcctactg
      181 ctccgcggtg tttgcagggg tgaagaagag aaccaaagtc atcaagaaca gcgtgaaccc
      241 tgtatggaat gagggatttg aatgggacct caagggcatc cccctggacc agggctctga
      301 gcttcatgtg gtggtcaaag accatgagac gatggggagg aacaggttcc tgggggaagc
      361 caaggtccca ctccgagagg tcctcgccac ccctagtctg tccgccagct tcaatgcccc
      421 cctgctggac accaagaagc agcccacagg ggcctcgctg gtcctgcagg tgtcctacac
      481 accgctgcct ggagctgtgc ctctgttccc gccccctact cctctggagc cctccccgac
      541 tctgcctgac ctggatgtag tggcagacac aggaggagag gaagacacag aggaccaggg
      601 actcactgga gatgaggcgg agccattcct ggatcaaagc ggaggcccgg gggctcccac
      661 caccccaagg aaactacctt cacgtcctcc gccccactac cccgggatca
//
