LOCUS       sequence-135086-       34925 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  Express copGFP under EF-1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 34925)
  TITLE     plasmid for first generation adenovirus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 34925)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAdx-EF1-copGFP
FEATURES             Location/Qualifiers
     source          1..34925
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        133..1311
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          364..1302
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1259..1279
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1352..2014
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     polyA_signal    2122..2203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2159..2178)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2213..2232
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     misc_feature    join(5835..5900,5901..5938)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             complement(13032..13043)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      20515..20524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     repeat_region   31484..31586
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(31665..31684)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(31666..31684)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(31691..31708)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(31691..31707)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(31700..31722)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(31848..32303)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(31985..32006)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     32197..32216
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        32382..32486
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(32487..32555,32556..33347)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(32705..32724)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      33518..34106
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     34007..34026
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     34260..34277
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    34394..34415
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(34430..34435,34436..34453,34454..34460)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    34468..34484
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     34473..34495
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     34492..34508
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     34492..34508
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        34526..34544
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     34526..34543
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     repeat_region   34576..34678
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     34766..34916
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
ORIGIN
        1 cgtaactata acggtcctaa ggtagcgacg cgccctgcag tttcccaagc tttgcaaaga
       61 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
      121 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
      181 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
      241 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      301 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      361 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
      421 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
      481 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
      541 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
      601 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
      661 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
      721 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
      781 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
      841 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
      901 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
      961 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     1021 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     1081 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     1141 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     1201 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     1261 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattgga
     1321 tcccaccatg gagagcgacg agagcggcct gcccgccatg gagatcgagt gccgcatcac
     1381 cggcaccctg aacggcgtgg agttcgagct ggtgggcggc ggagagggca cccccaagca
     1441 gggccgcatg accaacaaga tgaagagcac caaaggcgcc ctgaccttca gcccctacct
     1501 gctgagccac gtgatgggct acggcttcta ccacttcggc acctacccca gcggctacga
     1561 gaaccccttc ctgcacgcca tcaacaacgg cggctacacc aacacccgca tcgagaagta
     1621 cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc tacgaggccg gccgcgtgat
     1681 cggcgacttc aaggtggtgg gcaccggctt ccccgaggac agcgtgatct tcaccgacaa
     1741 gatcatccgc agcaacgcca ccgtggagca cctgcacccc atgggcgata acgtgctggt
     1801 gggcagcttc gcccgcacct tcagcctgcg cgacggcggc tactacagct tcgtggtgga
     1861 cagccacatg cacttcaaga gcgccatcca ccccagcatc ctgcagaacg ggggccccat
     1921 gttcgccttc cgccgcgtgg aggagctgca cagcaacacc gagctgggca tcgtggagta
     1981 ccagcacgcc ttcaagaccc ccattgcctt cgccagatcc cgcgctcagt cgtccaattc
     2041 tgccgtggac ggcaccgccg gacccggctc caccggatct cgctaaggcg cgccgaattc
     2101 tatcgataag taagtgacta gaacttgttt attgcagctt ataatggtta caaataaagc
     2161 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     2221 tccaaactca tctgcacgcg tggcgcgtat ctatgtcggg tgcggagaaa gaggtaatga
     2281 aatggcaaaa ttatttaaat tcgactcgaa gatctgggcg tggttaaggg tgggaaagaa
     2341 tatataaggt gggggtctta tgtagttttg tatctgtttt gcagcagccg ccgccgccat
     2401 gagcaccaac tcgtttgatg gaagcattgt gagctcatat ttgacaacgc gcatgccccc
     2461 atgggccggg gtgcgtcaga atgtgatggg ctccagcatt gatggtcgcc ccgtcctgcc
     2521 cgcaaactct actaccttga cctacgagac cgtgtctgga acgccgttgg agactgcagc
     2581 ctccgccgcc gcttcagccg ctgcagccac cgcccgcggg attgtgactg actttgcttt
     2641 cctgagcccg cttgcaagca gtgcagcttc ccgttcatcc gcccgcgatg acaagttgac
     2701 ggctcttttg gcacaattgg attctttgac ccgggaactt aatgtcgttt ctcagcagct
     2761 gttggatctg cgccagcagg tttctgccct gaaggcttcc tcccctccca atgcggttta
     2821 aaacataaat aaaaaaccag actctgtttg gatttggatc aagcaagtgt cttgctgtct
     2881 ttatttaggg gttttgcgcg cgcggtaggc ccgggaccag cggtctcggt cgttgagggt
     2941 cctgtgtatt ttttccagga cgtggtaaag gtgactctgg atgttcagat acatgggcat
     3001 aagcccgtct ctggggtgga ggtagcacca ctgcagagct tcatgctgcg gggtggtgtt
     3061 gtagatgatc cagtcgtagc aggagcgctg ggcgtggtgc ctaaaaatgt ctttcagtag
     3121 caagctgatt gccaggggca ggcccttggt gtaagtgttt acaaagcggt taagctggga
     3181 tgggtgcata cgtggggata tgagatgcat cttggactgt atttttaggt tggctatgtt
     3241 cccagccata tccctccggg gattcatgtt gtgcagaacc accagcacag tgtatccggt
     3301 gcacttggga aatttgtcat gtagcttaga aggaaatgcg tggaagaact tggagacgcc
     3361 cttgtgacct ccaagatttt ccatgcattc gtccataatg atggcaatgg gcccacgggc
     3421 ggcggcctgg gcgaagatat ttctgggatc actaacgtca tagttgtgtt ccaggatgag
     3481 atcgtcatag gccattttta caaagcgcgg gcggagggtg ccagactgcg gtataatggt
     3541 tccatccggc ccaggggcgt agttaccctc acagatttgc atttcccacg ctttgagttc
     3601 agatgggggg atcatgtcta cctgcggggc gatgaagaaa acggtttccg gggtagggga
     3661 gatcagctgg gaagaaagca ggttcctgag cagctgcgac ttaccgcagc cggtgggccc
     3721 gtaaatcaca cctattaccg gctgcaactg gtagttaaga gagctgcagc tgccgtcatc
     3781 cctgagcagg ggggccactt cgttaagcat gtccctgact cgcatgtttt ccctgaccaa
     3841 atccgccaga aggcgctcgc cgcccagcga tagcagttct tgcaaggaag caaagttttt
     3901 caacggtttg agaccgtccg ccgtaggcat gcttttgagc gtttgaccaa gcagttccag
     3961 gcggtcccac agctcggtca cctgctctac ggcatctcga tccagcatat ctcctcgttt
     4021 cgcgggttgg ggcggctttc gctgtacggc agtagtcggt gctcgtccag acgggccagg
     4081 gtcatgtctt tccacgggcg cagggtcctc gtcagcgtag tctgggtcac ggtgaagggg
     4141 tgcgctccgg gctgcgcgct ggccagggtg cgcttgaggc tggtcctgct ggtgctgaag
     4201 cgctgccggt cttcgccctg cgcgtcggcc aggtagcatt tgaccatggt gtcatagtcc
     4261 agcccctccg cggcgtggcc cttggcgcgc agcttgccct tggaggaggc gccgcacgag
     4321 gggcagtgca gacttttgag ggcgtagagc ttgggcgcga gaaataccga ttccggggag
     4381 taggcatccg cgccgcaggc cccgcagacg gtctcgcatt ccacgagcca ggtgagctct
     4441 ggccgttcgg ggtcaaaaac caggtttccc ccatgctttt tgatgcgttt cttacctctg
     4501 gtttccatga gccggtgtcc acgctcggtg acgaaaaggc tgtccgtgtc cccgtataca
     4561 gacttgagag gcctgtcctc gagcggtgtt ccgcggtcct cctcgtatag aaactcggac
     4621 cactctgaga caaaggctcg cgtccaggcc agcacgaagg aggctaagtg ggaggggtag
     4681 cggtcgttgt ccactagggg gtccactcgc tccagggtgt gaagacacat gtcgccctct
     4741 tcggcatcaa ggaaggtgat tggtttgtag gtgtaggcca cgtgaccggg tgttcctgaa
     4801 ggggggctat aaaagggggt gggggcgcgt tcgtcctcac tctcttccgc atcgctgtct
     4861 gcgagggcca gctgttgggg tgagtactcc ctctgaaaag cgggcatgac ttctgcgcta
     4921 agattgtcag tttccaaaaa cgaggaggat ttgatattca cctggcccgc ggtgatgcct
     4981 ttgagggtgg ccgcatccat ctggtcagaa aagacaatct ttttgttgtc aagcttggtg
     5041 gcaaacgacc cgtagagggc gttggacagc aacttggcga tggagcgcag ggtttggttt
     5101 ttgtcgcgat cggcgcgctc cttggccgcg atgtttagct gcacgtattc gcgcgcaacg
     5161 caccgccatt cgggaaagac ggtggtgcgc tcgtcgggca ccaggtgcac gcgccaaccg
     5221 cggttgtgca gggtgacaag gtcaacgctg gtggctacct ctccgcgtag gcgctcgttg
     5281 gtccagcaga ggcggccgcc cttgcgcgag cagaatggcg gtagggggtc tagctgcgtc
     5341 tcgtccgggg ggtctgcgtc cacggtaaag accccgggca gcaggcgcgc gtcgaagtag
     5401 tctatcttgc atccttgcaa gtctagcgcc tgctgccatg cgcgggcggc aagcgcgcgc
     5461 tcgtatgggt tgagtggggg accccatggc atggggtggg tgagcgcgga ggcgtacatg
     5521 ccgcaaatgt cgtaaacgta gaggggctct ctgagtattc caagatatgt agggtagcat
     5581 cttccaccgc ggatgctggc gcgcacgtaa tcgtatagtt cgtgcgaggg agcgaggagg
     5641 tcgggaccga ggttgctacg ggcgggctgc tctgctcgga agactatctg cctgaagatg
     5701 gcatgtgagt tggatgatat ggttggacgc tggaagacgt tgaagctggc gtctgtgaga
     5761 cctaccgcgt cacgcacgaa ggaggcgtag gagtcgcgca gcttgttgac cagctcggcg
     5821 gtgacctgca cgtctagggc gcagtagtcc agggtttcct tgatgatgtc atacttatcc
     5881 tgtccctttt ttttccacag ctcgcggttg aggacaaact cttcgcggtc tttccagtac
     5941 tcttggatcg gaaacccgtc ggcctccgaa cggtaagagc ctagcatgta gaactggttg
     6001 acggcctggt aggcgcagca tcccttttct acgggtagcg cgtatgcctg cgcggccttc
     6061 cggagcgagg tgtgggtgag cgcaaaggtg tccctgacca tgactttgag gtactggtat
     6121 ttgaagtcag tgtcgtcgca tccgccctgc tcccagagca aaaagtccgt gcgctttttg
     6181 gaacgcggat ttggcagggc gaaggtgaca tcgttgaaga gtatctttcc cgcgcgaggc
     6241 ataaagttgc gtgtgatgcg gaagggtccc ggcacctcgg aacggttgtt aattacctgg
     6301 gcggcgagca cgatctcgtc aaagccgttg atgttgtggc ccacaatgta aagttccaag
     6361 aagcgcggga tgcccttgat ggaaggcaat tttttaagtt cctcgtaggt gagctcttca
     6421 ggggagctga gcccgtgctc tgaaagggcc cagtctgcaa gatgagggtt ggaagcgacg
     6481 aatgagctcc acaggtcacg ggccattagc atttgcaggt ggtcgcgaaa ggtcctaaac
     6541 tggcgaccta tggccatttt ttctggggtg atgcagtaga aggtaagcgg gtcttgttcc
     6601 cagcggtccc atccaaggtt cgcggctagg tctcgcgcgg cagtcactag aggctcatct
     6661 ccgccgaact tcatgaccag catgaagggc acgagctgct tcccaaaggc ccccatccaa
     6721 gtataggtct ctacatcgta ggtgacaaag agacgctcgg tgcgaggatg cgagccgatc
     6781 gggaagaact ggatctcccg ccaccaattg gaggagtggc tattgatgtg gtgaaagtag
     6841 aagtccctgc gacgggccga acactcgtgc tggcttttgt aaaaacgtgc gcagtactgg
     6901 cagcggtgca cgggctgtac atcctgcacg aggttgacct gacgaccgcg cacaaggaag
     6961 cagagtggga atttgagccc ctcgcctggc gggtttggct ggtggtcttc tacttcggct
     7021 gcttgtcctt gaccgtctgg ctgctcgagg ggagttacgg tggatcggac caccacgccg
     7081 cgcgagccca aagtccagat gtccgcgcgc ggcggtcgga gcttgatgac aacatcgcgc
     7141 agatgggagc tgtccatggt ctggagctcc cgcggcgtca ggtcaggcgg gagctcctgc
     7201 aggtttacct cgcatagacg ggtcagggcg cgggctagat ccaggtgata cctaatttcc
     7261 aggggctggt tggtggcggc gtcgatggct tgcaagaggc cgcatccccg cggcgcgact
     7321 acggtaccgc gcggcgggcg gtgggccgcg ggggtgtcct tggatgatgc atctaaaagc
     7381 ggtgacgcgg gcgagccccc ggaggtaggg ggggctccgg acccgccggg agagggggca
     7441 ggggcacgtc ggcgccgcgc gcgggcagga gctggtgctg cgcgcgtagg ttgctggcga
     7501 acgcgacgac gcggcggttg atctcctgaa tctggcgcct ctgcgtgaag acgacgggcc
     7561 cggtgagctt gaacctgaaa gagagttcga cagaatcaat ttcggtgtcg ttgacggcgg
     7621 cctggcgcaa aatctcctgc acgtctcctg agttgtcttg ataggcgatc tcggccatga
     7681 actgctcgat ctcttcctcc tggagatctc cgcgtccggc tcgctccacg gtggcggcga
     7741 ggtcgttgga aatgcgggcc atgagctgcg agaaggcgtt gaggcctccc tcgttccaga
     7801 cgcggctgta gaccacgccc ccttcggcat cgcgggcgcg catgaccacc tgcgcgagat
     7861 tgagctccac gtgccgggcg aagacggcgt agtttcgcag gcgctgaaag aggtagttga
     7921 gggtggtggc ggtgtgttct gccacgaaga agtacataac ccagcgtcgc aacgtggatt
     7981 cgttgatatc ccccaaggcc tcaaggcgct ccatggcctc gtagaagtcc acggcgaagt
     8041 tgaaaaactg ggagttgcgc gccgacacgg ttaactcctc ctccagaaga cggatgagct
     8101 cggcgacagt gtcgcgcacc tcgcgctcaa aggctacagg ggcctcttct tcttcttcaa
     8161 tctcctcttc cataagggcc tccccttctt cttcttctgg cggcggtggg ggagggggga
     8221 cacggcggcg acgacggcgc accgggaggc ggtcgacaaa gcgctcgatc atctccccgc
     8281 ggcgacggcg catggtctcg gtgacggcgc ggccgttctc gcgggggcgc agttggaaga
     8341 cgccgcccgt catgtcccgg ttatgggttg gcggggggct gccatgcggc agggatacgg
     8401 cgctaacgat gcatctcaac aattgttgtg taggtactcc gccgccgagg gacctgagcg
     8461 agtccgcatc gaccggatcg gaaaacctct cgagaaaggc gtctaaccag tcacagtcgc
     8521 aaggtaggct gagcaccgtg gcgggcggca gcgggcggcg gtcggggttg tttctggcgg
     8581 aggtgctgct gatgatgtaa ttaaagtagg cggtcttgag acggcggatg gtcgacagaa
     8641 gcaccatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg ccccaggctt
     8701 cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct accggcactt
     8761 cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctgcggcg gcggcggagt
     8821 ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc ctcatcggct
     8881 gaagcagggc taggtcggcg acaacgcgct cggctaatat ggcctgctgc acctgcgtga
     8941 gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg ttgatggtgt
     9001 aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc gagagctcgg
     9061 tgtacctgag acgcgagtaa gccctcgagt caaatacgta gtcgttgcaa gtccgcacca
     9121 ggtactggta tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc cagcgtaggg
     9181 tggccggggc tccgggggcg agatcttcca acataaggcg atgatatccg tagatgtacc
     9241 tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg cggacgcggt
     9301 tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg ccggtcaggc
     9361 gcgcgcaatc gttgacgctc tagcgtgcaa aaggagagcc tgtaagcggg cactcttccg
     9421 tggtctggtg gataaattcg caagggtatc atggcggacg accggggttc gagccccgta
     9481 tccggccgtc cgccgtgatc catgcggtta ccgcccgcgt gtcgaaccca ggtgtgcgac
     9541 gtcagacaac gggggagtgc tccttttggc ttccttccag gcgcggcggc tgctgcgcta
     9601 gcttttttgg ccactggccg cgcgcagcgt aagcggttag gctggaaagc gaaagcatta
     9661 agtggctcgc tccctgtagc cggagggtta ttttccaagg gttgagtcgc gggacccccg
     9721 gttcgagtct cggaccggcc ggactgcggc gaacgggggt ttgcctcccc gtcatgcaag
     9781 accccgcttg caaattcctc cggaaacagg gacgagcccc ttttttgctt ttcccagatg
     9841 catccggtgc tgcggcagat gcgcccccct cctcagcagc ggcaagagca agagcagcgg
     9901 cagacatgca gggcaccctc ccctcctcct accgcgtcag gaggggcgac atccgcggtt
     9961 gacgcggcag cagatggtga ttacgaaccc ccgcggcgcc gggcccggca ctacctggac
    10021 ttggaggagg gcgagggcct ggcgcggcta ggagcgccct ctcctgagcg gcacccaagg
    10081 gtgcagctga agcgtgatac gcgtgaggcg tacgtgccgc ggcagaacct gtttcgcgac
    10141 cgcgagggag aggagcccga ggagatgcgg gatcgaaagt tccacgcagg gcgcgagctg
    10201 cggcatggcc tgaatcgcga gcggttgctg cgcgaggagg actttgagcc cgacgcgcga
    10261 accgggatta gtcccgcgcg cgcacacgtg gcggccgccg acctggtaac cgcatacgag
    10321 cagacggtga accaggagat taactttcaa aaaagcttta acaaccacgt gcgtacgctt
    10381 gtggcgcgcg aggaggtggc tataggactg atgcatctgt gggactttgt aagcgcgctg
    10441 gagcaaaacc caaatagcaa gccgctcatg gcgcagctgt tccttatagt gcagcacagc
    10501 agggacaacg aggcattcag ggatgcgctg ctaaacatag tagagcccga gggccgctgg
    10561 ctgctcgatt tgataaacat cctgcagagc atagtggtgc aggagcgcag cttgagcctg
    10621 gctgacaagg tggccgccat caactattcc atgcttagcc tgggcaagtt ttacgcccgc
    10681 aagatatacc atacccctta cgttcccata gacaaggagg taaagatcga ggggttctac
    10741 atgcgcatgg cgctgaaggt gcttaccttg agcgacgacc tgggcgttta tcgcaacgag
    10801 cgcatccaca aggccgtgag cgtgagccgg cggcgcgagc tcagcgaccg cgagctgatg
    10861 cacagcctgc aaagggccct ggctggcacg ggcagcggcg atagagaggc cgagtcctac
    10921 tttgacgcgg gcgctgacct gcgctgggcc ccaagccgac gcgccctgga ggcagctggg
    10981 gccggacctg ggctggcggt ggcacccgcg cgcgctggca acgtcggcgg cgtggaggaa
    11041 tatgacgagg acgatgagta cgagccagag gacggcgagt actaagcggt gatgtttctg
    11101 atcagatgat gcaagacgca acggacccgg cggtgcgggc ggcgctgcag agccagccgt
    11161 ccggccttaa ctccacggac gactggcgcc aggtcatgga ccgcatcatg tcgctgactg
    11221 cgcgcaatcc tgacgcgttc cggcagcagc cgcaggccaa ccggctctcc gcaattctgg
    11281 aagcggtggt cccggcgcgc gcaaacccca cgcacgagaa ggtgctggcg atcgtaaacg
    11341 cgctggccga aaacagggcc atccggcccg acgaggccgg cctggtctac gacgcgctgc
    11401 ttcagcgcgt ggctcgttac aacagcggca acgtgcagac caacctggac cggctggtgg
    11461 gggatgtgcg cgaggccgtg gcgcagcgtg agcgcgcgca gcagcagggc aacctgggct
    11521 ccatggttgc actaaacgcc ttcctgagta cacagcccgc caacgtgccg cggggacagg
    11581 aggactacac caactttgtg agcgcactgc ggctaatggt gactgagaca ccgcaaagtg
    11641 aggtgtacca gtctgggcca gactattttt tccagaccag tagacaaggc ctgcagaccg
    11701 taaacctgag ccaggctttc aaaaacttgc aggggctgtg gggggtgcgg gctcccacag
    11761 gcgaccgcgc gaccgtgtct agcttgctga cgcccaactc gcgcctgttg ctgctgctaa
    11821 tagcgccctt cacggacagt ggcagcgtgt cccgggacac atacctaggt cacttgctga
    11881 cactgtaccg cgaggccata ggtcaggcgc atgtggacga gcatactttc caggagatta
    11941 caagtgtcag ccgcgcgctg gggcaggagg acacgggcag cctggaggca accctaaact
    12001 acctgctgac caaccggcgg cagaagatcc cctcgttgca cagtttaaac agcgaggagg
    12061 agcgcatttt gcgctacgtg cagcagagcg tgagccttaa cctgatgcgc gacggggtaa
    12121 cgcccagcgt ggcgctggac atgaccgcgc gcaacatgga accgggcatg tatgcctcaa
    12181 accggccgtt tatcaaccgc ctaatggact acttgcatcg cgcggccgcc gtgaaccccg
    12241 agtatttcac caatgccatc ttgaacccgc actggctacc gccccctggt ttctacaccg
    12301 ggggattcga ggtgcccgag ggtaacgatg gattcctctg ggacgacata gacgacagcg
    12361 tgttttcccc gcaaccgcag accctgctag agttgcaaca gcgcgagcag gcagaggcgg
    12421 cgctgcgaaa ggaaagcttc cgcaggccaa gcagcttgtc cgatctaggc gctgcggccc
    12481 cgcggtcaga tgctagtagc ccatttccaa gcttgatagg gtctcttacc agcactcgca
    12541 ccacccgccc gcgcctgctg ggcgaggagg agtacctaaa caactcgctg ctgcagccgc
    12601 agcgcgaaaa aaacctgcct ccggcatttc ccaacaacgg gatagagagc ctagtggaca
    12661 agatgagtag atggaagacg tacgcgcagg agcacaggga cgtgccaggc ccgcgcccgc
    12721 ccacccgtcg tcaaaggcac gaccgtcagc ggggtctggt gtgggaggac gatgactcgg
    12781 cagacgacag cagcgtcctg gatttgggag ggagtggcaa cccgtttgcg caccttcgcc
    12841 ccaggctggg gagaatgttt taaaaaaaaa aaaagcatga tgcaaaataa aaaactcacc
    12901 aaggccatgg caccgagcgt tggttttctt gtattcccct tagtatgcgg cgcgcggcga
    12961 tgtatgagga aggtcctcct ccctcctacg agagtgtggt gagcgcggcg ccagtggcgg
    13021 cggcgctggg ttctcccttc gatgctcccc tggacccgcc gtttgtgcct ccgcggtacc
    13081 tgcggcctac cggggggaga aacagcatcc gttactctga gttggcaccc ctattcgaca
    13141 ccacccgtgt gtacctggtg gacaacaagt caacggatgt ggcatccctg aactaccaga
    13201 acgaccacag caactttctg accacggtca ttcaaaacaa tgactacagc ccgggggagg
    13261 caagcacaca gaccatcaat cttgacgacc ggtcgcactg gggcggcgac ctgaaaacca
    13321 tcctgcatac caacatgcca aatgtgaacg agttcatgtt taccaataag tttaaggcgc
    13381 gggtgatggt gtcgcgcttg cctactaagg acaatcaggt ggagctgaaa tacgagtggg
    13441 tggagttcac gctgcccgag ggcaactact ccgagaccat gaccatagac cttatgaaca
    13501 acgcgatcgt ggagcactac ttgaaagtgg gcagacagaa cggggttctg gaaagcgaca
    13561 tcggggtaaa gtttgacacc cgcaacttca gactggggtt tgaccccgtc actggtcttg
    13621 tcatgcctgg ggtatataca aacgaagcct tccatccaga catcattttg ctgccaggat
    13681 gcggggtgga cttcacccac agccgcctga gcaacttgtt gggcatccgc aagcggcaac
    13741 ccttccagga gggctttagg atcacctacg atgatctgga gggtggtaac attcccgcac
    13801 tgttggatgt ggacgcctac caggcgagct tgaaagatga caccgaacag ggcgggggtg
    13861 gcgcaggcgg cagcaacagc agtggcagcg gcgcggaaga gaactccaac gcggcagccg
    13921 cggcaatgca gccggtggag gacatgaacg atcatgccat tcgcggcgac acctttgcca
    13981 cacgggctga ggagaagcgc gctgaggccg aagcagcggc cgaagctgcc gcccccgctg
    14041 cgcaacccga ggtcgagaag cctcagaaga aaccggtgat caaacccctg acagaggaca
    14101 gcaagaaacg cagttacaac ctaataagca atgacagcac cttcacccag taccgcagct
    14161 ggtaccttgc atacaactac ggcgaccctc agaccggaat ccgctcatgg accctgcttt
    14221 gcactcctga cgtaacctgc ggctcggagc aggtctactg gtcgttgcca gacatgatgc
    14281 aagaccccgt gaccttccgc tccacgcgcc agatcagcaa ctttccggtg gtgggcgccg
    14341 agctgttgcc cgtgcactcc aagagcttct acaacgacca ggccgtctac tcccaactca
    14401 tccgccagtt tacctctctg acccacgtgt tcaatcgctt tcccgagaac cagattttgg
    14461 cgcgcccgcc agcccccacc atcaccaccg tcagtgaaaa cgttcctgct ctcacagatc
    14521 acgggacgct accgctgcgc aacagcatcg gaggagtcca gcgagtgacc attactgacg
    14581 ccagacgccg cacctgcccc tacgtttaca aggccctggg catagtctcg ccgcgcgtcc
    14641 tatcgagccg cactttttga gcaagcatgt ccatccttat atcgcccagc aataacacag
    14701 gctggggcct gcgcttccca agcaagatgt ttggcggggc caagaagcgc tccgaccaac
    14761 acccagtgcg cgtgcgcggg cactaccgcg cgccctgggg cgcgcacaaa cgcggccgca
    14821 ctgggcgcac caccgtcgat gacgccatcg acgcggtggt ggaggaggcg cgcaactaca
    14881 cgcccacgcc gccaccagtg tccacagtgg acgcggccat tcagaccgtg gtgcgcggag
    14941 cccggcgcta tgctaaaatg aagagacggc ggaggcgcgt agcacgtcgc caccgccgcc
    15001 gacccggcac tgccgcccaa cgcgcggcgg cggccctgct taaccgcgca cgtcgcaccg
    15061 gccgacgggc ggccatgcgg gccgctcgaa ggctggccgc gggtattgtc actgtgcccc
    15121 ccaggtccag gcgacgagcg gccgccgcag cagccgcggc cattagtgct atgactcagg
    15181 gtcgcagggg caacgtgtat tgggtgcgcg actcggttag cggcctgcgc gtgcccgtgc
    15241 gcacccgccc cccgcgcaac tagattgcaa gaaaaaacta cttagactcg tactgttgta
    15301 tgtatccagc ggcggcggcg cgcaacgaag ctatgtccaa gcgcaaaatc aaagaagaga
    15361 tgctccaggt catcgcgccg gagatctatg gccccccgaa gaaggaagag caggattaca
    15421 agccccgaaa gctaaagcgg gtcaaaaaga aaaagaaaga tgatgatgat gaacttgacg
    15481 acgaggtgga actgctgcac gctaccgcgc ccaggcgacg ggtacagtgg aaaggtcgac
    15541 gcgtaaaacg tgttttgcga cccggcacca ccgtagtctt tacgcccggt gagcgctcca
    15601 cccgcaccta caagcgcgtg tatgatgagg tgtacggcga cgaggacctg cttgagcagg
    15661 ccaacgagcg cctcggggag tttgcctacg gaaagcggca taaggacatg ctggcgttgc
    15721 cgctggacga gggcaaccca acacctagcc taaagcccgt aacactgcag caggtgctgc
    15781 ccgcgcttgc accgtccgaa gaaaagcgcg gcctaaagcg cgagtctggt gacttggcac
    15841 ccaccgtgca gctgatggta cccaagcgcc agcgactgga agatgtcttg gaaaaaatga
    15901 ccgtggaacc tgggctggag cccgaggtcc gcgtgcggcc aatcaagcag gtggcgccgg
    15961 gactgggcgt gcagaccgtg gacgttcaga tacccactac cagtagcacc agtattgcca
    16021 ccgccacaga gggcatggag acacaaacgt ccccggttgc ctcagcggtg gcggatgccg
    16081 cggtgcaggc ggtcgctgcg gccgcgtcca agacctctac ggaggtgcaa acggacccgt
    16141 ggatgtttcg cgtttcagcc ccccggcgcc cgcgccgttc gaggaagtac ggcgccgcca
    16201 gcgcgctact gcccgaatat gccctacatc cttccattgc gcctaccccc ggctatcgtg
    16261 gctacaccta ccgccccaga agacgagcaa ctacccgacg ccgaaccacc actggaaccc
    16321 gccgccgccg tcgccgtcgc cagcccgtgc tggccccgat ttccgtgcgc agggtggctc
    16381 gcgaaggagg caggaccctg gtgctgccaa cagcgcgcta ccaccccagc atcgtttaaa
    16441 agccggtctt tgtggttctt gcagatatgg ccctcacctg ccgcctccgt ttcccggtgc
    16501 cgggattccg aggaagaatg caccgtagga ggggcatggc cggccacggc ctgacgggcg
    16561 gcatgcgtcg tgcgcaccac cggcggcggc gcgcgtcgca ccgtcgcatg cgcggcggta
    16621 tcctgcccct ccttattcca ctgatcgccg cggcgattgg cgccgtgccc ggaattgcat
    16681 ccgtggcctt gcaggcgcag agacactgat taaaaacaag ttgcatgtgg aaaaatcaaa
    16741 ataaaaagtc tggactctca cgctcgcttg gtcctgtaac tattttgtag aatggaagac
    16801 atcaactttg cgtctctggc cccgcgacac ggctcgcgcc cgttcatggg aaactggcaa
    16861 gatatcggca ccagcaatat gagcggtggc gccttcagct ggggctcgct gtggagcggc
    16921 attaaaaatt tcggttccac cgttaagaac tatggcagca aggcctggaa cagcagcaca
    16981 ggccagatgc tgagggataa gttgaaagag caaaatttcc aacaaaaggt ggtagatggc
    17041 ctggcctctg gcattagcgg ggtggtggac ctggccaacc aggcagtgca aaataagatt
    17101 aacagtaagc ttgatccccg ccctcccgta gaggagcctc caccggccgt ggagacagtg
    17161 tctccagagg ggcgtggcga aaagcgtccg cgccccgaca gggaagaaac tctggtgacg
    17221 caaatagacg agcctccctc gtacgaggag gcactaaagc aaggcctgcc caccacccgt
    17281 cccatcgcgc ccatggctac cggagtgctg ggccagcaca cacccgtaac gctggacctg
    17341 cctccccccg ccgacaccca gcagaaacct gtgctgccag gcccgaccgc cgttgttgta
    17401 acccgtccta gccgcgcgtc cctgcgccgc gccgccagcg gtccgcgatc gttgcggccc
    17461 gtagccagtg gcaactggca aagcacactg aacagcatcg tgggtctggg ggtgcaatcc
    17521 ctgaagcgcc gacgatgctt ctgatagcta acgtgtcgta tgtgtgtcat gtatgcgtcc
    17581 atgtcgccgc cagaggagct gctgagccgc cgcgcgcccg ctttccaaga tggctacccc
    17641 ttcgatgatg ccgcagtggt cttacatgca catctcgggc caggacgcct cggagtacct
    17701 gagccccggg ctggtgcagt ttgcccgcgc caccgagacg tacttcagcc tgaataacaa
    17761 gtttagaaac cccacggtgg cgcctacgca cgacgtgacc acagaccggt cccagcgttt
    17821 gacgctgcgg ttcatccctg tggaccgtga ggatactgcg tactcgtaca aggcgcggtt
    17881 caccctagct gtgggtgata accgtgtgct ggacatggct tccacgtact ttgacatccg
    17941 cggcgtgctg gacaggggcc ctacttttaa gccctactct ggcactgcct acaacgccct
    18001 ggctcccaag ggtgccccaa atccttgcga atgggatgaa gctgctactg ctcttgaaat
    18061 aaacctagaa gaagaggacg atgacaacga agacgaagta gacgagcaag ctgagcagca
    18121 aaaaactcac gtatttgggc aggcgcctta ttctggtata aatattacaa aggagggtat
    18181 tcaaataggt gtcgaaggtc aaacacctaa atatgccgat aaaacatttc aacctgaacc
    18241 tcaaatagga gaatctcagt ggtacgaaac agaaattaat catgcagctg ggagagtcct
    18301 aaaaaagact accccaatga aaccatgtta cggttcatat gcaaaaccca caaatgaaaa
    18361 tggagggcaa ggcattcttg taaagcaaca aaatggaaag ctagaaagtc aagtggaaat
    18421 gcaatttttc tcaactactg aggcagccgc aggcaatggt gataacttga ctcctaaagt
    18481 ggtattgtac agtgaagatg tagatataga aaccccagac actcatattt cttacatgcc
    18541 cactattaag gaaggtaact cacgagaact aatgggccaa caatctatgc ccaacaggcc
    18601 taattacatt gcttttaggg acaattttat tggtctaatg tattacaaca gcacgggtaa
    18661 tatgggtgtt ctggcgggcc aagcatcgca gttgaatgct gttgtagatt tgcaagacag
    18721 aaacacagag ctttcatacc agcttttgct tgattccatt ggtgatagaa ccaggtactt
    18781 ttctatgtgg aatcaggctg ttgacagcta tgatccagat gttagaatta ttgaaaatca
    18841 tggaactgaa gatgaacttc caaattactg ctttccactg ggaggtgtga ttaatacaga
    18901 gactcttacc aaggtaaaac ctaaaacagg tcaggaaaat ggatgggaaa aagatgctac
    18961 agaattttca gataaaaatg aaataagagt tggaaataat tttgccatgg aaatcaatct
    19021 aaatgccaac ctgtggagaa atttcctgta ctccaacata gcgctgtatt tgcccgacaa
    19081 gctaaagtac agtccttcca acgtaaaaat ttctgataac ccaaacacct acgactacat
    19141 gaacaagcga gtggtggctc ccgggctagt ggactgctac attaaccttg gagcacgctg
    19201 gtcccttgac tatatggaca acgtcaaccc atttaaccac caccgcaatg ctggcctgcg
    19261 ctaccgctca atgttgctgg gcaatggtcg ctatgtgccc ttccacatcc aggtgcctca
    19321 gaagttcttt gccattaaaa acctccttct cctgccgggc tcatacacct acgagtggaa
    19381 cttcaggaag gatgttaaca tggttctgca gagctcccta ggaaatgacc taagggttga
    19441 cggagccagc attaagtttg atagcatttg cctttacgcc accttcttcc ccatggccca
    19501 caacaccgcc tccacgcttg aggccatgct tagaaacgac accaacgacc agtcctttaa
    19561 cgactatctc tccgccgcca acatgctcta ccctataccc gccaacgcta ccaacgtgcc
    19621 catatccatc ccctcccgca actgggcggc tttccgcggc tgggccttca cgcgccttaa
    19681 gactaaggaa accccatcac tgggctcggg ctacgaccct tattacacct actctggctc
    19741 tataccctac ctagatggaa ccttttacct caaccacacc tttaagaagg tggccattac
    19801 ctttgactct tctgtcagct ggcctggcaa tgaccgcctg cttaccccca acgagtttga
    19861 aattaagcgc tcagttgacg gggagggtta caacgttgcc cagtgtaaca tgaccaaaga
    19921 ctggttcctg gtacaaatgc tagctaacta taacattggc taccagggct tctatatccc
    19981 agagagctac aaggaccgca tgtactcctt ctttagaaac ttccagccca tgagccgtca
    20041 ggtggtggat gatactaaat acaaggacta ccaacaggtg ggcatcctac accaacacaa
    20101 caactctgga tttgttggct accttgcccc caccatgcgc gaaggacagg cctaccctgc
    20161 taacttcccc tatccgctta taggcaagac cgcagttgac agcattaccc agaaaaagtt
    20221 tctttgcgat cgcacccttt ggcgcatccc attctccagt aactttatgt ccatgggcgc
    20281 actcacagac ctgggccaaa accttctcta cgccaactcc gcccacgcgc tagacatgac
    20341 ttttgaggtg gatcccatgg acgagcccac ccttctttat gttttgtttg aagtctttga
    20401 cgtggtccgt gtgcaccagc cgcaccgcgg cgtcatcgaa accgtgtacc tgcgcacgcc
    20461 cttctcggcc ggcaacgcca caacataaag aagcaagcaa catcaacaac agctgccgcc
    20521 atgggctcca gtgagcagga actgaaagcc attgtcaaag atcttggttg tgggccatat
    20581 tttttgggca cctatgacaa gcgctttcca ggctttgttt ctccacacaa gctcgcctgc
    20641 gccatagtca atacggccgg tcgcgagact gggggcgtac actggatggc ctttgcctgg
    20701 aacccgcact caaaaacatg ctacctcttt gagccctttg gcttttctga ccagcgactc
    20761 aagcaggttt accagtttga gtacgagtca ctcctgcgcc gtagcgccat tgcttcttcc
    20821 cccgaccgct gtataacgct ggaaaagtcc acccaaagcg tacaggggcc caactcggcc
    20881 gcctgtggac tattctgctg catgtttctc cacgcctttg ccaactggcc ccaaactccc
    20941 atggatcaca accccaccat gaaccttatt accggggtac ccaactccat gctcaacagt
    21001 ccccaggtac agcccaccct gcgtcgcaac caggaacagc tctacagctt cctggagcgc
    21061 cactcgccct acttccgcag ccacagtgcg cagattagga gcgccacttc tttttgtcac
    21121 ttgaaaaaca tgtaaaaata atgtactaga gacactttca ataaaggcaa atgcttttat
    21181 ttgtacactc tcgggtgatt atttaccccc acccttgccg tctgcgccgt ttaaaaatca
    21241 aaggggttct gccgcgcatc gctatgcgcc actggcaggg acacgttgcg atactggtgt
    21301 ttagtgctcc acttaaactc aggcacaacc atccgcggca gctcggtgaa gttttcactc
    21361 cacaggctgc gcaccatcac caacgcgttt agcaggtcgg gcgccgatat cttgaagtcg
    21421 cagttggggc ctccgccctg cgcgcgcgag ttgcgataca cagggttgca gcactggaac
    21481 actatcagcg ccgggtggtg cacgctggcc agcacgctct tgtcggagat cagatccgcg
    21541 tccaggtcct ccgcgttgct cagggcgaac ggagtcaact ttggtagctg ccttcccaaa
    21601 aagggcgcgt gcccaggctt tgagttgcac tcgcaccgta gtggcatcaa aaggtgaccg
    21661 tgcccggtct gggcgttagg atacagcgcc tgcataaaag ccttgatctg cttaaaagcc
    21721 acctgagcct ttgcgccttc agagaagaac atgccgcaag acttgccgga aaactgattg
    21781 gccggacagg ccgcgtcgtg cacgcagcac cttgcgtcgg tgttggagat ctgcaccaca
    21841 tttcggcccc accggttctt cacgatcttg gccttgctag actgctcctt cagcgcgcgc
    21901 tgcccgtttt cgctcgtcac atccatttca atcacgtgct ccttatttat cataatgctt
    21961 ccgtgtagac acttaagctc gccttcgatc tcagcgcagc ggtgcagcca caacgcgcag
    22021 cccgtgggct cgtgatgctt gtaggtcacc tctgcaaacg actgcaggta cgcctgcagg
    22081 aatcgcccca tcatcgtcac aaaggtcttg ttgctggtga aggtcagctg caacccgcgg
    22141 tgctcctcgt tcagccaggt cttgcatacg gccgccagag cttccacttg gtcaggcagt
    22201 agtttgaagt tcgcctttag atcgttatcc acgtggtact tgtccatcag cgcgcgcgca
    22261 gcctccatgc ccttctccca cgcagacacg atcggcacac tcagcgggtt catcaccgta
    22321 atttcacttt ccgcttcgct gggctcttcc tcttcctctt gcgtccgcat accacgcgcc
    22381 actgggtcgt cttcattcag ccgccgcact gtgcgcttac ctcctttgcc atgcttgatt
    22441 agcaccggtg ggttgctgaa acccaccatt tgtagcgcca catcttctct ttcttcctcg
    22501 ctgtccacga ttacctctgg tgatggcggg cgctcgggct tgggagaagg gcgcttcttt
    22561 ttcttcttgg gcgcaatggc caaatccgcc gccgaggtcg atggccgcgg gctgggtgtg
    22621 cgcggcacca gcgcgtcttg tgatgagtct tcctcgtcct cggactcgat acgccgcctc
    22681 atccgctttt ttgggggcgc ccggggaggc ggcggcgacg gggacgggga cgacacgtcc
    22741 tccatggttg ggggacgtcg cgccgcaccg cgtccgcgct cgggggtggt ttcgcgctgc
    22801 tcctcttccc gactggccat ttccttctcc tataggcaga aaaagatcat ggagtcagtc
    22861 gagaagaagg acagcctaac cgccccctct gagttcgcca ccaccgcctc caccgatgcc
    22921 gccaacgcgc ctaccacctt ccccgtcgag gcacccccgc ttgaggagga ggaagtgatt
    22981 atcgagcagg acccaggttt tgtaagcgaa gacgacgagg accgctcagt accaacagag
    23041 gataaaaagc aagaccagga caacgcagag gcaaacgagg aacaagtcgg gcggggggac
    23101 gaaaggcatg gcgactacct agatgtggga gacgacgtgc tgttgaagca tctgcagcgc
    23161 cagtgcgcca ttatctgcga cgcgttgcaa gagcgcagcg atgtgcccct cgccatagcg
    23221 gatgtcagcc ttgcctacga acgccaccta ttctcaccgc gcgtaccccc caaacgccaa
    23281 gaaaacggca catgcgagcc caacccgcgc ctcaacttct accccgtatt tgccgtgcca
    23341 gaggtgcttg ccacctatca catctttttc caaaactgca agatacccct atcctgccgt
    23401 gccaaccgca gccgagcgga caagcagctg gccttgcggc agggcgctgt catacctgat
    23461 atcgcctcgc tcaacgaagt gccaaaaatc tttgagggtc ttggacgcga cgagaagcgc
    23521 gcggcaaacg ctctgcaaca ggaaaacagc gaaaatgaaa gtcactctgg agtgttggtg
    23581 gaactcgagg gtgacaacgc gcgcctagcc gtactaaaac gcagcatcga ggtcacccac
    23641 tttgcctacc cggcacttaa cctacccccc aaggtcatga gcacagtcat gagtgagctg
    23701 atcgtgcgcc gtgcgcagcc cctggagagg gatgcaaatt tgcaagaaca aacagaggag
    23761 ggcctacccg cagttggcga cgagcagcta gcgcgctggc ttcaaacgcg cgagcctgcc
    23821 gacttggagg agcgacgcaa actaatgatg gccgcagtgc tcgttaccgt ggagcttgag
    23881 tgcatgcagc ggttctttgc tgacccggag atgcagcgca agctagagga aacattgcac
    23941 tacacctttc gacagggcta cgtacgccag gcctgcaaga tctccaacgt ggagctctgc
    24001 aacctggtct cctaccttgg aattttgcac gaaaaccgcc ttgggcaaaa cgtgcttcat
    24061 tccacgctca agggcgaggc gcgccgcgac tacgtccgcg actgcgttta cttatttcta
    24121 tgctacacct ggcagacggc catgggcgtt tggcagcagt gcttggagga gtgcaacctc
    24181 aaggagctgc agaaactgct aaagcaaaac ttgaaggacc tatggacggc cttcaacgag
    24241 cgctccgtgg ccgcgcacct ggcggacatc attttccccg aacgcctgct taaaaccctg
    24301 caacagggtc tgccagactt caccagtcaa agcatgttgc agaactttag gaactttatc
    24361 ctagagcgct caggaatctt gcccgccacc tgctgtgcac ttcctagcga ctttgtgccc
    24421 attaagtacc gcgaatgccc tccgccgctt tggggccact gctaccttct gcagctagcc
    24481 aactaccttg cctaccactc tgacataatg gaagacgtga gcggtgacgg tctactggag
    24541 tgtcactgtc gctgcaacct atgcaccccg caccgctccc tggtttgcaa ttcgcagctg
    24601 cttaacgaaa gtcaaattat cggtaccttt gagctgcagg gtccctcgcc tgacgaaaag
    24661 tccgcggctc cggggttgaa actcactccg gggctgtgga cgtcggctta ccttcgcaaa
    24721 tttgtacctg aggactacca cgcccacgag attaggttct acgaagacca atcccgcccg
    24781 cctaatgcgg agcttaccgc ctgcgtcatt acccagggcc acattcttgg ccaattgcaa
    24841 gccatcaaca aagcccgcca agagtttctg ctacgaaagg gacggggggt ttacttggac
    24901 ccccagtccg gcgaggagct caacccaatc cccccgccgc cgcagcccta tcagcagcag
    24961 ccgcgggccc ttgcttccca ggatggcacc caaaaagaag ctgcagctgc cgccgccacc
    25021 cacggacgag gaggaatact gggacagtca ggcagaggag gttttggacg aggaggagga
    25081 ggacatgatg gaagactggg agagcctaga cgaggaagct tccgaggtcg aagaggtgtc
    25141 agacgaaaca ccgtcaccct cggtcgcatt cccctcgccg gcgccccaga aatcggcaac
    25201 cggttccagc atggctacaa cctccgctcc tcaggcgccg ccggcactgc ccgttcgccg
    25261 acccaaccgt agatgggaca ccactggaac cagggccggt aagtccaagc agccgccgcc
    25321 gttagcccaa gagcaacaac agcgccaagg ctaccgctca tggcgcgggc acaagaacgc
    25381 catagttgct tgcttgcaag actgtggggg caacatctcc ttcgcccgcc gctttcttct
    25441 ctaccatcac ggcgtggcct tcccccgtaa catcctgcat tactaccgtc atctctacag
    25501 cccatactgc accggcggca gcggcagcaa cagcagcggc cacacagaag caaaggcgac
    25561 cggatagcaa gactctgaca aagcccaaga aatccacagc ggcggcagca gcaggaggag
    25621 gagcgctgcg tctggcgccc aacgaacccg tatcgacccg cgagcttaga aacaggattt
    25681 ttcccactct gtatgctata tttcaacaga gcaggggcca agaacaagag ctgaaaataa
    25741 aaaacaggtc tctgcgatcc ctcacccgca gctgcctgta tcacaaaagc gaagatcagc
    25801 ttcggcgcac gctggaagac gcggaggctc tcttcagtaa atactgcgcg ctgactctta
    25861 aggactagtt tcgcgccctt tctcaaattt aagcgcgaaa actacgtcat ctccagcggc
    25921 cacacccggc gccagcacct gttgtcagcg ccattatgag caaggaaatt cccacgccct
    25981 acatgtggag ttaccagcca caaatgggac ttgcggctgg agctgcccaa gactactcaa
    26041 cccgaataaa ctacatgagc gcgggacccc acatgatatc ccgggtcaac ggaatacgcg
    26101 cccaccgaaa ccgaattctc ctggaacagg cggctattac caccacacct cgtaataacc
    26161 ttaatccccg tagttggccc gctgccctgg tgtaccagga aagtcccgct cccaccactg
    26221 tggtacttcc cagagacgcc caggccgaag ttcagatgac taactcaggg gcgcagcttg
    26281 cgggcggctt tcgtcacagg gtgcggtcgc ccgggcaggg tataactcac ctgacaatca
    26341 gagggcgagg tattcagctc aacgacgagt cggtgagctc ctcgcttggt ctccgtccgg
    26401 acgggacatt tcagatcggc ggcgccggcc gctcttcatt cacgcctcgt caggcaatcc
    26461 taactctgca gacctcgtcc tctgagccgc gctctggagg cattggaact ctgcaattta
    26521 ttgaggagtt tgtgccatcg gtctacttta accccttctc gggacctccc ggccactatc
    26581 cggatcaatt tattcctaac tttgacgcgg taaaggactc ggcggacggc tacgactgaa
    26641 tgttataagt tcctgtccat ccgcacccac tatcttcatg ttgttgcaga tgaagcgcgc
    26701 aagaccgtct gaagatacct tcaaccccgt gtatccatat gacacggaaa ccggtcctcc
    26761 aactgtgcct tttcttactc ctccctttgt atcccccaat gggtttcaag agagtccccc
    26821 tggggtactc tctttgcgcc tatccgaacc tctagttacc tccaatggca tgcttgcgct
    26881 caaaatgggc aacggcctct ctctggacga ggccggcaac cttacctccc aaaatgtaac
    26941 cactgtgagc ccacctctca aaaaaaccaa gtcaaacata aacctggaaa tatctgcacc
    27001 cctcacagtt acctcagaag ccctaactgt ggctgccgcc gcacctctaa tggtcgcggg
    27061 caacacactc accatgcaat cacaggcccc gctaaccgtg cacgactcca aacttagcat
    27121 tgccacccaa ggacccctca cagtgtcaga aggaaagcta gccctgcaaa catcaggccc
    27181 cctcaccacc accgatagca gtacccttac tatcactgcc tcaccccctc taactactgc
    27241 cactggtagc ttgggcattg acttgaaaga gcccatttat acacaaaatg gaaaactagg
    27301 actaaagtac ggggctcctt tgcatgtaac agacgaccta aacactttga ccgtagcaac
    27361 tggtccaggt gtgactatta ataatacttc cttgcaaact aaagttactg gagccttggg
    27421 ttttgattca caaggcaata tgcaacttaa tgtagcagga ggactaagga ttgattctca
    27481 aaacagacgc cttatacttg atgttagtta tccgtttgat gctcaaaacc aactaaatct
    27541 aagactagga cagggccctc tttttataaa ctcagcccac aacttggata ttaactacaa
    27601 caaaggcctt tacttgttta cagcttcaaa caattccaaa aagcttgagg ttaacctaag
    27661 cactgccaag gggttgatgt ttgacgctac agccatagcc attaatgcag gagatgggct
    27721 tgaatttggt tcacctaatg caccaaacac aaatcccctc aaaacaaaaa ttggccatgg
    27781 cctagaattt gattcaaaca aggctatggt tcctaaacta ggaactggcc ttagttttga
    27841 cagcacaggt gccattacag taggaaacaa aaataatgat aagctaactt tgtggaccac
    27901 accagctcca tctcctaact gtagactaaa tgcagagaaa gatgctaaac tcactttggt
    27961 cttaacaaaa tgtggcagtc aaatacttgc tacagtttca gttttggctg ttaaaggcag
    28021 tttggctcca atatctggaa cagttcaaag tgctcatctt attataagat ttgacgaaaa
    28081 tggagtgcta ctaaacaatt ccttcctgga cccagaatat tggaacttta gaaatggaga
    28141 tcttactgaa ggcacagcct atacaaacgc tgttggattt atgcctaacc tatcagctta
    28201 tccaaaatct cacggtaaaa ctgccaaaag taacattgtc agtcaagttt acttaaacgg
    28261 agacaaaact aaacctgtaa cactaaccat tacactaaac ggtacacagg aaacaggaga
    28321 cacaactcca agtgcatact ctatgtcatt ttcatgggac tggtctggcc acaactacat
    28381 taatgaaata tttgccacat cctcttacac tttttcatac attgcccaag aataaagaat
    28441 cgtttgtgtt atgtttcaac gtgtttattt ttcaattgca gaaaatttca agtcattttt
    28501 cattcagtag tatagcccca ccaccacata gcttatacag atcaccgtac cttaatcaaa
    28561 ctcacagaac cctagtattc aacctgccac ctccctccca acacacagag tacacagtcc
    28621 tttctccccg gctggcctta aaaagcatca tatcatgggt aacagacata ttcttaggtg
    28681 ttatattcca cacggtttcc tgtcgagcca aacgctcatc agtgatatta ataaactccc
    28741 cgggcagctc acttaagttc atgtcgctgt ccagctgctg agccacaggc tgctgtccaa
    28801 cttgcggttg cttaacgggc ggcgaaggag aagtccacgc ctacatgggg gtagagtcat
    28861 aatcgtgcat caggataggg cggtggtgct gcagcagcgc gcgaataaac tgctgccgcc
    28921 gccgctccgt cctgcaggaa tacaacatgg cagtggtctc ctcagcgatg attcgcaccg
    28981 cccgcagcat aaggcgcctt gtcctccggg cacagcagcg caccctgatc tcacttaaat
    29041 cagcacagta actgcagcac agcaccacaa tattgttcaa aatcccacag tgcaaggcgc
    29101 tgtatccaaa gctcatggcg gggaccacag aacccacgtg gccatcatac cacaagcgca
    29161 ggtagattaa gtggcgaccc ctcataaaca cgctggacat aaacattacc tcttttggca
    29221 tgttgtaatt caccacctcc cggtaccata taaacctctg attaaacatg gcgccatcca
    29281 ccaccatcct aaaccagctg gccaaaacct gcccgccggc tatacactgc agggaaccgg
    29341 gactggaaca atgacagtgg agagcccagg actcgtaacc atggatcatc atgctcgtca
    29401 tgatatcaat gttggcacaa cacaggcaca cgtgcataca cttcctcagg attacaagct
    29461 cctcccgcgt tagaaccata tcccagggaa caacccattc ctgaatcagc gtaaatccca
    29521 cactgcaggg aagacctcgc acgtaactca cgttgtgcat tgtcaaagtg ttacattcgg
    29581 gcagcagcgg atgatcctcc agtatggtag cgcgggtttc tgtctcaaaa ggaggtagac
    29641 gatccctact gtacggagtg cgccgagaca accgagatcg tgttggtcgt agtgtcatgc
    29701 caaatggaac gccggacgta gtcatatttc ctgaagcaaa accaggtgcg ggcgtgacaa
    29761 acagatctgc gtctccggtc tcgccgctta gatcgctctg tgtagtagtt gtagtatatc
    29821 cactctctca aagcatccag gcgccccctg gcttcgggtt ctatgtaaac tccttcatgc
    29881 gccgctgccc tgataacatc caccaccgca gaataagcca cacccagcca acctacacat
    29941 tcgttctgcg agtcacacac gggaggagcg ggaagagctg gaagaaccat gttttttttt
    30001 ttattccaaa agattatcca aaacctcaaa atgaagatct attaagtgaa cgcgctcccc
    30061 tccggtggcg tggtcaaact ctacagccaa agaacagata atggcatttg taagatgttg
    30121 cacaatggct tccaaaaggc aaacggccct cacgtccaag tggacgtaaa ggctaaaccc
    30181 ttcagggtga atctcctcta taaacattcc agcaccttca accatgccca aataattctc
    30241 atctcgccac cttctcaata tatctctaag caaatcccga atattaagtc cggccattgt
    30301 aaaaatctgc tccagagcgc cctccacctt cagcctcaag cagcgaatca tgattgcaaa
    30361 aattcaggtt cctcacagac ctgtataaga ttcaaaagcg gaacattaac aaaaataccg
    30421 cgatcccgta ggtcccttcg cagggccagc tgaacataat cgtgcaggtc tgcacggacc
    30481 agcgcggcca cttccccgcc aggaaccatg acaaaagaac ccacactgat tatgacacgc
    30541 atactcggag ctatgctaac cagcgtagcc ccgatgtaag cttgttgcat gggcggcgat
    30601 ataaaatgca aggtgctgct caaaaaatca ggcaaagcct cgcgcaaaaa agaaagcaca
    30661 tcgtagtcat gctcatgcag ataaaggcag gtaagctccg gaaccaccac agaaaaagac
    30721 accatttttc tctcaaacat gtctgcgggt ttctgcataa acacaaaata aaataacaaa
    30781 aaaacattta aacattagaa gcctgtctta caacaggaaa aacaaccctt ataagcataa
    30841 gacggactac ggccatgccg gcgtgaccgt aaaaaaactg gtcaccgtga ttaaaaagca
    30901 ccaccgacag ctcctcggtc atgtccggag tcataatgta agactcggta aacacatcag
    30961 gttgattcac atcggtcagt gctaaaaagc gaccgaaata gcccggggga atacataccc
    31021 gcaggcgtag agacaacatt acagccccca taggaggtat aacaaaatta ataggagaga
    31081 aaaacacata aacacctgaa aaaccctcct gcctaggcaa aatagcaccc tcccgctcca
    31141 gaacaacata cagcgcttcc acagcggcag ccataacagt cagccttacc agtaaaaaag
    31201 aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca cagtgtaaaa
    31261 aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg gttaaagtcc
    31321 acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag ccaaaaaacc
    31381 cacaacttcc tcaaatcgtc acttccgttt tcccacgtta cgtcacttcc cattttaaga
    31441 aaactacaat tcccaacaca tacaagttac tccgccctaa aacctacgtc acccgccccg
    31501 ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt ggcttcaatc
    31561 caaaataagg tatattattg atgatgttac atcgttaatt aacgatttcg aacccggggt
    31621 accgaattcc tcgagtctag aggagcatgc gacgtcgcaa ttcgccctat agtgagtcgt
    31681 attacaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
    31741 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
    31801 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggaaa ttgtaagcgt
    31861 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
    31921 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
    31981 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
    32041 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
    32101 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
    32161 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
    32221 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
    32281 taatgcgccg ctacagggcg cgtcctgatg cggtattttc tccttacgca tctgtgcggt
    32341 atttcacacc gcatacaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
    32401 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
    32461 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    32521 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    32581 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    32641 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    32701 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    32761 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    32821 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    32881 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    32941 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    33001 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    33061 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    33121 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    33181 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    33241 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    33301 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    33361 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    33421 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    33481 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    33541 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    33601 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    33661 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    33721 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    33781 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    33841 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    33901 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    33961 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    34021 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    34081 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    34141 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    34201 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    34261 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
    34321 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    34381 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    34441 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
    34501 gctatgacca tgattacgcc aagctattta ggtgacacta tagaatactc aagctagtta
    34561 attaacgtta attaacatca tcaataatat accttatttt ggattgaagc caatatgata
    34621 atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg tgacgtagta
    34681 gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca
    34741 aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc gcgcggtttt
    34801 aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa
    34861 aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg cgtaatctct
    34921 agcat
//
