LOCUS       sequence-135148-         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Aurein1.2_+36GFP-Cys expression in E.Coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Li M, Tao Y, Shu Y, LaRochelle JR, Steinauer A, Thompson D, 
            Schepartz A, Chen ZY, Liu DR
  TITLE     Discovery and Characterization of a Peptide That Enhances Endosomal 
            Escape of Delivered Proteins in Vitro and in Vivo.
  JOURNAL   J Am Chem Soc. 2015 Nov 11;137(44):14084-93. doi: 
            10.1021/jacs.5b05694. Epub 2015 Oct 30.
  PUBMED    26465072
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b05694"; journalName: "J Am Chem Soc"; date: 
            "2015-11-11- 11"; volume: "137"; issue: "44"; pages: "14084-93"
COMMENT     Sequence Label: Aurein1.2_(pos36GFP)-Cys
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..59)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aatttcgcag cagcggtttc tttaccagac tcgagtcatt agtgatggtg gtggtgatgg
       61 gatcctccgc atccgctacc accggtcttg tagcgttcgt cgcgtccgtg cttaatgcct
      121 gcagcggtca cgaattctaa cagaaccatg tgatcgcgtt tttctttcgg atctttgcta
      181 agtttagaac gggtgctcag gtagtggttg cgaggtaaca gtacaggccc gcgaccaatc
      241 ggcgtgtttt gttgataatg atccgctaat tgaacgctgc cgtctttcac gttatggcga
      301 attttgaatt ttgccttgat accattcttg cgtttatcgg ccgtaatata caccttatga
      361 gaattaaagt tataacgcag tttatggcct aaaatgttgc ctttttcttt gaagtcacga
      421 cctttcagtt tgatacgatt tactaatgtg cggccttcaa atttcacttc cgcacgcgtt
      481 ttatatttgc catccttctt gaagctgatt gtacgctctt gaacatatcc tttaggcatg
      541 gctgatttga aaaaatcgtg acgtttcatg tgtttagggt aacgactgaa gcactgaacg
      601 ccataggtca gggtggtcac taaggtgggc cacggaacgg gtaatttgcc tgttgtgcaa
      661 ataaacttca gggtcaattt accacgggta gcgtcacctt tgcctttgcc gcgcacgcta
      721 aatttatgac cgttcacgtc gccctttaat tccactaaga tcggtacttt accacgaaac
      781 agacgttcac ctttgctaga accgccgctg ccaccgctac caccgctac
//
