LOCUS       sequence-135115-        1118 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPR/Cas9 vector for Yarrowia lipolytica, with AvrII site for 
            sgRNA insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Schwartz CM, Hussain MS, Blenner M, Wheeldon I
  TITLE     Synthetic RNA polymerase III promoters facilitate high efficiency 
            CRISPR-Cas9 mediated genome editing in Yarrowia lipolytica.
  JOURNAL   ACS Synth Biol. 2015 Dec 29.
  PUBMED    26714206
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 29."
COMMENT     Sequence Label: pCRISPRyl
FEATURES             Location/Qualifiers
     source          1..1118
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(4..251)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     217..235
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     CDS             complement(262..282)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cttgcaaatt aaagccttcg agcgtcccaa aaccttctca agcaaggttt tcagtataat
       61 gttacatgcg tacacgcgtc tgtacagaaa aaaaagaaaa atttgaaata taaataacgt
      121 tcttaatact aacataacta taaaaaaata aatagggacc tagacttcag gttgtctaac
      181 tccttccttt tcggttagag cggatgtgga gggagggcgt gaatgtaagc gtgacataac
      241 taattacatg aggctagctt acacctttcg cttcttcttg gggtcggctc gagagtcgcc
      301 gcccagctga gacaggtcga ttcgggtctc gtacaggccg gtgatagact ggtggatcag
      361 ggtggcgtcc agcacctcct tggtagaggt gtatcgcttt cggtcgatgg tggtgtcgaa
      421 gtacttgaag gcagcggggg ctcccaggtt ggtcagggtg aacaggtgaa tgatgttctc
      481 agcctgctct cgaatgggct tgtctcggtg cttgttgtaa gcagacagca ccttatccag
      541 gttggcgtca gccaggatca ctcgcttaga gaactcagag atctgctcga tgatctcgtc
      601 caggtagtgc ttatgctgct ccacgaagag ctgcttctgc tcgttatcct cgggagagcc
      661 cttcagcttc tcgtaatggg aggcgaggta caggaagttg acgtacttgg agggcagggc
      721 cagctcgttt cccttctgca gctcgccggc agaggccagc attcgctttc gtccgttctc
      781 cagctcgaac agagagtact tggggagctt gataatcaga tctttcttca cctccttgta
      841 tcccttggcc tccaggaagt caatggggtt cttctcgaaa gaagatcgct ccatgatggt
      901 aatgcccagc agctccttca cagacttcag cttcttagac ttgcccttct ccaccttggc
      961 caccaccagc acagagtaag ccacggtggg agagtcgaaa cctccgtact tcttggggtc
     1021 ccagtccttc tttcgggcga tcagcttgtc agagtttcgc ttgggcagga tggactcctt
     1081 agagaaacct ccggtctgca cctcggtctt cttcacga
//
