LOCUS       sequence-135125-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Split GFP assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Kodaka M, Yang Z, Nakagawa K, Maruyama J, Xu X, Sarkar A, Ichimura 
            A, Nasu Y, Ozawa T, Iwasa H, Ishigami-Yuasa M, Ito S, Kagechika H, 
            Hata Y
  TITLE     A new cell-based assay to evaluate myogenesis in mouse myoblast 
            C2C12 cells.
  JOURNAL   Exp Cell Res. 2015 Aug 15;336(2):171-81. doi: 
            10.1016/j.yexcr.2015.06.015. Epub 2015 Jun 24.
  PUBMED    26116467
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.yexcr.2015.06.015"; journalName: "Exp Cell Res"; date: 
            "2015-08-15- 15"; volume: "336"; issue: "2"; pages: "171-81"
COMMENT     Sequence Label: pQCXIP-BSR-GFP11
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(164..187)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(188..235)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     CDS             complement(248..643)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPIFEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 agggccaggt ttccgggccc tcacattgcc aaaagacggc aatatggtgg aaaataacat
       61 atagacaaac gcacaccggc cttattccaa gcggcttcgg ccagtaacgt tagggggggg
      121 ggagggagag gggggggggg ggggggggcg gaattcgata tcacttgtcg tcgtcgtcct
      181 tgtagtcagt gatcccggcg gcgttcacgt actcgtgcag gaccatgtga tcgcgctgca
      241 gggatccgcc ctcccacaca taaccagagg gcagcaattc acgaatccca actgccgtcg
      301 gctgtccatc actgtccttc actatggctt tgatcccagg atgcaaatcg agaagcacct
      361 gtcggcaccg tccgcagggg ctcaagatgc ccctgttctc atttccgatc gcgacgatac
      421 aagtcaggtt gccagctgcc gcagcagcag cagtgcccag caccacgagt tctgcacaag
      481 gtcccccagt aaaatgatat acattgacac cagtgaagat gcggccgtcg ctagagagag
      541 ctgcgctggc gacgctgtag tcttcaaaga tggggatgct gttgattgta gccgttgctc
      601 tttcaatgag ggtggattct tcttgagaca aaggcttggc catggtttag ttcctcacct
      661 tgcggccgcg gatcaattcc tgcaggtccg ttccaatgca ccgttcccgg ccgcggaggc
      721 tggatcggtc ccggtgtctt ctatggaggt caaaacagcg
//
