LOCUS       sequence-135137-        9380 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-CmCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..9380
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1770..1814
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1867..1914
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1948..1966
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1967..1991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2006..2028
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3302..3346
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3399..3446
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3480..3498
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3499..3523
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3538..3560
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4315..4359
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4412..4459
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4503..4958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4800..4821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5073..5864,5865..5933))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5696..5715
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5934..6026)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6107..6695
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6596..6615
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6849..6866
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6881..7023
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6949..6968)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7109..7131
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7125..7316)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7888..8970)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8932..8951
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8971..9048)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9254..9273
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9364..9380,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcgcca caagaacaag cagtttctca ggtgatggag aaacagagca
      121 acaacaacaa cagtgacgtc attttccgat caaagttacc ggatatttac atcccgaacc
      181 acctatctct ccacgactac atcttccaaa acatctccga attcgccact aagccttgcc
      241 taatcaacgg accaaccggc cacgtgtaca cttactccga cgtccacgtc atctcccgcc
      301 aaatcgccgc caattttcac aaactcggcg ttaaccaaaa cgacgtcgtc atgctcctcc
      361 tcccaaactg tcccgaattc gtcctctctt tcctcgccgc ctccttccgc ggcgcaaccg
      421 ccaccgccgc aaaccctttc ttcactccgg cggagatagc taaacaagcc aaagcctcca
      481 acaccaaact cataatcacc gaagctcgtt acgtcgacaa aatcaaacca cttcaaaacg
      541 acgacggagt agtcatcgtc tgcatcgacg acaacgaatc cgtgccaatc cctgaaggct
      601 gcctccgctt caccgagttg actcagtcga caaccgaggc atcagaagtc atcgactcgg
      661 tggagatttc accggacgac gtggtggcac taccttactc ctctggcacg acgggattac
      721 caaaaggagt gatgctgact cacaagggac tagtcacgag cgttgctcag caagtcgacg
      781 gcgagaaccc gaatctttat ttccacagcg atgacgtcat actctgtgtt ttgcccatgt
      841 ttcatatcta cgctttgaac tcgatcatgt tgtgtggtct tagagttggt gcggcgattc
      901 tgataatgcc gaagtttgag atcaatctgc tattggagct gatccagagg tgtaaagtga
      961 cggtggctcc gatggttccg ccgattgtgt tggccattgc gaagtcttcg gagacggaga
     1021 agtatgattt gagctcgata agagtggtga aatctggtgc tgctcctctt ggtaaagaac
     1081 ttgaagatgc cgttaatgcc aagtttccta atgccaaact cggtcaggga tacggaatga
     1141 cggaagcagg tccagtgctc gcaatgtcgt taggttttgc aaaggaacct tttccggtta
     1201 agtcaggagc ttgtggtact gttgtaagaa atgctgagat gaaaatagtt gatccagaca
     1261 ccggagattc tctttcgagg aatcaacccg gtgagatttg tattcgtggt caccagatca
     1321 tgaaaggtta cctcaacaat ccggcagcta cagcagagac cattgataaa gacggttggc
     1381 ttcatactgg agatattgga ttgatcgatg acgatgacga gcttttcatc gttgatcgat
     1441 tgaaagaact tatcaagtat aaaggttttc aggtagctcc ggctgagcta gaggctttgc
     1501 tcatcggtca tcctgacatt actgatgttg ctgttgtcgc aatgaaagaa gaagcagctg
     1561 gtgaagttcc tgttgcattt gtggtgaaat cgaaggattc ggagttatca gaagatgatg
     1621 tgaagcaatt cgtgtcgaaa caggttgtgt tttacaagag aatcaacaaa gtgttcttca
     1681 ctgaatccat tcctaaagct ccatcaggga agatattgag gaaagatctg agggcaaaac
     1741 tagcaaatgg attgtgactc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc
     1801 agcacatgga ctcgtctact agtcgcagct taattaacct aaactgctgc caccgctgag
     1861 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctagga
     1921 tcgagatcga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
     1981 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc
     2041 tacggtccaa gaaatccgca acgctcaacg cgcagatggt ccggcgacgg tcctggcaat
     2101 cggcacggca accccggctc atagcgtgaa ccaggcagat tatccggact attactttcg
     2161 tattaccaaa tctgaacaca tgacggaact gaaagaaaaa ttcaaacgta tgtgcgataa
     2221 aagtatgatt aaaaaacgct acatgtacct gaccgaagaa atcctgaaag aaaacccgaa
     2281 tatgtgtgcc tacatggcac cgagcctgga tgcgcgccag gacattgtgg ttgtcgaagt
     2341 tccgaaactg ggtaaagaag cggccaccaa agccatcaaa gaatggggcc aaccgaaatc
     2401 aaaaattacg cacctgatct tttgcaccac gtcgggtgtg gatatgccgg gtgcagacta
     2461 tcagctgacc aaactgctgg gtctgcgtcc gagcgttaaa cgctttatga tgtaccagca
     2521 aggctgcttc gcaggcggta cggtcctgcg tctggctaaa gatctggcgg aaaacaataa
     2581 aggtgctcgc gttctggtgg tttgtagtga aattaccgct gtcacgtttc gtggtccggc
     2641 ggatacccat ctggactccc tggttggcca ggccctgttc ggcgatggtg cagctgcggt
     2701 tatcgtcggc gcagatccgg acacgagtgt ggaacgtccg ctgtatcagc tggtttcaac
     2761 ctcgcaaacg attctgccgg attccgacgg tgcgatcgat ggccatctgc gcgaagtggg
     2821 tctgaccttt cacctgctga aagacgttcc gggcctgatt tcaaaaaaca tcgaaaaaag
     2881 cctgtctgaa gcctttgcac cggttggtat ttcggattgg agctctattt tctggatcgc
     2941 acatccgggc ggtccggcaa tcctggacca ggtggaaagc aaactgggtc tgaaagaaga
     3001 aaaactgaaa gctacccgtc aagtcctgtc tgaatacggc aatatgagtt ccgcgtgtgt
     3061 gctgttcatt ctggatgaaa tgcgcaaaaa atctgccgaa gaagctaaag cgaccacggg
     3121 cgaaggtctg gattggggcg tgctgtttgg tttcggtccg ggtctgaccg tcgaaacggt
     3181 cgtgctgcac agtgtgccga tcaaagcggg cggtggcggt tccggcggtg gtggtagtgg
     3241 tggtggtggc tctccgccgc cggccctgcc gccgaaacgt cgtcgctaac tcgagtctgg
     3301 taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag
     3361 cttaattaac ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct
     3421 ctaaacgggt cttgaggggt tttttgctag gatcgagatc gatctcgatc ccgcgaaatt
     3481 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     3541 gtttaacttt aagaaggaga tatacatatg aatccgtcgc cgtctgttac cgaactgcaa
     3601 gtggaaaatg tcacctttac gccgagtctg caaccgccgg gctctaccaa atcgcatttt
     3661 ctgggcggtg caggtgaacg tggcctggaa atcgaaggca aatttgttaa attcaccgct
     3721 attggtgtct atctggaaga aaacgccgtg ccgctgctgg caggcaaatg gaaaggcaaa
     3781 accgccggtg aactgacgga atctgtcgaa tttttccgcg atgtggttac cggcccgttt
     3841 gaaaaattca tgaaagtgac catgatcctg ccgctgacgg gtgcgcagta ttcagaaaaa
     3901 gttgctgaaa attgcatggc gatttggaaa tttttcggca tctacaccga tgcagaagct
     3961 aaagcgattg aaaaatttac ggaagtgttc aaagacgaaa tttttccgcc gggcagctct
     4021 atcctgttca cccaaagttc cggttcgctg acgatttcat tttcgaaaga tggcagcatc
     4081 ccgaaagacg gtgtcgcggt gattgaaaac aatctgctga gcgaagccgt tctggaatct
     4141 atgatcggta aaaacggcgt cagtccggcg gccaaaaaat ccctggccga acgtctgtca
     4201 gcactgctga atgttgcttc cgacaaaatg aaaggcggtg gcggctcagg tggcggtggc
     4261 tctggtggcg gtggttcagg cgtcaaagaa agtctggtgt gactcgagtc tggtaaagaa
     4321 accgctgctg cgaaatttga acgccagcac atggactcgt ctactagtcg cagcttaatt
     4381 aacctaaact gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg
     4441 ggtcttgagg ggttttttgc tagcgaaagg aggagtcgac tatatccgga ttggcgaatg
     4501 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4561 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4621 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4681 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4741 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4801 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4861 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4921 taacgcgaat tttaacaaaa tattaacgtt tacaatttct ggcggcacga tggcatgaga
     4981 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     5041 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     5101 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     5161 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     5221 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     5281 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     5341 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     5401 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     5461 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     5521 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     5581 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     5641 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     5701 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     5761 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     5821 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     5881 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     5941 ctttttcaat catgattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6001 tgaatgtatt tagaaaaata aacaaatagg tcatgaccaa aatcccttaa cgtgagtttt
     6061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     6121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     6181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     6241 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6481 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6721 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6781 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6841 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     6901 ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc
     6961 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     7021 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     7081 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     7141 tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat
     7201 tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat
     7261 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat
     7321 gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
     7381 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
     7441 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
     7501 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
     7561 atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg
     7621 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
     7681 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     7741 agccgggtcc tcaacgacag gagcacgatc atgctagtca tgccccgcgc ccaccggaag
     7801 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg
     7861 agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     7921 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     7981 cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct tcaccgcctg
     8041 gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc gaaaatcctg
     8101 tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt cgtatcccac
     8161 taccgagatg tccgcaccaa cgcgcagccc ggactcggta atggcgcgca ttgcgcccag
     8221 cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat tcagcatttg
     8281 catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg ctatcggctg
     8341 aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg ccgagacaga
     8401 acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca gatgctccac
     8461 gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg tctggtcaga
     8521 gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa tggcatcctg
     8581 gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac
     8641 cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca cgctggcacc
     8701 cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt gcagggccag
     8761 actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg
     8821 gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga
     8881 aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac cggcatactc
     8941 tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac tctcttccgg
     9001 gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg ggatctcgac
     9061 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
     9121 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
     9181 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     9241 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     9301 tgatgccggc cacgatgcgt ccggcgtagc ctaggatcga gatcgatctc gatcccgcga
     9361 aattaatacg actcactata
//
