LOCUS       sequence-135145-        9548 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9548)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Vv4CL-CmCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..9548
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1938..1982
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      2035..2082
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        2116..2134
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2135..2159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2174..2196
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3470..3514
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3567..3614
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3648..3666
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3667..3691
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3706..3728
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4483..4527
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4580..4627
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4671..5126
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4968..4989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5241..6032,6033..6101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5864..5883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6102..6194)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6275..6863
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6764..6783
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7017..7034
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7049..7191
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7117..7136)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7277..7299
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7293..7484)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8056..9138)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9100..9119
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9139..9216)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9422..9441
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9532..9548,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgattagt attgaaacgc aaaacccgga tgttagcaac ctggacacct
      121 cgcactctat tccgaaaatg gcaaaccgta ttgatgacca tgtgtttcgt tctaaactgc
      181 cggaaattcc gatcagtaac catctgccgc tgcacacgta ttgcttcgaa aattactcgc
      241 agtttgcaga ccgtccgtgt ctgattgttg gctcgacgaa caaaacctat agcttcgctg
      301 aaacccatct gatctctcgc aaagtgggcg caggttttgc tcacctgggt ctgaaacagg
      361 gcgatgtggt tatgattctg ctgcaaaatt gcgcggaatt tgccttcagc tttctgggtg
      421 cgtctatggt tggcgccgtc accacgaccg caaacccgtt ctacacgtcc gcggaaatct
      481 tcaaacagct gaacgcatca aaagctaaaa tcgtcgtgac ccaggcgcaa tatgtggata
      541 aactgcgcga ctacccggat ggtcaagttg ccaaaattgg cgaaggtttc acggtcatta
      601 ccatcgatga cccgccggaa aactgtatgc attttagtgt tgtctccgaa gcgaacgaaa
      661 gcgaactgcc ggaagtctca attaattcgg atgacccggt ggccctgccg tttagctctg
      721 gtacgaccgg cctgccgaaa ggcgtggttc tgacgcacaa atcactgatc acctcggtcg
      781 cccagcaagt ggatggtgaa aacccgaatc tgcatctgac cccggatgac gtcgtgctgt
      841 gcgtgctgcc gctgttccac atttatagcc tgaactctgt tctgctgtgt agtctgcgtg
      901 caggtgcagc agtgctgctg atgcagaaat ttgaaattgg taccctgctg gaactgatcc
      961 aacgttaccg cgtgagcgtt gcagctgttg tcccgccgct ggttctggca ctggctaaaa
     1021 atccgatggt ggaatcgttt gatctgagtt ccatccgtgt ggttctgagc ggtgcagcac
     1081 cgctgggcaa agaactggaa gcagctctgc gttcccgcgt tccgcaggca gtcctgggcc
     1141 aaggttatgg catgacggaa gcaggcccgg tgctgtcaat gtgcctgggt ttcgctaaac
     1201 agccgtttcc gacgaaatca ggttcgtgtg gcaccgtcgt gcgtaacgcg gaactgaaag
     1261 ttgtggatcc ggaaaccggt tgctccctgg gccgtaatca gccgggtgaa atttgtatcc
     1321 gcggccagca aattatgaaa ggttatctga atgatccgga agcgacggcc tctaccattg
     1381 acgttgatgg ctggctgcat accggtgaca tcggctacgt ggatgacgat gaagaagtgt
     1441 tcattgttga tcgcgtcaaa gaactgatca aattcaaagg ttttcaggtt ccgccggcag
     1501 aactggaagc tctgctggtg tctcacccgt ccattgccga tgcggccgtg gttccgcaaa
     1561 aagacgatgt tgctggcgaa gtcccggtgg cgttcgtcgt gcgttctaac ggttttgaac
     1621 tgaccgaaga agcagtgaaa gaattcatca gtaaacaggt tgtcttttat aaacgcctgc
     1681 ataaagtgta ctttgttcac gcgattccga aaagcccgtc tggcaaaatc ctgcgtaaag
     1741 atctgcgcgc gaaactggcc gaaaaaaccc cggaaccgaa cggcggtggc ggtagtggcg
     1801 gtggcggttc cggcggtggc ggttcactgg tgggtgccct gatgcatgtt atgcagaaac
     1861 gtagccgtgc tatccactcc tcagatgaag gtgaagacca ggcaggtgac gaagatgaag
     1921 actaactcga gtctggtaaa gaaaccgctg ctgcgaaatt tgaacgccag cacatggact
     1981 cgtctactag tcgcagctta attaacctaa actgctgcca ccgctgagca ataactagca
     2041 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctaggatc gagatcgatc
     2101 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     2161 ctctagaaat aattttgttt aactttaaga aggagatata catatggcta cggtccaaga
     2221 aatccgcaac gctcaacgcg cagatggtcc ggcgacggtc ctggcaatcg gcacggcaac
     2281 cccggctcat agcgtgaacc aggcagatta tccggactat tactttcgta ttaccaaatc
     2341 tgaacacatg acggaactga aagaaaaatt caaacgtatg tgcgataaaa gtatgattaa
     2401 aaaacgctac atgtacctga ccgaagaaat cctgaaagaa aacccgaata tgtgtgccta
     2461 catggcaccg agcctggatg cgcgccagga cattgtggtt gtcgaagttc cgaaactggg
     2521 taaagaagcg gccaccaaag ccatcaaaga atggggccaa ccgaaatcaa aaattacgca
     2581 cctgatcttt tgcaccacgt cgggtgtgga tatgccgggt gcagactatc agctgaccaa
     2641 actgctgggt ctgcgtccga gcgttaaacg ctttatgatg taccagcaag gctgcttcgc
     2701 aggcggtacg gtcctgcgtc tggctaaaga tctggcggaa aacaataaag gtgctcgcgt
     2761 tctggtggtt tgtagtgaaa ttaccgctgt cacgtttcgt ggtccggcgg atacccatct
     2821 ggactccctg gttggccagg ccctgttcgg cgatggtgca gctgcggtta tcgtcggcgc
     2881 agatccggac acgagtgtgg aacgtccgct gtatcagctg gtttcaacct cgcaaacgat
     2941 tctgccggat tccgacggtg cgatcgatgg ccatctgcgc gaagtgggtc tgacctttca
     3001 cctgctgaaa gacgttccgg gcctgatttc aaaaaacatc gaaaaaagcc tgtctgaagc
     3061 ctttgcaccg gttggtattt cggattggag ctctattttc tggatcgcac atccgggcgg
     3121 tccggcaatc ctggaccagg tggaaagcaa actgggtctg aaagaagaaa aactgaaagc
     3181 tacccgtcaa gtcctgtctg aatacggcaa tatgagttcc gcgtgtgtgc tgttcattct
     3241 ggatgaaatg cgcaaaaaat ctgccgaaga agctaaagcg accacgggcg aaggtctgga
     3301 ttggggcgtg ctgtttggtt tcggtccggg tctgaccgtc gaaacggtcg tgctgcacag
     3361 tgtgccgatc aaagcgggcg gtggcggttc cggcggtggt ggtagtggtg gtggtggctc
     3421 tccgccgccg gccctgccgc cgaaacgtcg tcgctaactc gagtctggta aagaaaccgc
     3481 tgctgcgaaa tttgaacgcc agcacatgga ctcgtctact agtcgcagct taattaacct
     3541 aaactgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     3601 tgaggggttt tttgctagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac
     3661 tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa
     3721 gaaggagata tacatatgaa tccgtcgccg tctgttaccg aactgcaagt ggaaaatgtc
     3781 acctttacgc cgagtctgca accgccgggc tctaccaaat cgcattttct gggcggtgca
     3841 ggtgaacgtg gcctggaaat cgaaggcaaa tttgttaaat tcaccgctat tggtgtctat
     3901 ctggaagaaa acgccgtgcc gctgctggca ggcaaatgga aaggcaaaac cgccggtgaa
     3961 ctgacggaat ctgtcgaatt tttccgcgat gtggttaccg gcccgtttga aaaattcatg
     4021 aaagtgacca tgatcctgcc gctgacgggt gcgcagtatt cagaaaaagt tgctgaaaat
     4081 tgcatggcga tttggaaatt tttcggcatc tacaccgatg cagaagctaa agcgattgaa
     4141 aaatttacgg aagtgttcaa agacgaaatt tttccgccgg gcagctctat cctgttcacc
     4201 caaagttccg gttcgctgac gatttcattt tcgaaagatg gcagcatccc gaaagacggt
     4261 gtcgcggtga ttgaaaacaa tctgctgagc gaagccgttc tggaatctat gatcggtaaa
     4321 aacggcgtca gtccggcggc caaaaaatcc ctggccgaac gtctgtcagc actgctgaat
     4381 gttgcttccg acaaaatgaa aggcggtggc ggctcaggtg gcggtggctc tggtggcggt
     4441 ggttcaggcg tcaaagaaag tctggtgtga ctcgagtctg gtaaagaaac cgctgctgcg
     4501 aaatttgaac gccagcacat ggactcgtct actagtcgca gcttaattaa cctaaactgc
     4561 tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg
     4621 ttttttgcta gcgaaaggag gagtcgacta tatccggatt ggcgaatggg acgcgccctg
     4681 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4741 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4801 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4861 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4921 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4981 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5041 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     5101 taacaaaata ttaacgttta caatttctgg cggcacgatg gcatgagatt atcaaaaagg
     5161 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5221 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5281 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5341 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5401 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5461 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5521 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     5581 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     5641 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     5701 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     5761 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     5821 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     5881 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     5941 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6001 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6061 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatca
     6121 tgattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6181 gaaaaataaa caaataggtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6241 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6301 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6361 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6421 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6481 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6541 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6601 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6661 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6721 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6781 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6841 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6901 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6961 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7021 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     7081 gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata
     7141 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     7201 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     7261 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     7321 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     7381 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     7441 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     7501 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     7561 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     7621 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     7681 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     7741 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     7801 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     7861 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     7921 aacgacagga gcacgatcat gctagtcatg ccccgcgccc accggaagga gctgactggg
     7981 ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca
     8041 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     8101 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt
     8161 tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag
     8221 ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt
     8281 taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatgtc
     8341 cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc
     8401 gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg
     8461 aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg
     8521 agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc
     8581 cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt
     8641 accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa
     8701 taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg
     8761 atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca
     8821 ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc
     8881 gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc
     8941 aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta
     9001 attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc
     9061 ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta
     9121 taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc
     9181 cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat
     9241 gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg
     9301 caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca
     9361 ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat
     9421 cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca
     9481 cgatgcgtcc ggcgtagcct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
     9541 tcactata
//
