LOCUS       sequence-135142-        9347 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9347)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9347)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Vv4CL-PhCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..9347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1938..1982
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2035..2082
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2116..2134
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2135..2159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2174..2196
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3395..3439
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3492..3539
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3573..3591
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3592..3616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3631..3653
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      4379..4426
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4470..4925
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4767..4788
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5040..5831,5832..5900))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5663..5682
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5901..5993)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6074..6662
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6563..6582
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6816..6833
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6848..6990
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6916..6935)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7076..7098
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7092..7283)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7855..8937)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8899..8918
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8938..9015)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9221..9240
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9331..9347,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgattagt attgaaacgc aaaacccgga tgttagcaac ctggacacct
      121 cgcactctat tccgaaaatg gcaaaccgta ttgatgacca tgtgtttcgt tctaaactgc
      181 cggaaattcc gatcagtaac catctgccgc tgcacacgta ttgcttcgaa aattactcgc
      241 agtttgcaga ccgtccgtgt ctgattgttg gctcgacgaa caaaacctat agcttcgctg
      301 aaacccatct gatctctcgc aaagtgggcg caggttttgc tcacctgggt ctgaaacagg
      361 gcgatgtggt tatgattctg ctgcaaaatt gcgcggaatt tgccttcagc tttctgggtg
      421 cgtctatggt tggcgccgtc accacgaccg caaacccgtt ctacacgtcc gcggaaatct
      481 tcaaacagct gaacgcatca aaagctaaaa tcgtcgtgac ccaggcgcaa tatgtggata
      541 aactgcgcga ctacccggat ggtcaagttg ccaaaattgg cgaaggtttc acggtcatta
      601 ccatcgatga cccgccggaa aactgtatgc attttagtgt tgtctccgaa gcgaacgaaa
      661 gcgaactgcc ggaagtctca attaattcgg atgacccggt ggccctgccg tttagctctg
      721 gtacgaccgg cctgccgaaa ggcgtggttc tgacgcacaa atcactgatc acctcggtcg
      781 cccagcaagt ggatggtgaa aacccgaatc tgcatctgac cccggatgac gtcgtgctgt
      841 gcgtgctgcc gctgttccac atttatagcc tgaactctgt tctgctgtgt agtctgcgtg
      901 caggtgcagc agtgctgctg atgcagaaat ttgaaattgg taccctgctg gaactgatcc
      961 aacgttaccg cgtgagcgtt gcagctgttg tcccgccgct ggttctggca ctggctaaaa
     1021 atccgatggt ggaatcgttt gatctgagtt ccatccgtgt ggttctgagc ggtgcagcac
     1081 cgctgggcaa agaactggaa gcagctctgc gttcccgcgt tccgcaggca gtcctgggcc
     1141 aaggttatgg catgacggaa gcaggcccgg tgctgtcaat gtgcctgggt ttcgctaaac
     1201 agccgtttcc gacgaaatca ggttcgtgtg gcaccgtcgt gcgtaacgcg gaactgaaag
     1261 ttgtggatcc ggaaaccggt tgctccctgg gccgtaatca gccgggtgaa atttgtatcc
     1321 gcggccagca aattatgaaa ggttatctga atgatccgga agcgacggcc tctaccattg
     1381 acgttgatgg ctggctgcat accggtgaca tcggctacgt ggatgacgat gaagaagtgt
     1441 tcattgttga tcgcgtcaaa gaactgatca aattcaaagg ttttcaggtt ccgccggcag
     1501 aactggaagc tctgctggtg tctcacccgt ccattgccga tgcggccgtg gttccgcaaa
     1561 aagacgatgt tgctggcgaa gtcccggtgg cgttcgtcgt gcgttctaac ggttttgaac
     1621 tgaccgaaga agcagtgaaa gaattcatca gtaaacaggt tgtcttttat aaacgcctgc
     1681 ataaagtgta ctttgttcac gcgattccga aaagcccgtc tggcaaaatc ctgcgtaaag
     1741 atctgcgcgc gaaactggcc gaaaaaaccc cggaaccgaa cggcggtggc ggtagtggcg
     1801 gtggcggttc cggcggtggc ggttcactgg tgggtgccct gatgcatgtt atgcagaaac
     1861 gtagccgtgc tatccactcc tcagatgaag gtgaagacca ggcaggtgac gaagatgaag
     1921 actaactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact
     1981 cgtctactag tcgcagctta attaacctaa actgctgcca ccgctgagca ataactagca
     2041 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctaggatc gagatcgatc
     2101 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     2161 ctctagaaat aattttgttt aactttaaga aggagatata catatggtga cagtcgagga
     2221 gtatcgtaag gcacaacgtg ctgaaggtcc agccactgtc atggccattg gaacagccac
     2281 accttcaaac tgtgttgatc aaagcactta ccctgatttt tattttcgta tcactaacag
     2341 tgagcacaag actgatctta aggagaaatt taagcgcatg tgtgaaaaat caatgattaa
     2401 gaaaaggtac atgcacttaa cagaggaaat cttgaaagag aatcctagta tgtgtgaata
     2461 catggcacct tctcttgatg ctaggcaaga catagtggtg gttgaagtgc ccaaacttgg
     2521 caaagaggca gctcaaaagg ccatcaagga atggggccag cccaagtcca aaattaccca
     2581 tttggtcttt tgcacaacca gtggtgtgga catgcctggg tgtgactatc aactcactaa
     2641 gctacttggg cttcgtccat cggtcaagag gcttatgatg taccaacaag gttgctttgc
     2701 tggtggcacg gttcttcggt tagccaagga cttggctgaa aacaacaagg gcgctcgagt
     2761 ccttgttgtt tgttcagaaa tcaccgcggt cactttccgt gggccaaatg atactcattt
     2821 ggatagttta gttggccaag cactttttgg tgatggggca ggcgcgatca ttataggttc
     2881 tgatccaatt ccaggggtcg aaaggccttt gttcgagctc gtttcagcag cccaaactct
     2941 tctcccagat agccatggtg ctattgatgg ccatctccgt gaagttgggc ttacattcca
     3001 cttactcaaa gatgttcctg ggctgatctc aaaaaatatt gagaagagcc ttgaggaagc
     3061 attcaaacct ttgggcattt ctgattggaa ctctctattc tggattgctc atccaggtgg
     3121 gcctgcaatt ttggaccaag ttgaaataaa gttgggccta aagcccgaga aacttaaggc
     3181 tacaaggaat gtgttaagta actatggtaa catgtcaagt gcttgtgtac tgtttatttt
     3241 ggatgaaatg agaaaggcct cagccaaaga aggtttagga actactggtg aagggcttga
     3301 gtggggtgtt ctttttggat ttgggcctgg gctaacagtt gagactgttg tcctccacag
     3361 tgttgctact taatagggta ccctcgagtc tggtaaagaa accgctgctg cgaaatttga
     3421 acgccagcac atggactcgt ctactagtcg cagcttaatt aacctaaact gctgccaccg
     3481 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     3541 taggatcgag atcgatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg
     3601 agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat
     3661 atggctgcat caatcaccgc aatcactgtg gagaaccttg aatacccagc ggtggttacc
     3721 tctccggtca ccggcaaatc atatttcctc ggtggcgctg gggagagagg attgaccatt
     3781 gaaggaaact tcatcaagtt cactgccata ggtgtttatt tggaagatat agcagtggct
     3841 tcactagctg ccaaatggaa gggtaaatca tctgaagagt tacttgagac ccttgacttt
     3901 tacagagaca tcatctcagg tccctttgaa aagttaatta gagggtcaaa gattagggaa
     3961 ttgagtggtc ctgagtactc aaggaaggtt atggagaact gtgtggcaca cttgaaatca
     4021 gttggaactt atggagatgc agaagctgaa gctatgcaaa aatttgctga agctttcaag
     4081 cctgttaatt ttccacctgg tgcctctgtt ttctacaggc aatcacctaa tggaatatta
     4141 gggcttagtt tctctccgga tacaagtata ccagaaaagg aggctgcact catagagaac
     4201 aaggcagttt catcagcagt gttggagact atgatcggcg agcacgctgt ttcccctgat
     4261 cttaagcgct gtttagctgc aagattacct gcgttgttga acgagggtgc tttcaagatt
     4321 ggaaactgaa ctagtcgcag cttaattaac ctaaactgct gccaccgctg agcaataact
     4381 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctag cgaaaggagg
     4441 agtcgactat atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     4501 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4561 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4621 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4681 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4741 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4801 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4861 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
     4921 aatttctggc ggcacgatgg catgagatta tcaaaaagga tcttcaccta gatcctttta
     4981 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     5041 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     5101 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     5161 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     5221 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     5281 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     5341 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     5401 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     5461 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     5521 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     5581 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     5641 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     5701 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     5761 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     5821 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     5881 cggaaatgtt gaatactcat actcttcctt tttcaatcat gattgaagca tttatcaggg
     5941 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggtca
     6001 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6061 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6121 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6181 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     6241 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6301 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6361 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6421 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6481 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6541 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6601 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6661 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6721 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6781 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6841 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
     6901 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     6961 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     7021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     7081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     7141 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     7201 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     7261 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     7321 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     7381 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     7441 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     7501 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     7561 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
     7621 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     7681 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     7741 ctagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     7801 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
     7861 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     7921 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg
     7981 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     8041 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     8101 gctgtcttcg gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc gcagcccgga
     8161 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     8221 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     8281 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     8341 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     8401 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat
     8461 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     8521 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     8581 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     8641 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     8701 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     8761 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     8821 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     8881 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     8941 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
     9001 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
     9061 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
     9121 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     9181 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     9241 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagccta
     9301 ggatcgagat cgatctcgat cccgcgaaat taatacgact cactata
//
