LOCUS       sequence-135146-        9305 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9305)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9305)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-At4CL-PhCHS-CmCHI (C5 mutant, pFlavo-opt)
FEATURES             Location/Qualifiers
     source          1..9305
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1770..1814
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1867..1914
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1948..1966
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1967..1991
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2006..2028
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3227..3271
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3324..3371
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    3424..3448
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3463..3485
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4240..4284
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4337..4384
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4428..4883
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4725..4746
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4998..5789,5790..5858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5621..5640
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5859..5951)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6032..6620
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6521..6540
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6774..6791
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6806..6948
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6874..6893)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7034..7056
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7050..7241)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7813..8895)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8857..8876
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8896..8973)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9179..9198
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9289..9305,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggcgcca caagaacaag cagtttctca ggtgatggag aaacagagca
      121 acaacaacaa cagtgacgtc attttccgat caaagttacc ggatatttac atcccgaacc
      181 acctatctct ccacgactac atcttccaaa acatctccga attcgccact aagccttgcc
      241 taatcaacgg accaaccggc cacgtgtaca cttactccga cgtccacgtc atctcccgcc
      301 aaatcgccgc caattttcac aaactcggcg ttaaccaaaa cgacgtcgtc atgctcctcc
      361 tcccaaactg tcccgaattc gtcctctctt tcctcgccgc ctccttccgc ggcgcaaccg
      421 ccaccgccgc aaaccctttc ttcactccgg cggagatagc taaacaagcc aaagcctcca
      481 acaccaaact cataatcacc gaagctcgtt acgtcgacaa aatcaaacca cttcaaaacg
      541 acgacggagt agtcatcgtc tgcatcgacg acaacgaatc cgtgccaatc cctgaaggct
      601 gcctccgctt caccgagttg actcagtcga caaccgaggc atcagaagtc atcgactcgg
      661 tggagatttc accggacgac gtggtggcac taccttactc ctctggcacg acgggattac
      721 caaaaggagt gatgctgact cacaagggac tagtcacgag cgttgctcag caagtcgacg
      781 gcgagaaccc gaatctttat ttccacagcg atgacgtcat actctgtgtt ttgcccatgt
      841 ttcatatcta cgctttgaac tcgatcatgt tgtgtggtct tagagttggt gcggcgattc
      901 tgataatgcc gaagtttgag atcaatctgc tattggagct gatccagagg tgtaaagtga
      961 cggtggctcc gatggttccg ccgattgtgt tggccattgc gaagtcttcg gagacggaga
     1021 agtatgattt gagctcgata agagtggtga aatctggtgc tgctcctctt ggtaaagaac
     1081 ttgaagatgc cgttaatgcc aagtttccta atgccaaact cggtcaggga tacggaatga
     1141 cggaagcagg tccagtgctc gcaatgtcgt taggttttgc aaaggaacct tttccggtta
     1201 agtcaggagc ttgtggtact gttgtaagaa atgctgagat gaaaatagtt gatccagaca
     1261 ccggagattc tctttcgagg aatcaacccg gtgagatttg tattcgtggt caccagatca
     1321 tgaaaggtta cctcaacaat ccggcagcta cagcagagac cattgataaa gacggttggc
     1381 ttcatactgg agatattgga ttgatcgatg acgatgacga gcttttcatc gttgatcgat
     1441 tgaaagaact tatcaagtat aaaggttttc aggtagctcc ggctgagcta gaggctttgc
     1501 tcatcggtca tcctgacatt actgatgttg ctgttgtcgc aatgaaagaa gaagcagctg
     1561 gtgaagttcc tgttgcattt gtggtgaaat cgaaggattc ggagttatca gaagatgatg
     1621 tgaagcaatt cgtgtcgaaa caggttgtgt tttacaagag aatcaacaaa gtgttcttca
     1681 ctgaatccat tcctaaagct ccatcaggga agatattgag gaaagatctg agggcaaaac
     1741 tagcaaatgg attgtgactc gagtctggta aagaaaccgc tgctgcgaaa tttgaacgcc
     1801 agcacatgga ctcgtctact agtcgcagct taattaacct aaactgctgc caccgctgag
     1861 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctagga
     1921 tcgagatcga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
     1981 ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggt
     2041 gacagtcgag gagtatcgta aggcacaacg tgctgaaggt ccagccactg tcatggccat
     2101 tggaacagcc acaccttcaa actgtgttga tcaaagcact taccctgatt tttattttcg
     2161 tatcactaac agtgagcaca agactgatct taaggagaaa tttaagcgca tgtgtgaaaa
     2221 atcaatgatt aagaaaaggt acatgcactt aacagaggaa atcttgaaag agaatcctag
     2281 tatgtgtgaa tacatggcac cttctcttga tgctaggcaa gacatagtgg tggttgaagt
     2341 gcccaaactt ggcaaagagg cagctcaaaa ggccatcaag gaatggggcc agcccaagtc
     2401 caaaattacc catttggtct tttgcacaac cagtggtgtg gacatgcctg ggtgtgacta
     2461 tcaactcact aagctacttg ggcttcgtcc atcggtcaag aggcttatga tgtaccaaca
     2521 aggttgcttt gctggtggca cggttcttcg gttagccaag gacttggctg aaaacaacaa
     2581 gggcgctcga gtccttgttg tttgttcaga aatcaccgcg gtcactttcc gtgggccaaa
     2641 tgatactcat ttggatagtt tagttggcca agcacttttt ggtgatgggg caggcgcgat
     2701 cattataggt tctgatccaa ttccaggggt cgaaaggcct ttgttcgagc tcgtttcagc
     2761 agcccaaact cttctcccag atagccatgg tgctattgat ggccatctcc gtgaagttgg
     2821 gcttacattc cacttactca aagatgttcc tgggctgatc tcaaaaaata ttgagaagag
     2881 ccttgaggaa gcattcaaac ctttgggcat ttctgattgg aactctctat tctggattgc
     2941 tcatccaggt gggcctgcaa ttttggacca agttgaaata aagttgggcc taaagcccga
     3001 gaaacttaag gctacaagga atgtgttaag taactatggt aacatgtcaa gtgcttgtgt
     3061 actgtttatt ttggatgaaa tgagaaaggc ctcagccaaa gaaggtttag gaactactgg
     3121 tgaagggctt gagtggggtg ttctttttgg atttgggcct gggctaacag ttgagactgt
     3181 tgtcctccac agtgttgcta cttaataggg taccctcgag tctggtaaag aaaccgctgc
     3241 tgcgaaattt gaacgccagc acatggactc gtctactagt cgcagcttaa ttaacctaaa
     3301 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     3361 ggggtttttt gctaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat
     3421 caaggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
     3481 ggagatatac atatgaatcc gtcgccgtct gttaccgaac tgcaagtgga aaatgtcacc
     3541 tttacgccga gtctgcaacc gccgggctct accaaatcgc attttctggg cggtgcaggt
     3601 gaacgtggcc tggaaatcga aggcaaattt gttaaattca ccgctattgg tgtctatctg
     3661 gaagaaaacg ccgtgccgct gctggcaggc aaatggaaag gcaaaaccgc cggtgaactg
     3721 acggaatctg tcgaattttt ccgcgatgtg gttaccggcc cgtttgaaaa attcatgaaa
     3781 gtgaccatga tcctgccgct gacgggtgcg cagtattcag aaaaagttgc tgaaaattgc
     3841 atggcgattt ggaaattttt cggcatctac accgatgcag aagctaaagc gattgaaaaa
     3901 tttacggaag tgttcaaaga cgaaattttt ccgccgggca gctctatcct gttcacccaa
     3961 agttccggtt cgctgacgat ttcattttcg aaagatggca gcatcccgaa agacggtgtc
     4021 gcggtgattg aaaacaatct gctgagcgaa gccgttctgg aatctatgat cggtaaaaac
     4081 ggcgtcagtc cggcggccaa aaaatccctg gccgaacgtc tgtcagcact gctgaatgtt
     4141 gcttccgaca aaatgaaagg cggtggcggc tcaggtggcg gtggctctgg tggcggtggt
     4201 tcaggcgtca aagaaagtct ggtgtgactc gagtctggta aagaaaccgc tgctgcgaaa
     4261 tttgaacgcc agcacatgga ctcgtctact agtcgcagct taattaacct aaactgctgc
     4321 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     4381 tttgctagcg aaaggaggag tcgactatat ccggattggc gaatgggacg cgccctgtag
     4441 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     4501 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     4561 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     4621 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     4681 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     4741 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     4801 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     4861 caaaatatta acgtttacaa tttctggcgg cacgatggca tgagattatc aaaaaggatc
     4921 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4981 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5041 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5101 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5161 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5221 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5281 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5341 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5401 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5461 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5521 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5581 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5641 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5701 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5761 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5821 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatcatga
     5881 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     5941 aaataaacaa ataggtcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6001 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6061 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6121 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     6181 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6241 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6301 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6361 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6421 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6481 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6541 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6601 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6661 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     6721 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     6781 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     6841 gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6901 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg
     6961 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
     7021 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
     7081 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc
     7141 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata
     7201 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg
     7261 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg
     7321 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta
     7381 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca
     7441 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg
     7501 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt
     7561 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt
     7621 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac
     7681 gacaggagca cgatcatgct agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     7741 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     7801 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     7861 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct
     7921 tttcaccagt gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
     7981 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
     8041 cggcgggata taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatgtccgc
     8101 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt
     8161 ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa
     8221 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt
     8281 gagatattta tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc
     8341 taacagcgcg atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc
     8401 gtcttcatgg gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa
     8461 cgccggaaca ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata
     8521 gttaatgatc agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc
     8581 ttcgacgccg cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg
     8641 agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac
     8701 gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt
     8761 cagctccgcc atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg
     8821 gttcaccacg cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa
     8881 cgttactggt ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat
     8941 accgcgaaag gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg
     9001 actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa
     9061 ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca
     9121 tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg
     9181 tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga
     9241 tgcgtccggc gtagcctagg atcgagatcg atctcgatcc cgcgaaatta atacgactca
     9301 ctata
//
