LOCUS       sequence-135071-       11881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11881)
  AUTHORS   Hibshman JD, Goldstein B
  TITLE     LEA motifs promote desiccation tolerance in vivo.
  JOURNAL   BMC Biol. 2021 Dec 14;19(1):263. doi: 10.1186/s12915-021-01176-0.
  PUBMED    34903234
REFERENCE   2  (bases 1 to 11881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2021-12-14"; volume: "19(1):263. doi"; pages: " 
            10.1186/s12915-021-01176-0"
COMMENT     Sequence Label: pDD317 (Pmyo-2::GFP + SEC)
FEATURES             Location/Qualifiers
     source          1..11881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(2048..2076)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2824..2844
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3289..3309
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    3327..3360
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             3370..3399
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(5216..5539)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    7457..7481
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7484..8509
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7484..8509
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(8510..8534)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    8920..8953
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     CDS             8972..8998
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8999..9025
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9660..9676)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9660..9676)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9673..9695)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9684..9700
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9708..9714,9715..9732,9733..9738))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9753..9774
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9891..9908)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10062..10650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10142..10161)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10821..11612,11613..11681))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11444..11463
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11682..11786)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     11854..11872
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcggga gcctcaggag catcgatttt atatctgagt agtatccttt
     1081 gctttaaatg tccataaaac taattttata atcaataaaa caacgtttgt aaatcaactg
     1141 agtttacaag tagagacatt gagggatact ttcactatgc taaagtgata atcgaccaaa
     1201 taataacttc actttggtat ttattcctgt cttataaatg ttatgtatga attaaattca
     1261 tatgcatatg gctcactctg acaattttta ataatcttcc agatcaatat tgactaccga
     1321 tgcgggtggt cttttgcttt gaattctgct gaactttaca ccccgaacag caatgtgtgc
     1381 ttcagcctaa aaaaaagtaa gtgtgttaat cagtgccccc gattcttcat tttttgcccc
     1441 tctctcccgt ttcgtcggca aaagaagaga aaataaagat aagtctcaag ataggttggt
     1501 aatcgctaaa gtggttgtgt ggataagagt agcaaaatgg caggaagagc actttgcgcg
     1561 cacacactgt actcattgtt ctggataaaa ttctctcgtt gtttgccgtc ggatgtctgc
     1621 ctctctgcat tgagccggct tcttcactat ctttagttaa cctaaaatgc cgtttctttt
     1681 ctcgtatccc cactatcccg ttgaggttct ctgctctctt cgctccctac cgccagcgag
     1741 caactatccg tgggggcgcc ttgctcggaa gatggggggg aagaaagaag atttttgcta
     1801 tttgcacttg agaaagagac ttttcctgcg tcgatggtta gagaacagtg tgcagacact
     1861 tttcagctac ctagaattac aattggatat ccccgcctcc caatccaccc acccagggaa
     1921 aaagaagggc tcgccgaaaa tcaaagttat ctccaggctc gcgcatccca ccgagcggtt
     1981 gacttctctc caccactttt catttttacc ctcgaccatg agtaaaggag aagaactttt
     2041 cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc
     2101 tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta aatttatttg
     2161 cactactgga aaactacctg ttccatgggt aagtttaaac atatatatac taactaaccc
     2221 tgattattta aattttcagc caacacttgt cactactttc tgttatggtg ttcaatgctt
     2281 ctcgagatac ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg
     2341 ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtaagtt
     2401 taaacagttc ggtactaact aaccatacat atttaaattt tcaggtgctg aagtcaagtt
     2461 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     2521 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     2581 agacaaacaa aagaatggaa tcaaagttgt aagtttaaac atgattttac taactaacta
     2641 atctgattta aattttcaga acttcaaaat tagacacaac attgaagatg gaagcgttca
     2701 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
     2761 caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa agagagacca
     2821 catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg atgaactata
     2881 caaatagatc aactgctcta attccatctc gtttctctct tttctttcaa tctctttacc
     2941 cgtttctctt cccctacgtc ccccaaacct accactgcac agtaacacat acacattcaa
     3001 cgtgtcacaa taaaagccca tcaacacctt ctctgttgtt ttttttcttc aaatgcattt
     3061 ttttcacttt gtttaataat ttttacgcat cggatttgta ggatagtgga aatttgtcct
     3121 tcaatgcttc aattgattcg acacttctca atatttttga tcaataaacc attgattttc
     3181 tatttagaag aagatcactg taaataacac caaaaacatc acagcaaaaa aactcccact
     3241 caattatatt actgttttta ttgaaactag aataaatttg aaaccggtga gaatctgtac
     3301 tttcaatccg gaaaggtaag tttaaaataa cttcgtataa tgtatactat acgaagttat
     3361 tttcagggag agcaaaagct tatctccgag gaggacctct aaggatgatc gacgccaacg
     3421 tcgttgaatt ttcaaatttt aaatactgaa tatttgtttt ttttcctatt atttatttat
     3481 tctctttgtg ttttttttct tgctttctaa aaaattaatt caatccaaat ctaaacattt
     3541 ttttttctct ttccgtctcc caattcgtat tccgctcctc tcatctgaac acaatgtgca
     3601 agtttattta tcttctcgct ttcatttcat taggacgtgg ggggaattgg tggaaggggg
     3661 aaacacacaa aaggatgatg gaaatgaaat aaggacacac aatatgcaac aacattcaat
     3721 tcagaaatat ggaggaaggt ttaaaagaaa acataaaaat atatagagga ggaaggaaac
     3781 tagctgccct cgattgtcta tcttccattc tcaccaagtt tcaacaactt tattcagtca
     3841 ttcttctctg gttcatttcc caaccaatct tcccgaataa agcccatctg tcctttcggc
     3901 cacattcctc acctacatcc gtactcctta tctcccgttc ttatccagtc ttttatcgtc
     3961 tctgcgtctc gcacactgtc tcgtcggtaa tgtaaaaaaa aaacaaaaaa aacttgaaaa
     4021 cttgcggaaa gatttcctaa taaaaggaga cgaatccggt aggaaatcga caagtctctg
     4081 cttctcgaag actaggtgag ttacatttct ttggccgaat tattttgaaa tcctaaattt
     4141 gataatttca gagatgtctg taaaacttgc gtgttatgtg acggcttcgg tcactgtcgc
     4201 cactcttatg gtatgcttca tgaccatgtc aaccatctac tcggaggttg atggattcag
     4261 agagaagctt gataccgaga tgaatgtgtt cagagtgagt taaattatat tttgaatttt
     4321 aaataatttt taattttcta gcaatctacc aatggattgt ggaaggacat agttgtcatc
     4381 ggaagatcta gcaagcgtgt ccgttgtcaa tatgaagaga ccaacgctac cccaactcca
     4441 catgctgatg gatccccatc tgctccacca ggtcaaccac cagcagttcc accagtcttc
     4501 aaccagccaa agactccaaa tggagccaat ggaaatggac caacctgcaa ctgcaatgct
     4561 gataacaagt gcccagctgg accatccgga ccaaagggag ttccaggagt tccaggactc
     4621 gacggagttc caggacttga cggtgttcca ggagttggag ctgatgatat cgctccacaa
     4681 cgcgagtctg tcggatgctt cacttgccca caaggaccag ttggaccacc aggagctctt
     4741 ggaagaccag gaccacgtgg acttccagga ccaagaggac aaaatggaaa cccaggaaga
     4801 gatggacaac caggacatcc aggagagcaa ggatcatccg gtcaaatcgg aaagatcgga
     4861 gagccaggac caccaggaga gaagggacgc gacgccgagc atccaatcgg aagaccagga
     4921 ccaaagggac caagaggaga tcaaggacca acaggaccag ctggacagaa cggtcttcac
     4981 ggaccaccag gagagccagg aaccgttgga ccagaaggac catctggaaa gcaaggacgt
     5041 caaggaccag acggaaccca gggagagact ggaccagacg gaagaccagg aaaggatgcc
     5101 gagtactgcc agtgcccaga caagtctcca ccatcagagg ctgtcaacgc caaccgtgga
     5161 tacagaaata tctaaattgt tggtgttttc taataaaata tttgagattc agtgagactt
     5221 ttttcttggc ggcacaataa agtttcctct ttttgttggg tcactcaacg aacacaatac
     5281 aaatatttga gcgctcgcat ttttctcaac ctttcgggtt taaagcactt caaaatggaa
     5341 ttgaagagag attgtgaaag tacatcagac agaatcacat tttgatagca ttcacttcac
     5401 tcagatgcaa acttttcagt gactataaaa agtcataaaa taataacatt aaaaaagcaa
     5461 ataaattagc gagagaattt tttgacaaaa agaaagaaga gtgatagaga agaagggaat
     5521 gcttgaaagg attttgcatt tatcttaatc accgtcttcg agaagacgaa ccatggcgcc
     5581 agtttcagaa tccaagtttc taatatagtt catcacaatg ttgacgttgg tccaacctcc
     5641 ggcttgcata atctctggga tggaaactcc agcgcgagcc atgtcacggg cagctccgac
     5701 acgggcggaa tgtccagacc aagccaagta acgttgtccg gaatcatcct tggctccgta
     5761 gatgagacgg tgggtagctt cgaagattcc tgaaaattta ttttcagctt attcttagct
     5821 aatatgttgt ttaaacttac cttcaagagc tcgagtcgag agttgtgatg ttgcactcgg
     5881 ggcagcaact ccgttcttgc gaacacgaca gaacaggtag ttgtttggat catcggcgac
     5941 tccggaaacc gaaatccagc gctcaacgag cttggtaact ccaagggaga gcgctttttc
     6001 gactccggca gtcgagacca gcgtttttgt acgtccgatg tggatgagca ttcttcctcc
     6061 gtctgtgcga ctaatatcct tgacgcgaat gcgggcaatt tcggcgatac ggagaagtgt
     6121 gttgtaagca attccgagga aggcgaggtt tcggatgtcc tggcaacgat cggaattttc
     6181 cataagagaa cggacctgat cgaagtcggt tctgaaaatt tcgtcaagtc ttaggattga
     6241 atacattaat atttaaactt acctttcgaa ggcgagggct tgcttggctc tctctccagc
     6301 atcaacattt tccttgcgga tacgtctcat tacgagagat acggcattag aatctgatgg
     6361 gcgtggaagt ccagatcggc gatggagcat attgagctgt ccgagatgtt gttggatcgt
     6421 cttcaccgcc aatccacgag cttggaggta gagcaagtag tcacgaacgt cttctggctc
     6481 ggctgggaac cacttgcggt tgttaagctt gcaccaggca gcccaagaac ggcacactga
     6541 gagaagcatc ttccaggtgt gctcggagaa ggcttgtctg tcacggaaca tatccatgag
     6601 gttcttgcga acttcatcac ttgtcgcgtc aactggaagt gctggaagat tttgatgaac
     6661 agtgagaaga tttgacatat tttcgaagtt ttttagatgc actagaacaa agcgtgttgg
     6721 cttcctctga gcccgctttc cttatatacc cgcattctgc agccttacag aatgttctag
     6781 aaggtcctag atgcattcgt ttgaaaatac tcccggtggg tgcaaagaga cgcagacgga
     6841 aaatgtatct gggtctcttt attgtgtaca ctacttttcc atgtaccgaa tgtgagtcgc
     6901 cctccttttg caacaagcag ctcgaatgtt ctagaaaaag gtggaaaata gtataaatac
     6961 cgttgaaaat aaataccgaa caacatttgc tctaattgtg aaattagaaa tcttcaaact
     7021 ataatcatgt cactttacca ctatttccgt ccagctcaac gttccgtttt tggtgatcat
     7081 ttttgctttc gtcgtaaatc tacacacgcg tctcttccgt gcgagagtcc aagccagcag
     7141 ccaaattcgt tgactgagta ttcaacgttt atacgttgtc ggcaacgaga aataggaaaa
     7201 tgcatcggga aatgttcttt tttcgatttt ttccaaggtt ttgacaaatt ttaccacgaa
     7261 ttttgctatg ttttcaatta aaaaatatgt tattcaactg tttctatgag gaaaataagg
     7321 ctttgcatgt aattttctta ttcagcataa tttttaatta atttgaattt tctgtcctaa
     7381 cgtttatttt gttttcttgg ttatgactga tctgaaatta atttttgaat tttaaggtaa
     7441 tatgtcaggc ggtgccgcaa gtttgtacaa aaaagcaggc tccatgaaaa agcctgaact
     7501 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     7561 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     7621 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     7681 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     7741 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     7801 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     7861 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     7921 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     7981 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     8041 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     8101 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     8161 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     8221 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     8281 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     8341 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     8401 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     8461 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaataga cccagctttc
     8521 ttgtacaaag tgggtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc
     8581 ccacccccta tttttgttat tatcaaaaaa cttctcttaa tttctttgtt ttttagcttc
     8641 ttttaagtca cctctaacaa tgaaattgtg tagattcaaa aatagaatta attcgtaata
     8701 aaaagtcgaa aaaaattgtg ctccctcccc ccattaataa taattctatc ccaaaatcta
     8761 cacaatgttc tgtgtacact tcttatgttt tttacttctg ataaattttt ttgaaacatc
     8821 atagaaaaaa ccgcacacaa aataccttat catatgttac gtttcagttt atgaccgcct
     8881 tatcatatgt tacgtttcag tttatgaccg caatttttaa taacttcgta taatgtatac
     8941 tatacgaagt tattttcagg gagccggctc ttatccatac gatgtcccag attacgctta
     9001 cccatatgac gttccagact atgccggagg actagttcga cctgcagact ggctgtgtat
     9061 aagggagcct gacatttaga ttccccagaa cataaggttg atagcgttct tgatatcgtt
     9121 ctcacggtga gaaaggtcgg caacctcctc tccgatgacg gaaactggaa cggaagccat
     9181 gtcggtggtc atcatacgcc aagactcgtc tccgatgtga acaactgggt agagctcacg
     9241 tggaaccttg tcggaaagaa gacgggcgga agcaagtggg ataaccatac ggcgtcctgg
     9301 ggtatcgatg atgtcggatt ggacgtcgac gaaaagacgg tagcgactct ctcttttata
     9361 agtgtaaacc ttgaattgca tttcaccagc ccctgttctc gtcggcaaaa gagccgttca
     9421 tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg
     9481 cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cgaaccggag atattgacat
     9541 catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa tacgctgctt
     9601 catagcatac ctctttttga catacttcgg gttcctagga aatcgatacc ggtaacatgg
     9661 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     9721 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     9781 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     9841 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     9901 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     9961 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
    10021 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
    10081 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
    10141 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
    10201 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
    10261 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
    10321 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
    10381 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
    10441 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
    10501 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
    10561 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
    10621 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
    10681 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
    10741 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
    10801 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
    10861 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
    10921 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
    10981 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
    11041 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
    11101 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
    11161 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
    11221 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
    11281 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
    11341 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
    11401 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
    11461 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
    11521 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
    11581 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
    11641 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
    11701 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
    11761 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
    11821 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
    11881 c
//
