LOCUS       sequence-135140-        9212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Pc4CL-CmCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..9212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1728..1772
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1825..1872
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1906..1924
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1925..1949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1964..1986
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3260..3304
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3357..3404
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3438..3456
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3457..3481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3496..3518
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      4244..4291
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4335..4790
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4632..4653
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4905..5696,5697..5765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5528..5547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5766..5858)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5939..6527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6428..6447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6681..6698
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6713..6855
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6781..6800)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6941..6963
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(6957..7148)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7720..8802)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8764..8783
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8803..8880)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9086..9105
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9196..9212,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgggagac tgtgtagcac ccaaagaaga ccttattttc cgatcgaaac
      121 tccctgatat ttacatcccg aaacaccttc cgttacatac ttattgtttc gaaaacatct
      181 cgaaagttgg cgacaagtcc tgtttaataa atggcgctac aggcgaaacg ttcacttatt
      241 cccaagttga gctcctttcc aggaaagttg catcagggtt aaacaaactc ggcattcaac
      301 agggcgatac catcatgctt ttgctcccta actcccctga gtattttttc gctttcttag
      361 gcgcatcgta tcgtggtgca atttctacta tggccaatcc gtttttcact tctgctgagg
      421 tgatcaaaca gctcaaagca tcccaagcta agctcataat tacgcaagct tgttacgtag
      481 acaaagtgaa agactacgca gcagagaaaa atatacagat catttgcatc gatgatgctc
      541 ctcaggattg tttacatttc tccaaactta tggaagctga tgaatcagaa atgcctgagg
      601 ttgtgatcaa ttcagacgat gtcgtcgcgt taccttactc atcgggtact acaggactac
      661 cgaaaggtgt tatgttgaca cacaaaggac ttgttactag cgtggcacaa caagttgatg
      721 gagacaatcc gaatttatat atgcatagcg aggatgtgat gatctgcata ttgcctttgt
      781 ttcatattta ttcgcttaac gcggtgttgt gctgtggact cagagcaggg gtgacgatct
      841 tgattatgca gaaatttgat attgtgccat ttttggaact gatacagaaa tataaagtta
      901 caattggacc gtttgtgcca ccaattgtgt tggcaattgc gaaaagtcca gtggtggata
      961 aatatgactt gtcgtcggtg aggacggtta tgtctggagc tgctccgtta gggaaggagc
     1021 ttgaagatgc tgttagagct aagtttccta atgccaaact tggtcaggga tatggaatga
     1081 cagaggcagg gccagtttta gcaatgtgcc tggcgtttgc aaaggaacca tacgagatca
     1141 aatcgggtgc ctgtggaact gttgtgagga atgctgaaat gaaaattgtg gatcctgaga
     1201 ccaacgcctc tcttccacga aaccaacgcg gagagatttg cattcgaggt gaccaaatta
     1261 tgaaaggcta cctcaatgat cctgaatcaa caaggacaac aatagacgaa gaaggctggt
     1321 tgcacacagg agatataggc ttcattgacg acgatgatga gctatttatt gttgatagac
     1381 ttaaggaaat aatcaaatac aaaggcttcc aggttgcccc tgctgaactt gaagctctgc
     1441 tacttactca tcctaccatt tccgatgctg cagttgttcc catgatagat gagaaagcag
     1501 gagaggtgcc tgtggctttt gttgtgagaa caaacggttt caccaccact gaggaagaaa
     1561 tcaagcaatt cgtctcgaaa caggtggtgt tctacaagag aatatttcgt gtattttttg
     1621 ttgatgcaat tccgaaatca ccatctggaa agattcttcg aaaggacttg agagcaaaaa
     1681 tagcatccgg tgatcttccc aaataatagg gtaccctcga gtctggtaaa gaaaccgctg
     1741 ctgcgaaatt tgaacgccag cacatggact cgtctactag tcgcagctta attaacctaa
     1801 actgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     1861 aggggttttt tgctaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta
     1921 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
     1981 aggagatata catatggcta cggtccaaga aatccgcaac gctcaacgcg cagatggtcc
     2041 ggcgacggtc ctggcaatcg gcacggcaac cccggctcat agcgtgaacc aggcagatta
     2101 tccggactat tactttcgta ttaccaaatc tgaacacatg acggaactga aagaaaaatt
     2161 caaacgtatg tgcgataaaa gtatgattaa aaaacgctac atgtacctga ccgaagaaat
     2221 cctgaaagaa aacccgaata tgtgtgccta catggcaccg agcctggatg cgcgccagga
     2281 cattgtggtt gtcgaagttc cgaaactggg taaagaagcg gccaccaaag ccatcaaaga
     2341 atggggccaa ccgaaatcaa aaattacgca cctgatcttt tgcaccacgt cgggtgtgga
     2401 tatgccgggt gcagactatc agctgaccaa actgctgggt ctgcgtccga gcgttaaacg
     2461 ctttatgatg taccagcaag gctgcttcgc aggcggtacg gtcctgcgtc tggctaaaga
     2521 tctggcggaa aacaataaag gtgctcgcgt tctggtggtt tgtagtgaaa ttaccgctgt
     2581 cacgtttcgt ggtccggcgg atacccatct ggactccctg gttggccagg ccctgttcgg
     2641 cgatggtgca gctgcggtta tcgtcggcgc agatccggac acgagtgtgg aacgtccgct
     2701 gtatcagctg gtttcaacct cgcaaacgat tctgccggat tccgacggtg cgatcgatgg
     2761 ccatctgcgc gaagtgggtc tgacctttca cctgctgaaa gacgttccgg gcctgatttc
     2821 aaaaaacatc gaaaaaagcc tgtctgaagc ctttgcaccg gttggtattt cggattggag
     2881 ctctattttc tggatcgcac atccgggcgg tccggcaatc ctggaccagg tggaaagcaa
     2941 actgggtctg aaagaagaaa aactgaaagc tacccgtcaa gtcctgtctg aatacggcaa
     3001 tatgagttcc gcgtgtgtgc tgttcattct ggatgaaatg cgcaaaaaat ctgccgaaga
     3061 agctaaagcg accacgggcg aaggtctgga ttggggcgtg ctgtttggtt tcggtccggg
     3121 tctgaccgtc gaaacggtcg tgctgcacag tgtgccgatc aaagcgggcg gtggcggttc
     3181 cggcggtggt ggtagtggtg gtggtggctc tccgccgccg gccctgccgc cgaaacgtcg
     3241 tcgctaactc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga
     3301 ctcgtctact agtcgcagct taattaacct aaactgctgc caccgctgag caataactag
     3361 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctagga tcgagatcga
     3421 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc
     3481 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc tgcatcaatc
     3541 accgcaatca ctgtggagaa ccttgaatac ccagcggtgg ttacctctcc ggtcaccggc
     3601 aaatcatatt tcctcggtgg cgctggggag agaggattga ccattgaagg aaacttcatc
     3661 aagttcactg ccataggtgt ttatttggaa gatatagcag tggcttcact agctgccaaa
     3721 tggaagggta aatcatctga agagttactt gagacccttg acttttacag agacatcatc
     3781 tcaggtccct ttgaaaagtt aattagaggg tcaaagatta gggaattgag tggtcctgag
     3841 tactcaagga aggttatgga gaactgtgtg gcacacttga aatcagttgg aacttatgga
     3901 gatgcagaag ctgaagctat gcaaaaattt gctgaagctt tcaagcctgt taattttcca
     3961 cctggtgcct ctgttttcta caggcaatca cctaatggaa tattagggct tagtttctct
     4021 ccggatacaa gtataccaga aaaggaggct gcactcatag agaacaaggc agtttcatca
     4081 gcagtgttgg agactatgat cggcgagcac gctgtttccc ctgatcttaa gcgctgttta
     4141 gctgcaagat tacctgcgtt gttgaacgag ggtgctttca agattggaaa ctgaactagt
     4201 cgcagcttaa ttaacctaaa ctgctgccac cgctgagcaa taactagcat aaccccttgg
     4261 ggcctctaaa cgggtcttga ggggtttttt gctagcgaaa ggaggagtcg actatatccg
     4321 gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     4381 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4441 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4501 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4561 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     4621 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     4681 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     4741 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt ctggcggcac
     4801 gatggcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     4861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     4921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     4981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     5641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     5701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     5761 ctcatactct tcctttttca atcatgattg aagcatttat cagggttatt gtctcatgag
     5821 cggatacata tttgaatgta tttagaaaaa taaacaaata ggtcatgacc aaaatccctt
     5881 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     5941 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     6001 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     6061 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     6121 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     6181 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     6241 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     6301 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     6361 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     6421 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     6481 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     6541 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     6601 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     6661 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
     6721 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag
     6781 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
     6841 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     6901 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     6961 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg
     7021 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc
     7081 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt
     7141 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg
     7201 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa
     7261 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag
     7321 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat
     7381 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt
     7441 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag
     7501 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa
     7561 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgctagt catgccccgc
     7621 gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt
     7681 gcctaatgag tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg
     7741 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7801 cgtattgggc gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc
     7861 cttcaccgcc tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag
     7921 gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc
     7981 gtcgtatccc actaccgaga tgtccgcacc aacgcgcagc ccggactcgg taatggcgcg
     8041 cattgcgccc agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc
     8101 attcagcatt tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc
     8161 cgctatcggc tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg
     8221 cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac
     8281 cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg
     8341 tgtctggtca gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc
     8401 aatggcatcc tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag
     8461 aagattgtgc accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac
     8521 cacgctggca cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc
     8581 gtgcagggcc agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg
     8641 ttgtgccacg cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg
     8701 cgttttcgca gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac
     8761 accggcatac tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg
     8821 actctcttcc gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc
     8881 cgggatctcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt
     8941 tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca
     9001 gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga
     9061 agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac
     9121 ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gcctaggatc gagatcgatc
     9181 tcgatcccgc gaaattaata cgactcacta ta
//
