LOCUS       sequence-135095-        7111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Crepis alpina plastid omega-3 fatty acid desaturase (FAD7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Kappock
  TITLE     Additional fatty acid desaturase and acetylenase constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK276
FEATURES             Location/Qualifiers
     source          1..7111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(5..252)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     218..236
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(1621..1640)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1622..1640)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1672..2114)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(1678..1701)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    1997..2114
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     rep_origin      2133..2588
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2220..2239)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      2657..3537
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        4136..4356
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             4357..5160
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             join(5256..5324,5325..6116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLID*MEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6287..6875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6776..6795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7029..7046
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggccgcaaat taaagccttc gagcgtccca aaaccttctc aagcaaggtt ttcagtataa
       61 tgttacatgc gtacacgcgt ctgtacagaa aaaaaagaaa aatttgaaat ataaataacg
      121 ttcttaatac taacataact ataaaaaaat aaatagggac ctagacttca ggttgtctaa
      181 ctccttcctt ttcggttaga gcggatgtgg ggggagggcg tgaatgtaag cgtgacataa
      241 ctaattacat gatgcggccc tctagagtca ctatttagag gcaccagaga gctcagggtc
      301 ggtttgatag tatacaacat ccccttcatc actcacataa tgatcttttt tcatactact
      361 tgcaacaagt ccgagcaaat ggaatggaag tggccatgat ttctttggct ccctataata
      421 tttcccaagc actggcttcg cagcttccgt tgcctctatt aagttatagt gtgggatttg
      481 ggggaaaaga tgatgtatga catgggttcc tatgtcatga tggatgttat ttatccatcc
      541 atagtcacgg tcaagtgtgg ttagccctcc tcttaggtaa ctccattcct tgccacggta
      601 ccatggcagt ttgtcttcat ggccatggtg atgcaagtaa gttactaaat cgagccacat
      661 gacaaagccc acatagggga tcccatagag tttaagtact tggagagggc ccattacaaa
      721 ggacagaccc acaagaagag cagccatggc tgtccaacaa acggttgatg tgatcacatc
      781 tttcttctca ttaggcagaa ataaatcgct gtctgggtga taatgagagc ctttctttcc
      841 tggacttcgt agccacagat agaaaggata tgcaagcaaa gggaaaggca gagtgatccg
      901 cgtcaaccga gttacagtat ctaaacttct ataaagcttc tccgataatg gatgccaaga
      961 ttcatcattt tcaacatgtc catggttctg atgatgtgtt ctatggctaa ttctccatcc
     1021 atgataaggt acaagaattg aggaatgaag aagatggcca acaacactat tgagttttgc
     1081 attattagaa aagcttccat gcccacaatc atggccaaga acaaaaagag cccagaacat
     1141 tgttccttga gcagcccaat acagaggcca aaccaccata ttgttaaaat acgccgccac
     1201 cgcggctaac ccgaaaacca ccaccacatc tctcaccaca taactcatcg acttccatgg
     1261 attcttcacc cagcaatgtt ttgggatggc ggctctgata tcagccaatg tgaagggtgg
     1321 tggtgcaccg gggttgaact cactttctcc ggcgttaatc ccattcactt tttgttcatc
     1381 ctcttctata aattggacac ccaccggcgc actcaccttc agtgcccacc tcctatccct
     1441 tgaaaccgat aaatttggcc ggaaaatctt cgacggggct aatctgaccg aattcggaaa
     1501 gctggaactc gagattcttg ttctgggatt ggaatatagt ctgggaagtg gtcttaaacc
     1561 acattctgat aacacccagg tagccattgt ggatccgagc tcggtaccaa gcttaatatt
     1621 ccctatagtg agtcgtatta cagctgctag tagtccgatc cggggttttt tctccttgac
     1681 gttaaagtat agaggtatat taacaatttt ttgttgatac ttttattaca tttgaataag
     1741 aagtaataca aaccgaaaat gttgaaagta ttagttaaag tggttaatgc agtttttgca
     1801 tttatatatc tgttaataga tcaaaaatca tcgcttcgct gattaattac cccagaaata
     1861 aggctaaaaa actaatcgca ttatcatcct atggttgtta atttgattcg ttcatttgaa
     1921 ggtttgtggg gccaggttac tgccaatttt tcctcttcat aaccataaaa gctagtattg
     1981 tagaatcttt attgttcgga gcagtgcggc gcgaggcaca tctgcgtttc aggaacgcga
     2041 ccggtgagga cgaggacgca cggaggagag tcttccttcg gagggctgtc acccgctcgg
     2101 cggcttctaa tccgtactag tggatcatcc ccacgcgccc tgtagcgccc cattaagcgc
     2161 ggcgggtgtg gtggttacgc ccagcgtgac ccctacactt cccaccgccc tagcccccgc
     2221 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2281 aaatgggggc atccgtttac ccttccgatt tactgcttta cggcacctcg accccaaaaa
     2341 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagaccc tttttcgccc
     2401 tttgacgttg gagtccacgt tctttaatag tggactcttg ttggaaactg gaacaacact
     2461 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cgggctattc
     2521 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     2581 tacaatttaa atatttgctt atacaatctt cctgtttttg gggcttttct gattatcaac
     2641 cggggtggag cttcccattg cgaataccgc ttccacaaac attgctcaaa agtatctctt
     2701 tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt cgctccttga
     2761 acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt actctttaga
     2821 caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg aaaatgtaaa
     2881 catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa ttttctgacc
     2941 aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat ccacatcggt
     3001 atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct tcgctagtaa
     3061 tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata gacaccaaag
     3121 tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag actgcattat
     3181 agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa tagcgctctc
     3241 gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa aatagcgctc
     3301 tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga aaaattagcg
     3361 ctctcgtcgc gttgcatttt tgttttacaa aaatgaagca cagattcttc gttggtaaaa
     3421 tagcgctttc gcgttgcatt tctgttctgt aaaaatgcag ctcagattct ttgtttgaaa
     3481 aattagcgct ctcgcgttgc atttttgttc tacaaaatga agcacagatg cttcgttaac
     3541 aaagatatgc tattgaagtg caagatggaa acgcagaaaa tgaaccgggg atgcgacgtg
     3601 caagattacc tatgcaatag atgcaatagt ttctccagga accgaaatac atacattgtc
     3661 ttccgtaaag cgctagacta tatattatta tacaggttca aatatactat ctgtttcagg
     3721 gaaaactccc aggttcggat gttcaaaatt caatgatggg taacaagtac gatcgtaaat
     3781 ctgtaaaaca gtttgtcgga tattaggctg tatctcctca aagcgtattc gaatatcatt
     3841 gagaagctgc agcgtcacat cggataataa tgatggcagc cattgtagaa gtgccttttg
     3901 catttctagt ctctttctcg gtctagctag ttttactaca tcgcgaagat agaatcttag
     3961 atcacactgc ctttgctgag ctggatcaat agagtaacaa aagagtggta aggcctcgtt
     4021 aaaggacaag gacctgagcg gaagtgtatc gtacagtaga cggagtatct agtatagtct
     4081 atagtccgtg gaattaattc tcatctttga cagcttatca tcgataagct agcttttcaa
     4141 ttcaattcat catttttttt ttattctttt ttttgatttc ggtttctttg aaattttttt
     4201 gattcggtaa tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta
     4261 tatatacgca tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg
     4321 cacagaacaa aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa
     4381 cgtgctgcta ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag
     4441 caaacaaact tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt
     4501 gaagcattag gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt
     4561 tccatggagg gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc
     4621 ttcgaagaca gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt
     4681 gtatacagaa tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt
     4741 attgttagcg gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg
     4801 atgttagcag aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact
     4861 gttgacattg cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg
     4921 ggtggaagag atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac
     4981 aagggagacg cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct
     5041 gacattatta ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt
     5101 gaacgttaca gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa
     5161 aaaactgtat tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa
     5221 ttatatcagt tattacccat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     5281 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     5341 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     5401 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     5461 cttttaaagt tctgctatgt gatacactat tatcccgtat tgacgccggg caagagcaac
     5521 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5581 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5641 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5701 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5761 aagccatacc aaacgacgag agtgacacca cgatgcctgt agcaatgcca acaacgttgc
     5821 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgaa
     5881 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5941 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6001 cagatggtaa gcgctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6061 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     6121 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     6181 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     6241 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6301 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6361 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6421 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6481 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6541 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6601 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6661 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6721 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6781 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6841 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6901 ggttcctggg cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6961 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7021 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7081 tccccgcgcg ttggccgatt cattaatgca g
//
