LOCUS       sequence-135114-        1101 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for 
            sgRNA insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Schwartz CM, Hussain MS, Blenner M, Wheeldon I
  TITLE     Synthetic RNA polymerase III promoters facilitate high efficiency 
            CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2015 Dec 29.
  PUBMED    26714206
REFERENCE   2  (bases 1 to 1101)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 29."
COMMENT     Sequence Label: pCRISPRyl
FEATURES             Location/Qualifiers
     source          1..1101
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        500..575
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     625..643
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 aaataaacaa ataggggttc cgcacacatt tccccgaaaa gtgccacctg acgtccccag
       61 ttgcaaaagt tgacacaact ctagatctgc ttccaaatat agaatcataa caagggttag
      121 ggtgtgatta tataatattg gtcttaattg atgtgctagg gctttaaaag ttggttaaaa
      181 taacgctcta atgccttttt aatatattgt ctttttcaaa atctcaaatc ggacacttct
      241 tcgtgtatga gactccattt tttggctccg tcacgtgata tgtattatca gctatagtgg
      301 tgtaaacaaa gttttttact agctgtaatg gcattttgtc ggagtggtaa atcgccttct
      361 tgttgtgcgt tcgagttctg gactctgcac tgggctactt tgaaaaatac ctctaatgcg
      421 ccgatggttt agtggtaaaa tccatcgttg ccatcgatgg gcccccggtt cgattccggg
      481 tcggcgcagg ttgacctagg ttttagagct agaaatagca agttaaaata aggctagtcc
      541 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttacgtcta agaaaccatt
      601 attatcatga cattaaccta taaaaatagg cgtatcacga ggcccagatc ctctagagtc
      661 gaagcggccg ctatgtctga taaaaggatg taacataggc aagctgctcg tgagtgttga
      721 gtacgaacct tagatccaaa tcacccgcac ccacggatat acttgcttga atatacagta
      781 gtatgcggcc gcttcgacac catatcatat aaaactaaca atgcattgaa ttaatacgaa
      841 gactacccgt tgctatctcc acaccgttat ctccacggtc caaaggctgc tcaatgtgct
      901 gcatacgtaa cgtggggtgc aaccttgagc acatagtact ttccgaaaac cggcgataat
      961 taagtgtgca ctccaacttt tcacactgag cataaaatgt ggagaagaaa tcacactaaa
     1021 aagtcaggta gactggaaaa tgcgccatga aataatatct cttgctacag taatgcccag
     1081 catcgagggg tattgtgtca c
//
