LOCUS       sequence-135144-        9422 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Vv4CL-CmCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..9422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1938..1982
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2035..2082
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2116..2134
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2135..2159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2174..2196
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3470..3514
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3567..3614
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3648..3666
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3667..3691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3706..3728
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      4454..4501
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4545..5000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4842..4863
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5115..5906,5907..5975))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5738..5757
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5976..6068)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6149..6737
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6638..6657
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6891..6908
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6923..7065
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6991..7010)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7151..7173
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7167..7358)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7930..9012)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8974..8993
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9013..9090)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9296..9315
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9406..9422,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgattagt attgaaacgc aaaacccgga tgttagcaac ctggacacct
      121 cgcactctat tccgaaaatg gcaaaccgta ttgatgacca tgtgtttcgt tctaaactgc
      181 cggaaattcc gatcagtaac catctgccgc tgcacacgta ttgcttcgaa aattactcgc
      241 agtttgcaga ccgtccgtgt ctgattgttg gctcgacgaa caaaacctat agcttcgctg
      301 aaacccatct gatctctcgc aaagtgggcg caggttttgc tcacctgggt ctgaaacagg
      361 gcgatgtggt tatgattctg ctgcaaaatt gcgcggaatt tgccttcagc tttctgggtg
      421 cgtctatggt tggcgccgtc accacgaccg caaacccgtt ctacacgtcc gcggaaatct
      481 tcaaacagct gaacgcatca aaagctaaaa tcgtcgtgac ccaggcgcaa tatgtggata
      541 aactgcgcga ctacccggat ggtcaagttg ccaaaattgg cgaaggtttc acggtcatta
      601 ccatcgatga cccgccggaa aactgtatgc attttagtgt tgtctccgaa gcgaacgaaa
      661 gcgaactgcc ggaagtctca attaattcgg atgacccggt ggccctgccg tttagctctg
      721 gtacgaccgg cctgccgaaa ggcgtggttc tgacgcacaa atcactgatc acctcggtcg
      781 cccagcaagt ggatggtgaa aacccgaatc tgcatctgac cccggatgac gtcgtgctgt
      841 gcgtgctgcc gctgttccac atttatagcc tgaactctgt tctgctgtgt agtctgcgtg
      901 caggtgcagc agtgctgctg atgcagaaat ttgaaattgg taccctgctg gaactgatcc
      961 aacgttaccg cgtgagcgtt gcagctgttg tcccgccgct ggttctggca ctggctaaaa
     1021 atccgatggt ggaatcgttt gatctgagtt ccatccgtgt ggttctgagc ggtgcagcac
     1081 cgctgggcaa agaactggaa gcagctctgc gttcccgcgt tccgcaggca gtcctgggcc
     1141 aaggttatgg catgacggaa gcaggcccgg tgctgtcaat gtgcctgggt ttcgctaaac
     1201 agccgtttcc gacgaaatca ggttcgtgtg gcaccgtcgt gcgtaacgcg gaactgaaag
     1261 ttgtggatcc ggaaaccggt tgctccctgg gccgtaatca gccgggtgaa atttgtatcc
     1321 gcggccagca aattatgaaa ggttatctga atgatccgga agcgacggcc tctaccattg
     1381 acgttgatgg ctggctgcat accggtgaca tcggctacgt ggatgacgat gaagaagtgt
     1441 tcattgttga tcgcgtcaaa gaactgatca aattcaaagg ttttcaggtt ccgccggcag
     1501 aactggaagc tctgctggtg tctcacccgt ccattgccga tgcggccgtg gttccgcaaa
     1561 aagacgatgt tgctggcgaa gtcccggtgg cgttcgtcgt gcgttctaac ggttttgaac
     1621 tgaccgaaga agcagtgaaa gaattcatca gtaaacaggt tgtcttttat aaacgcctgc
     1681 ataaagtgta ctttgttcac gcgattccga aaagcccgtc tggcaaaatc ctgcgtaaag
     1741 atctgcgcgc gaaactggcc gaaaaaaccc cggaaccgaa cggcggtggc ggtagtggcg
     1801 gtggcggttc cggcggtggc ggttcactgg tgggtgccct gatgcatgtt atgcagaaac
     1861 gtagccgtgc tatccactcc tcagatgaag gtgaagacca ggcaggtgac gaagatgaag
     1921 actaactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact
     1981 cgtctactag tcgcagctta attaacctaa actgctgcca ccgctgagca ataactagca
     2041 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctaggatc gagatcgatc
     2101 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     2161 ctctagaaat aattttgttt aactttaaga aggagatata catatggcta cggtccaaga
     2221 aatccgcaac gctcaacgcg cagatggtcc ggcgacggtc ctggcaatcg gcacggcaac
     2281 cccggctcat agcgtgaacc aggcagatta tccggactat tactttcgta ttaccaaatc
     2341 tgaacacatg acggaactga aagaaaaatt caaacgtatg tgcgataaaa gtatgattaa
     2401 aaaacgctac atgtacctga ccgaagaaat cctgaaagaa aacccgaata tgtgtgccta
     2461 catggcaccg agcctggatg cgcgccagga cattgtggtt gtcgaagttc cgaaactggg
     2521 taaagaagcg gccaccaaag ccatcaaaga atggggccaa ccgaaatcaa aaattacgca
     2581 cctgatcttt tgcaccacgt cgggtgtgga tatgccgggt gcagactatc agctgaccaa
     2641 actgctgggt ctgcgtccga gcgttaaacg ctttatgatg taccagcaag gctgcttcgc
     2701 aggcggtacg gtcctgcgtc tggctaaaga tctggcggaa aacaataaag gtgctcgcgt
     2761 tctggtggtt tgtagtgaaa ttaccgctgt cacgtttcgt ggtccggcgg atacccatct
     2821 ggactccctg gttggccagg ccctgttcgg cgatggtgca gctgcggtta tcgtcggcgc
     2881 agatccggac acgagtgtgg aacgtccgct gtatcagctg gtttcaacct cgcaaacgat
     2941 tctgccggat tccgacggtg cgatcgatgg ccatctgcgc gaagtgggtc tgacctttca
     3001 cctgctgaaa gacgttccgg gcctgatttc aaaaaacatc gaaaaaagcc tgtctgaagc
     3061 ctttgcaccg gttggtattt cggattggag ctctattttc tggatcgcac atccgggcgg
     3121 tccggcaatc ctggaccagg tggaaagcaa actgggtctg aaagaagaaa aactgaaagc
     3181 tacccgtcaa gtcctgtctg aatacggcaa tatgagttcc gcgtgtgtgc tgttcattct
     3241 ggatgaaatg cgcaaaaaat ctgccgaaga agctaaagcg accacgggcg aaggtctgga
     3301 ttggggcgtg ctgtttggtt tcggtccggg tctgaccgtc gaaacggtcg tgctgcacag
     3361 tgtgccgatc aaagcgggcg gtggcggttc cggcggtggt ggtagtggtg gtggtggctc
     3421 tccgccgccg gccctgccgc cgaaacgtcg tcgctaactc gagtctggta aagaaaccgc
     3481 tgctgcgaaa tttgaacgcc agcacatgga ctcgtctact agtcgcagct taattaacct
     3541 aaactgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     3601 tgaggggttt tttgctagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
     3661 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
     3721 gaaggagata tacatatggc tgcatcaatc accgcaatca ctgtggagaa ccttgaatac
     3781 ccagcggtgg ttacctctcc ggtcaccggc aaatcatatt tcctcggtgg cgctggggag
     3841 agaggattga ccattgaagg aaacttcatc aagttcactg ccataggtgt ttatttggaa
     3901 gatatagcag tggcttcact agctgccaaa tggaagggta aatcatctga agagttactt
     3961 gagacccttg acttttacag agacatcatc tcaggtccct ttgaaaagtt aattagaggg
     4021 tcaaagatta gggaattgag tggtcctgag tactcaagga aggttatgga gaactgtgtg
     4081 gcacacttga aatcagttgg aacttatgga gatgcagaag ctgaagctat gcaaaaattt
     4141 gctgaagctt tcaagcctgt taattttcca cctggtgcct ctgttttcta caggcaatca
     4201 cctaatggaa tattagggct tagtttctct ccggatacaa gtataccaga aaaggaggct
     4261 gcactcatag agaacaaggc agtttcatca gcagtgttgg agactatgat cggcgagcac
     4321 gctgtttccc ctgatcttaa gcgctgttta gctgcaagat tacctgcgtt gttgaacgag
     4381 ggtgctttca agattggaaa ctgaactagt cgcagcttaa ttaacctaaa ctgctgccac
     4441 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     4501 gctagcgaaa ggaggagtcg actatatccg gattggcgaa tgggacgcgc cctgtagcgg
     4561 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4621 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     4681 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     4741 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     4801 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     4861 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     4921 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     4981 aatattaacg tttacaattt ctggcggcac gatggcatga gattatcaaa aaggatcttc
     5041 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5101 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5161 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5221 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5281 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     5341 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     5401 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     5461 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     5521 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     5581 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     5641 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     5701 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     5761 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     5821 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     5881 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     5941 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atcatgattg
     6001 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     6061 taaacaaata ggtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6121 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6181 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6241 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6301 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6361 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6421 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6481 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6541 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6601 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6661 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6721 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     6781 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     6841 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     6901 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     6961 tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     7021 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     7081 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     7141 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     7201 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt
     7261 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag
     7321 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga
     7381 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt
     7441 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg
     7501 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat
     7561 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg
     7621 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat
     7681 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc
     7741 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac
     7801 aggagcacga tcatgctagt catgccccgc gcccaccgga aggagctgac tgggttgaag
     7861 gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt
     7921 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     7981 atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt
     8041 caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag
     8101 caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg
     8161 cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tgtccgcacc
     8221 aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc
     8281 aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc
     8341 ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag
     8401 atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa
     8461 cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc
     8521 ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc
     8581 cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt
     8641 aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc
     8701 gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga
     8761 tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc
     8821 aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag
     8881 ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt
     8941 caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt
     9001 tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc
     9061 gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact
     9121 cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga
     9181 atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac
     9241 ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga
     9301 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc
     9361 gtccggcgta gcctaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta
     9421 ta
//
