LOCUS       sequence-135098-       14558 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid contains left and right homology arms for human GDF8 
            flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14558)
  AUTHORS   Xu L, Zhao P, Mariano A, Han R
  TITLE     Targeted Myostatin Gene Editing in Multiple Mammalian Species 
            Directed by a Single Pair of TALE Nucleases.
  JOURNAL   Mol Ther Nucleic Acids. 2013 Jul 30;2:e112. doi: 
            10.1038/mtna.2013.39.
  PUBMED    23900226
REFERENCE   2  (bases 1 to 14558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Nucleic Acids."; date: "2013-07-30"; volume: "2:e112. doi"; pages: "
            10.1038/mtna.2013.39"
COMMENT     Sequence Label: hGDF8 donor CFP-hDF
FEATURES             Location/Qualifiers
     source          1..14558
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1325..1628
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1629..1832
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1783..1803
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    2080..2105
                     /label=SA
                     /note="splice acceptor site"
     CDS             2123..2176
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2186..2782
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2186..2205)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2682..2702
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2801..2854
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2867..2876
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2873..2875,2876..2878,2879..3589)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2918..2939)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3179..3198)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3526..3547
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4292..4303
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10028..10149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10065..10084)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10119..10138
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10930..10949)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10931..10949)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10956..10973)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10956..10972)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10965..10987)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             11110..11412
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     11291..11310
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             11761..12555
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11761..12555
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11815..11834)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12425..12444
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(12805..13596,13597..13665))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13428..13447
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13789..14377
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14278..14297
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14531..14548
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca
      241 gaattaaccc tcactaaagg gactagtgcc tggccctaaa gacaatcagg aaagagaaac
      301 aggcaccttg acagagaagg catggcaaga aaaattttgt gttacctgtt atcttgcttt
      361 aatacaatgc tttatatact ttaaacaaga ctccaaacag ttttaaaata ttatttccct
      421 tattaagtaa tcaggttaca atgcagcaaa gaatttccat ttaagactct gctatcaaat
      481 aggtctggag cagatttacc ttttatagac aattttactt tcgaactttt cagatagcct
      541 gaatataaca tcaaaatttt tgtgttaatt atctgcttag ttttgctcct ttaaaaggtt
      601 attccaaagc caaaatgtaa cagatgtact gtgttttcta ctaattcctg aggctcagta
      661 agttgctcag tgtgtcttgt ccccaggtaa ttcaggcctg ggggaagggt tccttcttcc
      721 agactgattg gtacagctgc tcagtaagtg taactactca gattcccaaa gaattctaag
      781 tggttgttcc tccacagtgt ctcttgttct ctctaatcat catcatttta aaatttcatt
      841 cagcgttcat tacttcatag aaccttcctt tgtccatagt tctctggaag gggaatagat
      901 tcctgatgaa cagctgaaaa tacatacgtc tgaaaaattc tgaaaagcta gggtaattat
      961 tttctttgac atttttttga gttataaagt tccacacggt atttcatttt aaaagactgc
     1021 ataggcatac attatcttaa tttaaaaaaa tgctcaacta ttaatatgga ggggttttgt
     1081 taatgggaaa taatttcagc aacttttctt ttcttattca tttatagctg attttctaat
     1141 gcaagtggat ggaaaaccca aatgttgctt ctttaaattt agctctaaaa tacaatacaa
     1201 taaagtagta aaggcccaac tatggatata tttgagaccc gtcgagactc ctacaatcta
     1261 gaggtaccta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     1321 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1381 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     1441 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1501 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1561 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1621 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     1681 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     1741 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     1801 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
     1861 tcttccagcc ccctgtcatg gcatcttcca ggggtccgag agctcagcta gtcttcttcc
     1921 tccaacccgg gcccctatgt ccacttcagg acagcatgtt tgctgcctcc agggatcctg
     1981 tgtccccgag ctgggaccac cttatattcc cagggccggt taatgtggct ctggttctgg
     2041 gtacttttat ctgtcccctc caccccacag tggggccttc tgacctcttc tcttcctccc
     2101 acagggcggc gggtccggag gagagggcag aggaagtctt ctaacatgcg gtgacgtgga
     2161 ggagaatcct ggcccaatgt cgagtatgac cgagtacaag cccacggtgc gcctcgccac
     2221 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     2281 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     2341 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     2401 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     2461 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     2521 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
     2581 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     2641 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     2701 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     2761 catgacccgc aagcccggtg ccggcggcgg gtccggagga gagggcagag gaagtcttct
     2821 aacatgcggt gacgtggagg agaatcctgg cccaatggga ccggtcgcca ccatggtgag
     2881 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     2941 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     3001 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     3061 caccctgacc tggggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     3121 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     3181 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     3241 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     3301 gtacaactac atcagccaca acgtctatat caccgccgac aagcagaaga acggcatcaa
     3361 ggccaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     3421 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     3481 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     3541 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagc gaattctgca
     3601 gtcgacggta ccgatgctga gggtcttcat cctctatgcc gagaacgtcc acacacccga
     3661 caccgacatc agcgatgcct actgctccgc ggtgtttgca ggggtgaaga agagaaccaa
     3721 agtcatcaag aacagcgtga accctgtatg gaatgaggga tttgaatggg acctcaaggg
     3781 catccccctg gaccagggct ctgagcttca tgtggtggtc aaagaccatg agacgatggg
     3841 gaggaacagg ttcctggggg aagccaaggt cccactccga gaggtcctcg ccacccctag
     3901 tctgtccgcc agcttcaatg cccccctgct ggacaccaag aagcagccca caggggcctc
     3961 gctggtcctg caggtgtcct acacaccgct gcctggagct gtgcctctgt tcccgccccc
     4021 tactcctctg gagccctccc cgactctgcc tgacctggat gtagtggcag acacaggagg
     4081 agaggaagac acagaggacc agggactcac tggagatgag gcggagccat tcctggatca
     4141 aagcggaggc ccgggggctc ccaccacccc aaggaaacta ccttcacgtc ctccgcccca
     4201 ctaccccggg atcaaaagaa agcgaagtgc gcctacatct agaaagctgc tgtcagacaa
     4261 accgcaggat ttccagatca gggtccaggt gatcgagggg cgccagctgc cgggggtgaa
     4321 catcaagcct gtggtcaagg ttaccgctgc agggcagacc aagcggacgc ggatccacaa
     4381 gggaaacagc ccactcttca atgagactct tttcttcaac ttgtttgact ctcctgggga
     4441 gctgtttgat gagcccatct ttatcacggt ggtagactct cgttctctca ggacagatgc
     4501 tctcctcggg gagttccgga tggacgtggg caccatttac agagagcccc ggcatgccta
     4561 tctcaggaag tggctgctgc tctcagaccc tgatgacttc tctgctgggg ccagaggcta
     4621 cctgaaaaca agcctttgtg tgctggggcc tggggacgaa gcgcctctgg agagaaaaga
     4681 cccctctgaa gacaaggagg acattgaaag caacctgctc cggcccacag gcgtagccct
     4741 gcgaggagcc cacttctgcc tgaaggtctt ccgggccgag gacttgccgc agatggacga
     4801 tgccgtgatg gacaacgtga aacagatctt tggcttcgag agtaacaaga agaacttggt
     4861 ggaccccttt gtggaggtca gctttgcggg gaaaatgctg tgcagcaaga tcttggagaa
     4921 gacggccaac cctcagtgga accagaacat cacactgcct gccatgtttc cctccatgtg
     4981 cgaaaaaatg aggattcgta tcatagactg ggaccgcctg actcacaatg acatcgtggc
     5041 taccacctac ctgagtatgt cgaaaatctc tgcccctgga ggagaaatag aagaggagcc
     5101 tgcaggtgct gtcaagcctt cgaaagcctc agacttggat gactacctgg gcttcctccc
     5161 cacttttggg ccctgctaca tcaacctcta tggcagtccc agagagttca caggcttccc
     5221 agacccctac acagagctca acacaggcaa gggggaaggt gtggcttatc gtggccggct
     5281 tctgctctcc ctggagacca agctggtgga gcacagtgaa cagaaggtgg aggaccttcc
     5341 tgcggatgac atcctccggg tggagaagta ccttaggagg cgcaagtact ccctgtttgc
     5401 ggccttctac tcagccacca tgctgcagga tgtggatgat gccatccagt ttgaggtcag
     5461 catcgggaac tacgggaaca agttcgacat gacctgcctg ccgctggcct ccaccactca
     5521 gtacagccgt gcagtctttg acgggtgcca ctactactac ctaccctggg gtaacgtgaa
     5581 acctgtggtg gtgctgtcat cctactggga ggacatcagc catagaatcg agactcagaa
     5641 ccagctgctt gggattgctg accggctgga agctggcctg gagcaggtcc acctggccct
     5701 gaaggcgcag tgctccacgg aggacgtgga ctcgctggtg gctcagctga cggatgagct
     5761 catcgcaggc tgcagccagc ctctgggtga catccatgag acaccctctg ccacccacct
     5821 ggaccagtac ctgtaccagc tgcgcaccca tcacctgagc caaatcactg aggctgccct
     5881 ggccctgaag ctcggccaca gtgagctccc tgcagctctg gagcaggcgg aggactggct
     5941 cctgcgtctg cgtgccctgg cagaggagcc ccagaacagc ctgccggaca tcgtcatctg
     6001 gatgctgcag ggagacaagc gtgtggcata ccagcgggtg cccgcccacc aagtcctctt
     6061 ctcccggcgg ggtgccaact actgtggcaa gaattgtggg aagctacaga caatctttct
     6121 gaaatatccg atggagaagg tgcctggcgc ccggatgcca gtgcagatac gggtcaagct
     6181 gtggtttggg ctctctgtgg atgagaagga gttcaaccag tttgctgagg ggaagctgtc
     6241 tgtctttgct gaaacctatg agaacgagac taagttggcc cttgttggga actggggcac
     6301 aacgggcctc acctacccca agttttctga cgtcacgggc aagatcaagc tacccaagga
     6361 cagcttccgc ccctcggccg gctggacctg ggctggagat tggttcgtgt gtccggagaa
     6421 gactctgctc catgacatgg acgccggtca cctgagcttc gtggaagagg tgtttgagaa
     6481 ccagacccgg cttcccggag gccagtggat ctacatgagt gacaactaca ccgatgtgaa
     6541 cggggagaag gtgcttccca aggatgacat tgagtgccca ctgggctgga agtgggaaga
     6601 tgaggaatgg tccacagacc tcaaccgggc tgtcgatgag caaggctggg agtatagcat
     6661 caccatcccc ccggagcgga agccgaagca ctgggtccct gctgagaaga tgtactacac
     6721 acaccgacgg cggcgctggg tgcgcctgcg caggagggat ctcagccaaa tggaagcact
     6781 gaaaaggcac aggcaggcgg aggcggaggg cgagggctgg gagtacgcct ctctttttgg
     6841 ctggaagttc cacctcgagt accgcaagac agatgccttc cgccgccgcc gctggcgccg
     6901 tcgcatggag ccactggaga agacggggcc tgcagctgtg tttgcccttg agggggccct
     6961 gggcggcgtg atggatgaca agagtgaaga ttccatgtcc gtctccacct tgagcttcgg
     7021 tgtgaacaga cccacgattt cctgcatatt cgactatggg aaccgctacc atctacgctg
     7081 ctacatgtac caggcccggg acctggctgc gatggacaag gactcttttt ctgatcccta
     7141 tgccatcgtc tccttcctgc accagagcca gaagacggtg gtggtgaaga acacccttaa
     7201 ccccacctgg gaccagacgc tcatcttcta cgagatcgag atctttggcg agccggccac
     7261 agttgctgag caaccgccca gcattgtggt ggagctgtac gaccatgaca cttatggtgc
     7321 agacgagttt atgggtcgct gcatctgtca accgagtctg gaacggatgc cacggctggc
     7381 ctggttccca ctgacgaggg gcagccagcc gtcgggggag ctgctggcct cttttgagct
     7441 catccagaga gagaagccgg ccatccacca tattcctggt tttgaggtgc aggagacatc
     7501 aaggatcctg gatgagtctg aggacacaga cctgccctac ccaccacccc agagggaggc
     7561 caacatctac atggttcctc agaacatcaa gccagcgctc cagcgtaccg ccatcgagat
     7621 cctggcatgg ggcctgcgga acatgaagag ttaccagctg gccaacatct cctcccccag
     7681 cctcgtggta gagtgtgggg gccagacggt gcagtcctgt gtcatcagga acctccggaa
     7741 gaaccccaac tttgacatct gcaccctctt catggaagtg atgctgccca gggaggagct
     7801 ctactgcccc cccatcaccg tcaaggtcat cgataaccgc cagtttggcc gccggcctgt
     7861 ggtgggccag tgtaccatcc gctccctgga gagcttcctg tgtgacccct actcggcgga
     7921 gagtccatcc ccacagggtg gcccagacga tgtgagccta ctcagtcctg gggaagacgt
     7981 gctcatcgac attgatgaca aggagcccct catccccatc caggaggaag agttcatcga
     8041 ttggtggagc aaattctttg cctccatagg ggagagggaa aagtgcggct cctacctgga
     8101 gaaggatttt gacaccctga aggtctatga cacacagctg gagaatgtgg aggcctttga
     8161 gggcctgtct gacttttgta acaccttcaa gctgtaccgg ggcaagacgc aggaggagac
     8221 agaagatcca tctgtgattg gtgaatttaa gggcctcttc aaaatttatc ccctcccaga
     8281 agacccagcc atccccatgc ccccaagaca gttccaccag ctggccgccc agggacccca
     8341 ggagtgcttg gtccgtatct acattgtccg agcatttggc ctgcagccca aggaccccaa
     8401 tggaaagtgt gatccttaca tcaagatctc catagggaag aaatcagtga gtgaccagga
     8461 taactacatc ccctgcacgc tggagcccgt atttggaaag atgttcgagc tgacctgcac
     8521 tctgcctctg gagaaggacc taaagatcac tctctatgac tatgacctcc tctccaagga
     8581 cgaaaagatc ggtgagacgg tcgtcgacct ggagaacagg ctgctgtcca agtttggggc
     8641 tcgctgtgga ctcccacaga cctactgtgt ctctggaccg aaccagtggc gggaccagct
     8701 ccgcccctcc cagctcctcc acctcttctg ccagcagcat agagtcaagg cacctgtgta
     8761 ccggacagac cgtgtaatgt ttcaggataa agaatattcc attgaagaga tagaggctgg
     8821 caggatccca aacccacacc tgggcccagt ggaggagcgt ctggctctgc atgtgcttca
     8881 gcagcagggc ctggtcccgg agcacgtgga gtcacggccc ctctacagcc ccctgcagcc
     8941 agacatcgag caggggaagc tgcagatgtg ggtcgaccta tttccgaagg ccctggggcg
     9001 gcctggacct cccttcaaca tcaccccacg gagagccaga aggtttttcc tgcgttgtat
     9061 tatctggaat accagagatg tgatcctgga tgacctgagc ctcacggggg agaagatgag
     9121 cgacatttat gtgaaaggtt ggatgattgg ctttgaagaa cacaagcaaa agacagacgt
     9181 gcattatcgt tccctgggag gtgaaggcaa cttcaactgg aggttcattt tccccttcga
     9241 ctacctgcca gctgagcaag tctgtaccat tgccaagaag gatgccttct ggaggctgga
     9301 caagactgag agcaaaatcc cagcacgagt ggtgttccag atctgggaca atgacaagtt
     9361 ctcctttgat gattttctgg gctccctgca gctcgatctc aaccgcatgc ccaagccagc
     9421 caagacagcc aagaagtgct ccttggacca gctggatgat gctttccacc ccgaatggtt
     9481 tgtgtccctt tttgagcaga aaacagtgaa gggctggtgg ccctgtgtag cagaagaggg
     9541 tgagaagaaa atactggcgg gcaagctgga aatgaccttg gagattgtag cagagagtga
     9601 gcatgaggag cggcctgctg gccagggccg ggatgagccc aacatgaacc ctaagcttga
     9661 ggacccaagg cgccccgaca cctccttcct gtggtttacc tccccataca agaccatgaa
     9721 gttcatcctg tggcggcgtt tccggtgggc catcatcctc ttcatcatcc tcttcatcct
     9781 gctgctgttc ctggccatct tcatctacgc cttcccgaac tatgctgcca tgaagctggt
     9841 gaagcccttc agctgaggac tctcctgccc tgtagaaggg gccggtaccg cgggcccggg
     9901 atccaccgga tctagataac tgatcataat cagccatacc acatttgtag aggttttact
     9961 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    10021 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10081 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10141 tgtatcttag atatcctctg aaacttgaca tgaacccagg cactggtatt tggcagagca
    10201 ttgatgtgaa gacagtgttg caaaattggc tcaaacaacc tgaatccaac ttaggcattg
    10261 aaataaaagc tttagatgag aatggtcatg atcttgctgt aaccttccca ggaccaggag
    10321 aagatgggct ggtaagtgat aactgaaaat aacattataa taaccttatg tttttattca
    10381 taatatgaac aaacaatagt ggaaaatagc tacaaattcc ctaagctcat aagctagaca
    10441 aaggtatctt accccaacgg tagccctgta cccaataaaa gtaggtgtcc aatttcatat
    10501 cctatgaaac actcccttga tactcttact ttgcatcaag attttagaaa acaattatac
    10561 catacttctt aacttcttaa gaagtccttt tgaattggga atgaaatata aagtgctttt
    10621 cattaatatg atacatgact gtatgtatta aaatattaac tctatatagt ggattttacc
    10681 acataaacca acaaatccaa acattatttt tttctcccag aagggtgcca aatgtgttaa
    10741 agatttttgg cttagcataa aacagataaa ccttttaaaa ttataattaa atgttttatt
    10801 cagagaaatt aagagtgata tttataggct tatactttat taaatatatt taaggtttct
    10861 caagataaat atgttcatta tttgtaggat gttgatgcac tgatgtgggc ggccgctaaa
    10921 ttcaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
    10981 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
    11041 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagccta
    11101 tacgtacggc agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg
    11161 gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc cctggccagt
    11221 gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat
    11281 gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa
    11341 gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
    11401 tggggaatat aaatgtcagg catgagatta tcaaaaagga tcttcaccta gatccttttc
    11461 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
    11521 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
    11581 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
    11641 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
    11701 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc
    11761 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
    11821 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
    11881 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
    11941 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
    12001 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
    12061 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
    12121 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
    12181 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    12241 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
    12301 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    12361 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    12421 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    12481 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    12541 gacgagttct tctgaattat taacgcttac aatttcctga tgcggtattt tctccttacg
    12601 catctgtgcg gtatttcaca ccgcatcagg tggcactttt cggggaaatg tgcgcggaac
    12661 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gattatcaaa
    12721 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
    12781 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
    12841 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
    12901 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
    12961 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
    13021 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
    13081 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
    13141 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
    13201 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
    13261 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
    13321 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
    13381 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
    13441 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
    13501 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
    13561 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
    13621 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
    13681 atattattga agcatttatc agggttattg tctcatgacc aaaatccctt aacgtgagtt
    13741 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    13801 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    13861 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    13921 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    13981 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    14041 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    14101 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    14161 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    14221 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    14281 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    14341 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    14401 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    14461 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    14521 gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
