LOCUS       sequence-135082-       13133 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  human dysferlin no transmembrane domain tagged with YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13133)
  AUTHORS   Xu L, Pallikkuth S, Hou Z, Mignery GA, Robia SL, Han R
  TITLE     Dysferlin forms a dimer mediated by the C2 domains and the 
            transmembrane domain in vitro and in living cells.
  JOURNAL   PLoS One. 2011;6(11):e27884. doi: 10.1371/journal.pone.0027884. Epub
            2011 Nov 14.
  PUBMED    22110769
REFERENCE   2  (bases 1 to 13133)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(11):e27884. doi"; pages: " 
            10.1371/journal.pone.0027884. Epub 2011 Nov 14"
COMMENT     Sequence Label: EYFP-hDF-deltaTM-pCMV-9
FEATURES             Location/Qualifiers
     source          1..13133
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     140..157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     852..872
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      940..949
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(946..948,949..951,952..1662)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(991..1012)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1252..1271)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1599..1620
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             2389..2400
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7874..8496
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(8073..8092)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(8520..8540)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8538..8836
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             8890..9684
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8944..8963)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9554..9573
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10336..10470
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10373..10392)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10427..10446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10475..10494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10476..10494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10508..10524)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10508..10524)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10521..10543)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10532..10548
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10556..10562,10563..10580,10581..10586))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10601..10622
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10739..10756)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10910..11498)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10990..11009)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11669..12460,12461..12529))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12292..12311
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12530..12634)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12748..12767)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12975..12996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
       61 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      121 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
      181 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
      241 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      301 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
      361 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      421 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      481 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      541 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
      601 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
      661 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
      721 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      781 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
      841 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      901 tggtttagtg aaccgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
      961 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     1021 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     1081 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccttc
     1141 ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc
     1201 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     1261 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     1321 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     1381 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     1441 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     1501 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag
     1561 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     1621 accgccgccg ggatcactct cggcatggac gagctgtaca agtactcaga tctcgagctc
     1681 aagcttcgaa ttctgcagtc gacggtaccg atgctgaggg tcttcatcct ctatgccgag
     1741 aacgtccaca cacccgacac cgacatcagc gatgcctact gctccgcggt gtttgcaggg
     1801 gtgaagaaga gaaccaaagt catcaagaac agcgtgaacc ctgtatggaa tgagggattt
     1861 gaatgggacc tcaagggcat ccccctggac cagggctctg agcttcatgt ggtggtcaaa
     1921 gaccatgaga cgatggggag gaacaggttc ctgggggaag ccaaggtccc actccgagag
     1981 gtcctcgcca cccctagtct gtccgccagc ttcaatgccc ccctgctgga caccaagaag
     2041 cagcccacag gggcctcgct ggtcctgcag gtgtcctaca caccgctgcc tggagctgtg
     2101 cctctgttcc cgccccctac tcctctggag ccctccccga ctctgcctga cctggatgta
     2161 gtggcagaca caggaggaga ggaagacaca gaggaccagg gactcactgg agatgaggcg
     2221 gagccattcc tggatcaaag cggaggcccg ggggctccca ccaccccaag gaaactacct
     2281 tcacgtcctc cgccccacta ccccgggatc aaaagaaagc gaagtgcgcc tacatctaga
     2341 aagctgctgt cagacaaacc gcaggatttc cagatcaggg tccaggtgat cgaggggcgc
     2401 cagctgccgg gggtgaacat caagcctgtg gtcaaggtta ccgctgcagg gcagaccaag
     2461 cggacgcgga tccacaaggg aaacagccca ctcttcaatg agactctttt cttcaacttg
     2521 tttgactctc ctggggagct gtttgatgag cccatcttta tcacggtggt agactctcgt
     2581 tctctcagga cagatgctct cctcggggag ttccggatgg acgtgggcac catttacaga
     2641 gagccccggc acgcctatct caggaagtgg ctgctgctct cagaccctga tgacttctct
     2701 gctggggcca gaggctacct gaaaacaagc ctttgtgtgc tggggcctgg ggacgaagcg
     2761 cctctggaga gaaaagaccc ctctgaagac aaggaggaca ttgaaagcaa cctgctccgg
     2821 cccacaggcg tagccctgcg aggagcccac ttctgcctga aggtcttccg ggccgaggac
     2881 ttgccgcaga tggacgatgc cgtgatggac aacgtgaaac agatctttgg cttcgagagt
     2941 aacaagaaga acttggtgga cccctttgtg gaggtcagct ttgcggggaa aatgctgtgc
     3001 agcaagatct tggagaagac ggccaaccct cagtggaacc agaacatcac actgcctgcc
     3061 atgtttccct ccatgtgcga aaaaatgagg attcgtatca tagactggga ccgcctgact
     3121 cacaatgaca tcgtggctac cacctacctg agtatgtcga aaatctctgc ccctggagga
     3181 gaaatagaag aggagcctgc aggtgctgtc aagccttcga aagcctcaga cttggatgac
     3241 tacctgggct tcctccccac ttttgggccc tgctacatca acctctatgg cagtcccaga
     3301 gagttcacag gcttcccaga cccctacaca gagctcaaca caggcaaggg ggaaggtgtg
     3361 gcttatcgtg gccggcttct gctctccctg gagaccaagc tggtggagca cagtgaacag
     3421 aaggtggagg accttcctgc ggatgacatc ctccgggtgg agaagtacct taggaggcgc
     3481 aagtactccc tgtttgcggc cttctactca gccaccatgc tgcaggatgt ggatgatgcc
     3541 atccagtttg aggtcagcat cgggaactac gggaacaagt tcgacatgac ctgcctgccg
     3601 ctggcctcca ccactcagta cagccgtgca gtctttgacg ggtgccacta ctactaccta
     3661 ccctggggta acgtgaaacc tgtggtggtg ctgtcatcct actgggagga catcagccat
     3721 agaatcgaga ctcagaacca gctgcttggg attgctgacc ggctggaagc tggcctggag
     3781 caggtccacc tggccctgaa ggcgcagtgc tccacggagg acgtggactc gctggtggct
     3841 cagctgacgg atgagctcat cgcaggctgc agccagcctc tgggtgacat ccatgagaca
     3901 ccctctgcca cccacctgga ccagtacctg taccagctgc gcacccatca cctgagccaa
     3961 atcactgagg ctgccctggc cctgaagctc ggccacagtg agctccctgc agctctggag
     4021 caggcggagg actggctcct gcgtctgcgt gccctggcag aggagcccca gaacagcctg
     4081 ccggacatcg tcatctggat gctgcaggga gacaagcgtg tggcatacca gcgggtgccc
     4141 gcccaccaag tcctcttctc ccggcggggt gccaactact gtggcaagaa ttgtgggaag
     4201 ctacagacaa tctttctgaa atatccgatg gagaaggtgc ctggcgcccg gatgccagtg
     4261 cagatacggg tcaagctgtg gtttgggctc tctgtggatg agaaggagtt caaccagttt
     4321 gctgagggga agctgtctgt ctttgctgaa acctatgaga acgagactaa gttggccctt
     4381 gttgggaact ggggcacaac gggcctcacc taccccaagt tttctgacgt cacgggcaag
     4441 atcaagctac ccaaggacag cttccgcccc tcggccggct ggacctgggc tggagattgg
     4501 ttcgtgtgtc cggagaagac tctgctccat gacatggacg ccggtcacct gagcttcgtg
     4561 gaagaggtgt ttgagaacca gacccggctt cccggaggcc agtggatcta catgagtgac
     4621 aactacaccg atgtgaacgg ggagaaggtg cttcccaagg atgacattga gtgcccactg
     4681 ggctggaagt gggaagatga ggaatggtcc acagacctca accgggctgt cgatgagcaa
     4741 ggctgggagt atagcatcac catccccccg gagcggaagc cgaagcactg ggtccctgct
     4801 gagaagatgt actacacaca ccgacggcgg cgctgggtgc gcctgcgcag gagggatctc
     4861 agccaaatgg aagcactgaa aaggcacagg caggcggagg cggagggcga gggctgggag
     4921 tacgcctctc tttttggctg gaagttccac ctcgagtacc gcaagacaga tgccttccgc
     4981 cgccgccgct ggcgccgtcg catggagcca ctggagaaga cggggcctgc agctgtgttt
     5041 gcccttgagg gggccctggg cggcgtgatg gatgacaaga gtgaagattc catgtccgtc
     5101 tccaccttga gcttcggtgt gaacagaccc acgatttcct gcatattcga ctatgggaac
     5161 cgctaccatc tacgctgcta catgtaccag gcccgggacc tggctgcgat ggacaaggac
     5221 tctttttctg atccctatgc catcgtctcc ttcctgcacc agagccagaa gacggtggtg
     5281 gtgaagaaca cccttaaccc cacctgggac cagacgctca tcttctacga gatcgagatc
     5341 tttggcgagc cggccacagt tgctgagcaa ccgcccagca ttgtggtgga gctgtacgac
     5401 catgacactt atggtgcaga cgagtttatg ggtcgctgca tctgtcaacc gagtctggaa
     5461 cggatgccac ggctggcctg gttcccactg acgaggggca gccagccgtc gggggagctg
     5521 ctggcctctt ttgagctcat ccagagagag aagccggcca tccaccatat tcctggtttt
     5581 gaggtgcagg agacatcaag gatcctggat gagtctgagg acacagacct gccctaccca
     5641 ccaccccaga gggaggccaa catctacatg gttcctcaga acatcaagcc agcgctccag
     5701 cgtaccgcca tcgagatcct ggcatggggc ctgcggaaca tgaagagtta ccagctggcc
     5761 aacatctcct cccccagcct cgtggtagag tgtgggggcc agacggtgca gtcctgtgtc
     5821 atcaggaacc tccggaagaa ccccaacttt gacatctgca ccctcttcat ggaagtgatg
     5881 ctgcccaggg aggagctcta ctgccccccc atcaccgtca aggtcatcga taaccgccag
     5941 tttggccgcc ggcctgtggt gggccagtgt accatccgct ccctggagag cttcctgtgt
     6001 gacccctact cggcggagag tccatcccca cagggtggcc cagacgatgt gagcctactc
     6061 agtcctgggg aagacgtgct catcgacatt gatgacaagg agcccctcat ccccatccag
     6121 gaggaagagt tcatcgattg gtggagcaaa ttctttgcct ccatagggga gagggaaaag
     6181 tgcggctcct acctggagaa ggattttgac accctgaagg tctatgacac acagctggag
     6241 aatgtggagg cctttgaggg cctgtctgac ttttgtaaca ccttcaagct gtaccggggc
     6301 aagacgcagg aggagacaga agatccatct gtgattggtg aatttaaggg cctcttcaaa
     6361 atttatcccc tcccagaaga cccagccatc cccatgcccc caagacagtt ccaccagctg
     6421 gccgcccagg gaccccagga gtgcttggtc cgtatctaca ttgtccgagc atttggcctg
     6481 cagcccaagg accccaatgg aaagtgtgat ccttacatca agatctccat agggaagaaa
     6541 tcagtgagtg accaggataa ctacatcccc tgcacgctgg agcccgtatt tggaaagatg
     6601 ttcgagctga cctgcactct gcctctggag aaggacctaa agatcactct ctatgactat
     6661 gacctcctct ccaaggacga aaagatcggt gagacggtcg tcgacctgga gaacaggctg
     6721 ctgtccaagt ttggggctcg ctgtggactc ccacagacct actgtgtctc tggaccgaac
     6781 cagtggcggg accagctccg cccctcccag ctcctccacc tcttctgcca gcagcataga
     6841 gtcaaggcac ctgtgtaccg gacagaccgt gtaatgtttc aggataaaga atattccatt
     6901 gaagagatag aggctggcag gatcccaaac ccacacctgg gcccagtgga ggagcgtctg
     6961 gctctgcatg tgcttcagca gcagggcctg gtcccggagc acgtggagtc acggcccctc
     7021 tacagccccc tgcagccaga catcgagcag gggaagctgc agatgtgggt cgacctattt
     7081 ccgaaggccc tggggcggcc tggacctccc ttcaacatca ccccacggag agccagaagg
     7141 tttttcctgc gttgtattat ctggaatacc agagatgtga tcctggatga cctgagcctc
     7201 acgggggaga agatgagcga catttatgtg aaaggttgga tgattggctt tgaagaacac
     7261 aagcaaaaga cagacgtgca ttatcgttcc ctgggaggtg aaggcaactt caactggagg
     7321 ttcattttcc ccttcgacta cctgccagct gagcaagtct gtaccattgc caagaaggat
     7381 gccttctgga ggctggacaa gactgagagc aaaatcccag cacgagtggt gttccagatc
     7441 tgggacaatg acaagttctc ctttgatgat tttctgggct ccctgcagct cgatctcaac
     7501 cgcatgccca agccagccaa gacagccaag aagtgctcct tggaccagct ggatgatgct
     7561 ttccaccccg aatggtttgt gtcccttttt gagcagaaaa cagtgaaggg ctggtggccc
     7621 tgtgtagcag aagagggtga gaagaaaata ctggcgggca agctggaaat gaccttggag
     7681 attgtagcag agagtgagca tgaggagcgg cctgctggcc agggccggga tgagcccaac
     7741 atgaacccta agcttgagga cccaaggcgc cccgacacct ccttcctgtg gtttacctcc
     7801 ccatacaaga ccatgaagtt catcctgtgg cggcgtttcc ggtgactcga gtgagtgagc
     7861 ggccgcggat cccgggtggc atccctgtga cccctcccca gtgcctctcc tggccctgga
     7921 agttgccact ccagtgccca ccagccttgt cctaataaaa ttaagttgca tcattttgtc
     7981 tgactaggtg tccttctata atattatggg gtggaggggg gtggtatgga gcaaggggca
     8041 agttgggaag acaacctgta gggcctgcgg ggtctattgg gaaccaagct ggagtgcagt
     8101 ggcacaatct tggctcactg caatctccgc ctcctgggtt caagcgattc tcctgcctca
     8161 gcctcccgag ttgttgggat tccaggcatg catgaccagg ctcagctaat ttttgttttt
     8221 ttggtagaga cggggtttca ccatattggc caggctggtc tccaactcct aatctcaggt
     8281 gatctaccca ccttggcctc ccaaattgct gggattacag gcgtgaacca ctgctccctt
     8341 ccctgtcctt ctgattttaa aataactata ccagcaggag gacgtccaga cacagcatag
     8401 gctacctggc catgcccaac cggtgggaca tttgagttgc ttgcttggca ctgtcctctc
     8461 atgcgttggg tccactcagt agatgcctgt tgaattgggt acgcggccag cttggctgtg
     8521 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8581 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8641 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8701 gcccatcccg cctccgctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8761 aatttttttt atttatgcag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     8821 gaggaggctt ttttggagga attgatcagc ttgggatctg atcaagagac aggatgagga
     8881 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     8941 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     9001 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     9061 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     9121 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     9181 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     9241 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     9301 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     9361 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     9421 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     9481 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     9541 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     9601 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     9661 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     9721 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     9781 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     9841 agttcttcgc ccaccccggg ctcgatcccc tcgcgagttg gttcagctgc tgcctgaggc
     9901 tggacgacct cgcggagttc taccggcagt gcaaatccgt cggcatccag gaaaccagca
     9961 gcggctatcc gcgcatccat gcccccgaac tgcaggagtg gggaggcacg atggccgctt
    10021 tggtcgaccc ggacgggacg ctcctgcgcc tgatacagaa cgaattgctt gcaggcatct
    10081 catgagtgtg tcttcccgtt ttccgcctga ggtcactgcg tggatggagc gctggcgcct
    10141 gctgcgcgac ggcgagctgc tcaccaccca ctcgccaagc tggaaccgta aaaaggccgc
    10201 gttgctggcg tttttccata ggctccgccg atcataatca gccataccac atttgtagag
    10261 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
    10321 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10381 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10441 ctcatcaatg tatcttatca tgtctggatc aattccctat agtgagtcgt attaaattcg
    10501 taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
    10561 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
    10621 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
    10681 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
    10741 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
    10801 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
    10861 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
    10921 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
    10981 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
    11041 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
    11101 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
    11161 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
    11221 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
    11281 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
    11341 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
    11401 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
    11461 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
    11521 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    11581 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    11641 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    11701 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    11761 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    11821 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    11881 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    11941 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    12001 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    12061 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    12121 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    12181 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    12241 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    12301 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    12361 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    12421 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    12481 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    12541 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    12601 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    12661 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
    12721 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
    12781 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
    12841 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
    12901 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaagagtc
    12961 cactattaaa gaacgtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
    13021 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    13081 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa ttt
//
