LOCUS       sequence-135143-        9473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Vv4CL-PhCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..9473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1938..1982
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2035..2082
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2116..2134
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2135..2159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2174..2196
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3395..3439
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3492..3539
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3573..3591
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3592..3616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3631..3653
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4408..4452
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4505..4552
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4596..5051
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4893..4914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5166..5957,5958..6026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5789..5808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6027..6119)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6200..6788
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6689..6708
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6942..6959
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6974..7116
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7042..7061)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7202..7224
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7218..7409)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7981..9063)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9025..9044
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9064..9141)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9347..9366
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9457..9473,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgattagt attgaaacgc aaaacccgga tgttagcaac ctggacacct
      121 cgcactctat tccgaaaatg gcaaaccgta ttgatgacca tgtgtttcgt tctaaactgc
      181 cggaaattcc gatcagtaac catctgccgc tgcacacgta ttgcttcgaa aattactcgc
      241 agtttgcaga ccgtccgtgt ctgattgttg gctcgacgaa caaaacctat agcttcgctg
      301 aaacccatct gatctctcgc aaagtgggcg caggttttgc tcacctgggt ctgaaacagg
      361 gcgatgtggt tatgattctg ctgcaaaatt gcgcggaatt tgccttcagc tttctgggtg
      421 cgtctatggt tggcgccgtc accacgaccg caaacccgtt ctacacgtcc gcggaaatct
      481 tcaaacagct gaacgcatca aaagctaaaa tcgtcgtgac ccaggcgcaa tatgtggata
      541 aactgcgcga ctacccggat ggtcaagttg ccaaaattgg cgaaggtttc acggtcatta
      601 ccatcgatga cccgccggaa aactgtatgc attttagtgt tgtctccgaa gcgaacgaaa
      661 gcgaactgcc ggaagtctca attaattcgg atgacccggt ggccctgccg tttagctctg
      721 gtacgaccgg cctgccgaaa ggcgtggttc tgacgcacaa atcactgatc acctcggtcg
      781 cccagcaagt ggatggtgaa aacccgaatc tgcatctgac cccggatgac gtcgtgctgt
      841 gcgtgctgcc gctgttccac atttatagcc tgaactctgt tctgctgtgt agtctgcgtg
      901 caggtgcagc agtgctgctg atgcagaaat ttgaaattgg taccctgctg gaactgatcc
      961 aacgttaccg cgtgagcgtt gcagctgttg tcccgccgct ggttctggca ctggctaaaa
     1021 atccgatggt ggaatcgttt gatctgagtt ccatccgtgt ggttctgagc ggtgcagcac
     1081 cgctgggcaa agaactggaa gcagctctgc gttcccgcgt tccgcaggca gtcctgggcc
     1141 aaggttatgg catgacggaa gcaggcccgg tgctgtcaat gtgcctgggt ttcgctaaac
     1201 agccgtttcc gacgaaatca ggttcgtgtg gcaccgtcgt gcgtaacgcg gaactgaaag
     1261 ttgtggatcc ggaaaccggt tgctccctgg gccgtaatca gccgggtgaa atttgtatcc
     1321 gcggccagca aattatgaaa ggttatctga atgatccgga agcgacggcc tctaccattg
     1381 acgttgatgg ctggctgcat accggtgaca tcggctacgt ggatgacgat gaagaagtgt
     1441 tcattgttga tcgcgtcaaa gaactgatca aattcaaagg ttttcaggtt ccgccggcag
     1501 aactggaagc tctgctggtg tctcacccgt ccattgccga tgcggccgtg gttccgcaaa
     1561 aagacgatgt tgctggcgaa gtcccggtgg cgttcgtcgt gcgttctaac ggttttgaac
     1621 tgaccgaaga agcagtgaaa gaattcatca gtaaacaggt tgtcttttat aaacgcctgc
     1681 ataaagtgta ctttgttcac gcgattccga aaagcccgtc tggcaaaatc ctgcgtaaag
     1741 atctgcgcgc gaaactggcc gaaaaaaccc cggaaccgaa cggcggtggc ggtagtggcg
     1801 gtggcggttc cggcggtggc ggttcactgg tgggtgccct gatgcatgtt atgcagaaac
     1861 gtagccgtgc tatccactcc tcagatgaag gtgaagacca ggcaggtgac gaagatgaag
     1921 actaactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact
     1981 cgtctactag tcgcagctta attaacctaa actgctgcca ccgctgagca ataactagca
     2041 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctaggatc gagatcgatc
     2101 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     2161 ctctagaaat aattttgttt aactttaaga aggagatata catatggtga cagtcgagga
     2221 gtatcgtaag gcacaacgtg ctgaaggtcc agccactgtc atggccattg gaacagccac
     2281 accttcaaac tgtgttgatc aaagcactta ccctgatttt tattttcgta tcactaacag
     2341 tgagcacaag actgatctta aggagaaatt taagcgcatg tgtgaaaaat caatgattaa
     2401 gaaaaggtac atgcacttaa cagaggaaat cttgaaagag aatcctagta tgtgtgaata
     2461 catggcacct tctcttgatg ctaggcaaga catagtggtg gttgaagtgc ccaaacttgg
     2521 caaagaggca gctcaaaagg ccatcaagga atggggccag cccaagtcca aaattaccca
     2581 tttggtcttt tgcacaacca gtggtgtgga catgcctggg tgtgactatc aactcactaa
     2641 gctacttggg cttcgtccat cggtcaagag gcttatgatg taccaacaag gttgctttgc
     2701 tggtggcacg gttcttcggt tagccaagga cttggctgaa aacaacaagg gcgctcgagt
     2761 ccttgttgtt tgttcagaaa tcaccgcggt cactttccgt gggccaaatg atactcattt
     2821 ggatagttta gttggccaag cactttttgg tgatggggca ggcgcgatca ttataggttc
     2881 tgatccaatt ccaggggtcg aaaggccttt gttcgagctc gtttcagcag cccaaactct
     2941 tctcccagat agccatggtg ctattgatgg ccatctccgt gaagttgggc ttacattcca
     3001 cttactcaaa gatgttcctg ggctgatctc aaaaaatatt gagaagagcc ttgaggaagc
     3061 attcaaacct ttgggcattt ctgattggaa ctctctattc tggattgctc atccaggtgg
     3121 gcctgcaatt ttggaccaag ttgaaataaa gttgggccta aagcccgaga aacttaaggc
     3181 tacaaggaat gtgttaagta actatggtaa catgtcaagt gcttgtgtac tgtttatttt
     3241 ggatgaaatg agaaaggcct cagccaaaga aggtttagga actactggtg aagggcttga
     3301 gtggggtgtt ctttttggat ttgggcctgg gctaacagtt gagactgttg tcctccacag
     3361 tgttgctact taatagggta ccctcgagtc tggtaaagaa accgctgctg cgaaatttga
     3421 acgccagcac atggactcgt ctactagtcg cagcttaatt aacctaaact gctgccaccg
     3481 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     3541 taggatcgag atcgatctcg atcccgcgaa attaatacga ctcactatag gggaattgtg
     3601 agcggataac aattcccctc tagaaataat tttgtttaac tttaagaagg agatatacat
     3661 atgaatccgt cgccgtctgt taccgaactg caagtggaaa atgtcacctt tacgccgagt
     3721 ctgcaaccgc cgggctctac caaatcgcat tttctgggcg gtgcaggtga acgtggcctg
     3781 gaaatcgaag gcaaatttgt taaattcacc gctattggtg tctatctgga agaaaacgcc
     3841 gtgccgctgc tggcaggcaa atggaaaggc aaaaccgccg gtgaactgac ggaatctgtc
     3901 gaatttttcc gcgatgtggt taccggcccg tttgaaaaat tcatgaaagt gaccatgatc
     3961 ctgccgctga cgggtgcgca gtattcagaa aaagttgctg aaaattgcat ggcgatttgg
     4021 aaatttttcg gcatctacac cgatgcagaa gctaaagcga ttgaaaaatt tacggaagtg
     4081 ttcaaagacg aaatttttcc gccgggcagc tctatcctgt tcacccaaag ttccggttcg
     4141 ctgacgattt cattttcgaa agatggcagc atcccgaaag acggtgtcgc ggtgattgaa
     4201 aacaatctgc tgagcgaagc cgttctggaa tctatgatcg gtaaaaacgg cgtcagtccg
     4261 gcggccaaaa aatccctggc cgaacgtctg tcagcactgc tgaatgttgc ttccgacaaa
     4321 atgaaaggcg gtggcggctc aggtggcggt ggctctggtg gcggtggttc aggcgtcaaa
     4381 gaaagtctgg tgtgactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag
     4441 cacatggact cgtctactag tcgcagctta attaacctaa actgctgcca ccgctgagca
     4501 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctagcgaa
     4561 aggaggagtc gactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
     4621 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4681 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4741 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4801 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4861 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4921 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4981 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5041 gtttacaatt tctggcggca cgatggcatg agattatcaa aaaggatctt cacctagatc
     5101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     5161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5461 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5761 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatcatgatt gaagcattta
     6061 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     6121 aggtcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6181 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6241 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6301 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6361 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6421 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6481 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6541 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6601 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6661 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6721 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6781 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6841 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6901 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6961 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7021 gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac
     7081 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct
     7141 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7201 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg
     7261 cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg
     7321 tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg
     7381 ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc
     7441 atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat
     7501 gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg
     7561 gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt
     7621 ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct
     7681 gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct
     7741 caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca
     7801 ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg
     7861 atcatgctag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     7921 ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat tgcgttgcgc
     7981 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     8041 cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga
     8101 gacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc
     8161 cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata
     8221 acatgagctg tcttcggtat cgtcgtatcc cactaccgag atgtccgcac caacgcgcag
     8281 cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat
     8341 cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc
     8401 actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg
     8461 ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat
     8521 ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga
     8581 gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt
     8641 agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag
     8701 cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct
     8761 tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc
     8821 cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa
     8881 cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat
     8941 cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg
     9001 ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt
     9061 cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt
     9121 tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta
     9181 ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat
     9241 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     9301 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     9361 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     9421 agcctaggat cgagatcgat ctcgatcccg cgaaattaat acgactcact ata
//
