LOCUS       sequence-135073-       14888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains left and right homology arms for mouse GDF8 
            flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14888)
  AUTHORS   Xu L, Zhao P, Mariano A, Han R
  TITLE     Targeted Myostatin Gene Editing in Multiple Mammalian Species 
            Directed by a Single Pair of TALE Nucleases.
  JOURNAL   Mol Ther Nucleic Acids. 2013 Jul 30;2:e112. doi: 
            10.1038/mtna.2013.39.
  PUBMED    23900226
REFERENCE   2  (bases 1 to 14888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2013-07-30"; volume: "2:e112. doi"; pages: "
            10.1038/mtna.2013.39"
COMMENT     Sequence Label: mGDF8 donor CFP-hDF
FEATURES             Location/Qualifiers
     source          1..14888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1111..1414
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1415..1618
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1569..1589
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      1657..1666
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1663..2370
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1808..1826)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2076..2095
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             2416..3012
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2416..2435)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2912..2932
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3031..3084
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      3097..3106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3103..3105,3106..3108,3109..3819)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3148..3169)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3409..3428)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3756..3777
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4522..4533
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10258..10379
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10295..10314)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10349..10368
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11260..11279)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11261..11279)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11286..11303)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11286..11302)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11295..11317)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             11440..11742
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11621..11640
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             12091..12885
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             12091..12885
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(12145..12164)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12755..12774
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(13135..13926,13927..13995))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13758..13777
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14119..14707
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14608..14627
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14861..14878
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtcaa ggccactgct ttctgatttt ttagatagct
      301 tgagcatgac ctctgaggtt tttgctaatt aagtttttgt tgttccttgg aatagctctt
      361 ccagagccca agtgtcacag atgtattata ttgtttgcca tttctgaggt tggttaaatc
      421 gctccctgtg tcttgtctcc aggtaattga ggcctggggg aagggttcct tcctccagac
      481 tgactggtac agctgctcag tgagtgtaac tgctcagatt cccaaagaat tctaagtgga
      541 cgttccggca cgctgtctct tagtctctaa tcatcatcac ttgaaaattt catgtgctgt
      601 tcattctttt acaaaatatc ccttagccca gagttctctg gaaggggagt agaatctaca
      661 taaacaggtg aaaatacaga tgtcaaaaaa aaaaattctg aaaagctaga gcaactattt
      721 tctctgatgt ttcttctgaa ctatgaatga agttccacat aatattttat tttaaaagac
      781 cacatacgta cacactaccc caacagctga tgttaaggag tgagtttatt tgtgttttga
      841 tggggcctga tgtcagtcat tttcttctta ttcctttgta gctgactttc taatgcaagc
      901 ggatggcaag cccaaatgtt gcttttttaa atttagctct aaaatacagt acaacaaagt
      961 agtaaaagcc caactgtgga tatatctcag acccgtcaag actcctacaa cagtgtttct
     1021 cgagagatcc gaattcatgc ggccgcagtc tagatattaa tagtaatcaa ttacggggtc
     1081 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     1141 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     1201 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     1261 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     1321 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     1381 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     1441 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     1501 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     1561 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
     1621 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag
     1681 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
     1741 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
     1801 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
     1861 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
     1921 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
     1981 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
     2041 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
     2101 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
     2161 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
     2221 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
     2281 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     2341 tccaccggcg gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg
     2401 aattctgcag tcgagatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac
     2461 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac
     2521 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg
     2581 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc
     2641 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg
     2701 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg
     2761 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag
     2821 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg
     2881 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc
     2941 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc
     3001 aagcccggtg ccggcggcgg gtccggagga gagggcagag gaagtcttct aacatgcggt
     3061 gacgtggagg agaatcctgg cccaatggga ccggtcgcca ccatggtgag caagggcgag
     3121 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     3181 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     3241 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     3301 tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     3361 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     3421 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     3481 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     3541 atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa ggccaacttc
     3601 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     3661 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     3721 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     3781 gccgccggga tcactctcgg catggacgag ctgtacaagc gaattctgca gtcgacggta
     3841 ccgatgctga gggtcttcat cctctatgcc gagaacgtcc acacacccga caccgacatc
     3901 agcgatgcct actgctccgc ggtgtttgca ggggtgaaga agagaaccaa agtcatcaag
     3961 aacagcgtga accctgtatg gaatgaggga tttgaatggg acctcaaggg catccccctg
     4021 gaccagggct ctgagcttca tgtggtggtc aaagaccatg agacgatggg gaggaacagg
     4081 ttcctggggg aagccaaggt cccactccga gaggtcctcg ccacccctag tctgtccgcc
     4141 agcttcaatg cccccctgct ggacaccaag aagcagccca caggggcctc gctggtcctg
     4201 caggtgtcct acacaccgct gcctggagct gtgcctctgt tcccgccccc tactcctctg
     4261 gagccctccc cgactctgcc tgacctggat gtagtggcag acacaggagg agaggaagac
     4321 acagaggacc agggactcac tggagatgag gcggagccat tcctggatca aagcggaggc
     4381 ccgggggctc ccaccacccc aaggaaacta ccttcacgtc ctccgcccca ctaccccggg
     4441 atcaaaagaa agcgaagtgc gcctacatct agaaagctgc tgtcagacaa accgcaggat
     4501 ttccagatca gggtccaggt gatcgagggg cgccagctgc cgggggtgaa catcaagcct
     4561 gtggtcaagg ttaccgctgc agggcagacc aagcggacgc ggatccacaa gggaaacagc
     4621 ccactcttca atgagactct tttcttcaac ttgtttgact ctcctgggga gctgtttgat
     4681 gagcccatct ttatcacggt ggtagactct cgttctctca ggacagatgc tctcctcggg
     4741 gagttccgga tggacgtggg caccatttac agagagcccc ggcacgccta tctcaggaag
     4801 tggctgctgc tctcagaccc tgatgacttc tctgctgggg ccagaggcta cctgaaaaca
     4861 agcctttgtg tgctggggcc tggggacgaa gcgcctctgg agagaaaaga cccctctgaa
     4921 gacaaggagg acattgaaag caacctgctc cggcccacag gcgtagccct gcgaggagcc
     4981 cacttctgcc tgaaggtctt ccgggccgag gacttgccgc agatggacga tgccgtgatg
     5041 gacaacgtga aacagatctt tggcttcgag agtaacaaga agaacttggt ggaccccttt
     5101 gtggaggtca gctttgcggg gaaaatgctg tgcagcaaga tcttggagaa gacggccaac
     5161 cctcagtgga accagaacat cacactgcct gccatgtttc cctccatgtg cgaaaaaatg
     5221 aggattcgta tcatagactg ggaccgcctg actcacaatg acatcgtggc taccacctac
     5281 ctgagtatgt cgaaaatctc tgcccctgga ggagaaatag aagaggagcc tgcaggtgct
     5341 gtcaagcctt cgaaagcctc agacttggat gactacctgg gcttcctccc cacttttggg
     5401 ccctgctaca tcaacctcta tggcagtccc agagagttca caggcttccc agacccctac
     5461 acagagctca acacaggcaa gggggaaggt gtggcttatc gtggccggct tctgctctcc
     5521 ctggagacca agctggtgga gcacagtgaa cagaaggtgg aggaccttcc tgcggatgac
     5581 atcctccggg tggagaagta ccttaggagg cgcaagtact ccctgtttgc ggccttctac
     5641 tcagccacca tgctgcagga tgtggatgat gccatccagt ttgaggtcag catcgggaac
     5701 tacgggaaca agttcgacat gacctgcctg ccgctggcct ccaccactca gtacagccgt
     5761 gcagtctttg acgggtgcca ctactactac ctaccctggg gtaacgtgaa acctgtggtg
     5821 gtgctgtcat cctactggga ggacatcagc catagaatcg agactcagaa ccagctgctt
     5881 gggattgctg accggctgga agctggcctg gagcaggtcc acctggccct gaaggcgcag
     5941 tgctccacgg aggacgtgga ctcgctggtg gctcagctga cggatgagct catcgcaggc
     6001 tgcagccagc ctctgggtga catccatgag acaccctctg ccacccacct ggaccagtac
     6061 ctgtaccagc tgcgcaccca tcacctgagc caaatcactg aggctgccct ggccctgaag
     6121 ctcggccaca gtgagctccc tgcagctctg gagcaggcgg aggactggct cctgcgtctg
     6181 cgtgccctgg cagaggagcc ccagaacagc ctgccggaca tcgtcatctg gatgctgcag
     6241 ggagacaagc gtgtggcata ccagcgggtg cccgcccacc aagtcctctt ctcccggcgg
     6301 ggtgccaact actgtggcaa gaattgtggg aagctacaga caatctttct gaaatatccg
     6361 atggagaagg tgcctggcgc ccggatgcca gtgcagatac gggtcaagct gtggtttggg
     6421 ctctctgtgg atgagaagga gttcaaccag tttgctgagg ggaagctgtc tgtctttgct
     6481 gaaacctatg agaacgagac taagttggcc cttgttggga actggggcac aacgggcctc
     6541 acctacccca agttttctga cgtcacgggc aagatcaagc tacccaagga cagcttccgc
     6601 ccctcggccg gctggacctg ggctggagat tggttcgtgt gtccggagaa gactctgctc
     6661 catgacatgg acgccggtca cctgagcttc gtggaagagg tgtttgagaa ccagacccgg
     6721 cttcccggag gccagtggat ctacatgagt gacaactaca ccgatgtgaa cggggagaag
     6781 gtgcttccca aggatgacat tgagtgccca ctgggctgga agtgggaaga tgaggaatgg
     6841 tccacagacc tcaaccgggc tgtcgatgag caaggctggg agtatagcat caccatcccc
     6901 ccggagcgga agccgaagca ctgggtccct gctgagaaga tgtactacac acaccgacgg
     6961 cggcgctggg tgcgcctgcg caggagggat ctcagccaaa tggaagcact gaaaaggcac
     7021 aggcaggcgg aggcggaggg cgagggctgg gagtacgcct ctctttttgg ctggaagttc
     7081 cacctcgagt accgcaagac agatgccttc cgccgccgcc gctggcgccg tcgcatggag
     7141 ccactggaga agacggggcc tgcagctgtg tttgcccttg agggggccct gggcggcgtg
     7201 atggatgaca agagtgaaga ttccatgtcc gtctccacct tgagcttcgg tgtgaacaga
     7261 cccacgattt cctgcatatt cgactatggg aaccgctacc atctacgctg ctacatgtac
     7321 caggcccggg acctggctgc gatggacaag gactcttttt ctgatcccta tgccatcgtc
     7381 tccttcctgc accagagcca gaagacggtg gtggtgaaga acacccttaa ccccacctgg
     7441 gaccagacgc tcatcttcta cgagatcgag atctttggcg agccggccac agttgctgag
     7501 caaccgccca gcattgtggt ggagctgtac gaccatgaca cttatggtgc agacgagttt
     7561 atgggtcgct gcatctgtca accgagtctg gaacggatgc cacggctggc ctggttccca
     7621 ctgacgaggg gcagccagcc gtcgggggag ctgctggcct cttttgagct catccagaga
     7681 gagaagccgg ccatccacca tattcctggt tttgaggtgc aggagacatc aaggatcctg
     7741 gatgagtctg aggacacaga cctgccctac ccaccacccc agagggaggc caacatctac
     7801 atggttcctc agaacatcaa gccagcgctc cagcgtaccg ccatcgagat cctggcatgg
     7861 ggcctgcgga acatgaagag ttaccagctg gccaacatct cctcccccag cctcgtggta
     7921 gagtgtgggg gccagacggt gcagtcctgt gtcatcagga acctccggaa gaaccccaac
     7981 tttgacatct gcaccctctt catggaagtg atgctgccca gggaggagct ctactgcccc
     8041 cccatcaccg tcaaggtcat cgataaccgc cagtttggcc gccggcctgt ggtgggccag
     8101 tgtaccatcc gctccctgga gagcttcctg tgtgacccct actcggcgga gagtccatcc
     8161 ccacagggtg gcccagacga tgtgagccta ctcagtcctg gggaagacgt gctcatcgac
     8221 attgatgaca aggagcccct catccccatc caggaggaag agttcatcga ttggtggagc
     8281 aaattctttg cctccatagg ggagagggaa aagtgcggct cctacctgga gaaggatttt
     8341 gacaccctga aggtctatga cacacagctg gagaatgtgg aggcctttga gggcctgtct
     8401 gacttttgta acaccttcaa gctgtaccgg ggcaagacgc aggaggagac agaagatcca
     8461 tctgtgattg gtgaatttaa gggcctcttc aaaatttatc ccctcccaga agacccagcc
     8521 atccccatgc ccccaagaca gttccaccag ctggccgccc agggacccca ggagtgcttg
     8581 gtccgtatct acattgtccg agcatttggc ctgcagccca aggaccccaa tggaaagtgt
     8641 gatccttaca tcaagatctc catagggaag aaatcagtga gtgaccagga taactacatc
     8701 ccctgcacgc tggagcccgt atttggaaag atgttcgagc tgacctgcac tctgcctctg
     8761 gagaaggacc taaagatcac tctctatgac tatgacctcc tctccaagga cgaaaagatc
     8821 ggtgagacgg tcgtcgacct ggagaacagg ctgctgtcca agtttggggc tcgctgtgga
     8881 ctcccacaga cctactgtgt ctctggaccg aaccagtggc gggaccagct ccgcccctcc
     8941 cagctcctcc acctcttctg ccagcagcat agagtcaagg cacctgtgta ccggacagac
     9001 cgtgtaatgt ttcaggataa agaatattcc attgaagaga tagaggctgg caggatccca
     9061 aacccacacc tgggcccagt ggaggagcgt ctggctctgc atgtgcttca gcagcagggc
     9121 ctggtcccgg agcacgtgga gtcacggccc ctctacagcc ccctgcagcc agacatcgag
     9181 caggggaagc tgcagatgtg ggtcgaccta tttccgaagg ccctggggcg gcctggacct
     9241 cccttcaaca tcaccccacg gagagccaga aggtttttcc tgcgttgtat tatctggaat
     9301 accagagatg tgatcctgga tgacctgagc ctcacggggg agaagatgag cgacatttat
     9361 gtgaaaggtt ggatgattgg ctttgaagaa cacaagcaaa agacagacgt gcattatcgt
     9421 tccctgggag gtgaaggcaa cttcaactgg aggttcattt tccccttcga ctacctgcca
     9481 gctgagcaag tctgtaccat tgccaagaag gatgccttct ggaggctgga caagactgag
     9541 agcaaaatcc cagcacgagt ggtgttccag atctgggaca atgacaagtt ctcctttgat
     9601 gattttctgg gctccctgca gctcgatctc aaccgcatgc ccaagccagc caagacagcc
     9661 aagaagtgct ccttggacca gctggatgat gctttccacc ccgaatggtt tgtgtccctt
     9721 tttgagcaga aaacagtgaa gggctggtgg ccctgtgtag cagaagaggg tgagaagaaa
     9781 atactggcgg gcaagctgga aatgaccttg gagattgtag cagagagtga gcatgaggag
     9841 cggcctgctg gccagggccg ggatgagccc aacatgaacc ctaagcttga ggacccaagg
     9901 cgccccgaca cctccttcct gtggtttacc tccccataca agaccatgaa gttcatcctg
     9961 tggcggcgtt tccggtgggc catcatcctc ttcatcatcc tcttcatcct gctgctgttc
    10021 ctggccatct tcatctacgc cttcccgaac tatgctgcca tgaagctggt gaagcccttc
    10081 agctgaggac tctcctgccc tgtagaaggg gccggtaccg cgggcccggg atccaccgga
    10141 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
    10201 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
    10261 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
    10321 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttag
    10381 atatccgatc tctgaaactt gacatgagcc caggcactgg tatttggcag agtattgatg
    10441 tgaagacagt gttgcaaaat tggctcaaac agcctgaatc caacttaggc attgaaatca
    10501 aagctttgga tgagaatggc catgatcttg ctgtaacctt cccaggacca ggagaagatg
    10561 ggctggtaag tgataactga aaataacatt ctagcaaccg tgttacattt ttattcctga
    10621 catgaatgat aatagtgaaa aagaaatact atttcctatg ctcataagcc agacaaagta
    10681 tcttactcca aaggtaggtg tgtacccaaa acagtacaca cctacctttg gtgtgtcaat
    10741 ctcatgtccc atgagggaca tgaacccctt ggtgttctta cttagcatga agaccaaaaa
    10801 ccaattacat catgttccta attccacaag gaggtcctta ctaggaatta gatgtaatgt
    10861 ccttttcatt gtaagtcaca taattatgta taaactatga agtctctatg taactgtaga
    10921 ttctggtttc tacccaacaa attaaaacct ttccttcccg ttttatgttt ctttcctatg
    10981 ttttatattg aaaaatgttg gttccacata aagtaaaaaa atattaaaat tctaattaaa
    11041 atacccttat atgtatagat atacttgatt aaatatatta tactcaacac ttctagtaaa
    11101 tatgttcagt acttatagaa tgttgaagcc atgatatgta tatagattac tttgtgaaat
    11161 attgtcaata aaactgtaat ttctaaccaa gctaaccttt gcctcctagc ttatctttgg
    11221 actcctttag catacttgcg cggccgctaa atcaattcgc cctatagtga gtcgtattac
    11281 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
    11341 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
    11401 gatcgccctt cccaacagtt gcgcagccta tacgtacggc agtttaaggt ttacacctat
    11461 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccg
    11521 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt
    11581 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
    11641 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
    11701 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg catgagatta
    11761 tcaaaaagga tcttcaccta gatccttttc acgtagaaag ccagtccgca gaaacggtgc
    11821 tgaccccgga tgaatgtcag ctactgggct atctggacaa gggaaaacgc aagcgcaaag
    11881 agaaagcagg tagcttgcag tgggcttaca tggcgatagc tagactgggc ggttttatgg
    11941 acagcaagcg aaccggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc
    12001 aaagtaaact ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagctct
    12061 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
    12121 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
    12181 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
    12241 accgacctgt ccggtgccct gaatgaactg caagacgagg cagcgcggct atcgtggctg
    12301 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
    12361 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
    12421 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
    12481 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
    12541 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
    12601 ttcgccaggc tcaaggcgag catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
    12661 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
    12721 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
    12781 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
    12841 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgaattat taacgcttac
    12901 aatttcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg
    12961 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    13021 aaatatgtat ccgctcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
    13081 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    13141 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    13201 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    13261 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    13321 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    13381 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    13441 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    13501 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    13561 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    13621 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    13681 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    13741 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    13801 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    13861 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    13921 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    13981 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    14041 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    14101 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    14161 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    14221 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    14281 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    14341 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
    14401 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
    14461 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
    14521 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
    14581 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
    14641 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
    14701 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
    14761 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
    14821 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
    14881 agcggaag
//
