LOCUS       sequence-135141-        9338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Pc4CL-CmCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..9338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1728..1772
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1825..1872
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1906..1924
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1925..1949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1964..1986
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3260..3304
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3357..3404
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3438..3456
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3457..3481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3496..3518
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4273..4317
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4370..4417
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4461..4916
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4758..4779
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5031..5822,5823..5891))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5654..5673
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5892..5984)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6065..6653
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6554..6573
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6807..6824
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6839..6981
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6907..6926)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7067..7089
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7083..7274)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7846..8928)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8890..8909
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8929..9006)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9212..9231
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9322..9338,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgggagac tgtgtagcac ccaaagaaga ccttattttc cgatcgaaac
      121 tccctgatat ttacatcccg aaacaccttc cgttacatac ttattgtttc gaaaacatct
      181 cgaaagttgg cgacaagtcc tgtttaataa atggcgctac aggcgaaacg ttcacttatt
      241 cccaagttga gctcctttcc aggaaagttg catcagggtt aaacaaactc ggcattcaac
      301 agggcgatac catcatgctt ttgctcccta actcccctga gtattttttc gctttcttag
      361 gcgcatcgta tcgtggtgca atttctacta tggccaatcc gtttttcact tctgctgagg
      421 tgatcaaaca gctcaaagca tcccaagcta agctcataat tacgcaagct tgttacgtag
      481 acaaagtgaa agactacgca gcagagaaaa atatacagat catttgcatc gatgatgctc
      541 ctcaggattg tttacatttc tccaaactta tggaagctga tgaatcagaa atgcctgagg
      601 ttgtgatcaa ttcagacgat gtcgtcgcgt taccttactc atcgggtact acaggactac
      661 cgaaaggtgt tatgttgaca cacaaaggac ttgttactag cgtggcacaa caagttgatg
      721 gagacaatcc gaatttatat atgcatagcg aggatgtgat gatctgcata ttgcctttgt
      781 ttcatattta ttcgcttaac gcggtgttgt gctgtggact cagagcaggg gtgacgatct
      841 tgattatgca gaaatttgat attgtgccat ttttggaact gatacagaaa tataaagtta
      901 caattggacc gtttgtgcca ccaattgtgt tggcaattgc gaaaagtcca gtggtggata
      961 aatatgactt gtcgtcggtg aggacggtta tgtctggagc tgctccgtta gggaaggagc
     1021 ttgaagatgc tgttagagct aagtttccta atgccaaact tggtcaggga tatggaatga
     1081 cagaggcagg gccagtttta gcaatgtgcc tggcgtttgc aaaggaacca tacgagatca
     1141 aatcgggtgc ctgtggaact gttgtgagga atgctgaaat gaaaattgtg gatcctgaga
     1201 ccaacgcctc tcttccacga aaccaacgcg gagagatttg cattcgaggt gaccaaatta
     1261 tgaaaggcta cctcaatgat cctgaatcaa caaggacaac aatagacgaa gaaggctggt
     1321 tgcacacagg agatataggc ttcattgacg acgatgatga gctatttatt gttgatagac
     1381 ttaaggaaat aatcaaatac aaaggcttcc aggttgcccc tgctgaactt gaagctctgc
     1441 tacttactca tcctaccatt tccgatgctg cagttgttcc catgatagat gagaaagcag
     1501 gagaggtgcc tgtggctttt gttgtgagaa caaacggttt caccaccact gaggaagaaa
     1561 tcaagcaatt cgtctcgaaa caggtggtgt tctacaagag aatatttcgt gtattttttg
     1621 ttgatgcaat tccgaaatca ccatctggaa agattcttcg aaaggacttg agagcaaaaa
     1681 tagcatccgg tgatcttccc aaataatagg gtaccctcga gtctggtaaa gaaaccgctg
     1741 ctgcgaaatt tgaacgccag cacatggact cgtctactag tcgcagctta attaacctaa
     1801 actgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     1861 aggggttttt tgctaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta
     1921 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
     1981 aggagatata catatggcta cggtccaaga aatccgcaac gctcaacgcg cagatggtcc
     2041 ggcgacggtc ctggcaatcg gcacggcaac cccggctcat agcgtgaacc aggcagatta
     2101 tccggactat tactttcgta ttaccaaatc tgaacacatg acggaactga aagaaaaatt
     2161 caaacgtatg tgcgataaaa gtatgattaa aaaacgctac atgtacctga ccgaagaaat
     2221 cctgaaagaa aacccgaata tgtgtgccta catggcaccg agcctggatg cgcgccagga
     2281 cattgtggtt gtcgaagttc cgaaactggg taaagaagcg gccaccaaag ccatcaaaga
     2341 atggggccaa ccgaaatcaa aaattacgca cctgatcttt tgcaccacgt cgggtgtgga
     2401 tatgccgggt gcagactatc agctgaccaa actgctgggt ctgcgtccga gcgttaaacg
     2461 ctttatgatg taccagcaag gctgcttcgc aggcggtacg gtcctgcgtc tggctaaaga
     2521 tctggcggaa aacaataaag gtgctcgcgt tctggtggtt tgtagtgaaa ttaccgctgt
     2581 cacgtttcgt ggtccggcgg atacccatct ggactccctg gttggccagg ccctgttcgg
     2641 cgatggtgca gctgcggtta tcgtcggcgc agatccggac acgagtgtgg aacgtccgct
     2701 gtatcagctg gtttcaacct cgcaaacgat tctgccggat tccgacggtg cgatcgatgg
     2761 ccatctgcgc gaagtgggtc tgacctttca cctgctgaaa gacgttccgg gcctgatttc
     2821 aaaaaacatc gaaaaaagcc tgtctgaagc ctttgcaccg gttggtattt cggattggag
     2881 ctctattttc tggatcgcac atccgggcgg tccggcaatc ctggaccagg tggaaagcaa
     2941 actgggtctg aaagaagaaa aactgaaagc tacccgtcaa gtcctgtctg aatacggcaa
     3001 tatgagttcc gcgtgtgtgc tgttcattct ggatgaaatg cgcaaaaaat ctgccgaaga
     3061 agctaaagcg accacgggcg aaggtctgga ttggggcgtg ctgtttggtt tcggtccggg
     3121 tctgaccgtc gaaacggtcg tgctgcacag tgtgccgatc aaagcgggcg gtggcggttc
     3181 cggcggtggt ggtagtggtg gtggtggctc tccgccgccg gccctgccgc cgaaacgtcg
     3241 tcgctaactc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc agcacatgga
     3301 ctcgtctact agtcgcagct taattaacct aaactgctgc caccgctgag caataactag
     3361 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctagga tcgagatcga
     3421 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc
     3481 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgaa tccgtcgccg
     3541 tctgttaccg aactgcaagt ggaaaatgtc acctttacgc cgagtctgca accgccgggc
     3601 tctaccaaat cgcattttct gggcggtgca ggtgaacgtg gcctggaaat cgaaggcaaa
     3661 tttgttaaat tcaccgctat tggtgtctat ctggaagaaa acgccgtgcc gctgctggca
     3721 ggcaaatgga aaggcaaaac cgccggtgaa ctgacggaat ctgtcgaatt tttccgcgat
     3781 gtggttaccg gcccgtttga aaaattcatg aaagtgacca tgatcctgcc gctgacgggt
     3841 gcgcagtatt cagaaaaagt tgctgaaaat tgcatggcga tttggaaatt tttcggcatc
     3901 tacaccgatg cagaagctaa agcgattgaa aaatttacgg aagtgttcaa agacgaaatt
     3961 tttccgccgg gcagctctat cctgttcacc caaagttccg gttcgctgac gatttcattt
     4021 tcgaaagatg gcagcatccc gaaagacggt gtcgcggtga ttgaaaacaa tctgctgagc
     4081 gaagccgttc tggaatctat gatcggtaaa aacggcgtca gtccggcggc caaaaaatcc
     4141 ctggccgaac gtctgtcagc actgctgaat gttgcttccg acaaaatgaa aggcggtggc
     4201 ggctcaggtg gcggtggctc tggtggcggt ggttcaggcg tcaaagaaag tctggtgtga
     4261 ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat ggactcgtct
     4321 actagtcgca gcttaattaa cctaaactgc tgccaccgct gagcaataac tagcataacc
     4381 ccttggggcc tctaaacggg tcttgagggg ttttttgcta gcgaaaggag gagtcgacta
     4441 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     4501 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     4561 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     4621 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     4681 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     4741 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     4801 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     4861 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttctgg
     4921 cggcacgatg gcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     4981 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5041 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5101 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5161 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5221 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5281 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5341 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5401 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5461 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     5521 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     5581 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     5641 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     5701 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     5761 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     5821 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     5881 tgaatactca tactcttcct ttttcaatca tgattgaagc atttatcagg gttattgtct
     5941 catgagcgga tacatatttg aatgtattta gaaaaataaa caaataggtc atgaccaaaa
     6001 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     6061 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     6121 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     6181 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
     6241 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     6301 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     6361 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     6421 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6481 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6541 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6601 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6661 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     6721 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     6781 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6841 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac
     6901 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
     6961 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     7021 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     7081 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca
     7141 gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt
     7201 ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt
     7261 tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata
     7321 ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta
     7381 ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc
     7441 actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag
     7501 cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc
     7561 agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt
     7621 ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta
     7681 aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gctagtcatg
     7741 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagat
     7801 cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact gcccgctttc
     7861 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7921 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     7981 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     8041 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     8101 ggtatcgtcg tatcccacta ccgagatgtc cgcaccaacg cgcagcccgg actcggtaat
     8161 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     8221 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     8281 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     8341 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     8401 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     8461 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     8521 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
     8581 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     8641 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     8701 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     8761 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     8821 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     8881 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     8941 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     9001 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     9061 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     9121 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     9181 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     9241 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagcct aggatcgaga
     9301 tcgatctcga tcccgcgaaa ttaatacgac tcactata
//
