LOCUS       sequence-135150-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PhyB-mCherry with prenylation sequence from Kras, for 
            red-light-induced recruitment of PIF to the plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL149
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacccaag
       61 cttggtacca tggtttccgg agtcgggggt agtggcggtg gccgtggcgg tggccgtggc
      121 ggagaagaag aaccgtcgtc aagtcacact cctaataacc gaagaggagg agaacaagct
      181 caatcgtcgg gaacgaaatc tctcagacca agaagcaaca ctgaatcaat gagcaaagca
      241 attcaacagt acaccgtcga cgcaagactc cacgccgttt tcgaacaatc cggcgaatca
      301 gggaaatcat tcgactactc acaatcactc aaaacgacga cgtacggttc ctctgtacct
      361 gagcaacaga tcacagctta tctctctcga atccagcgag gtggttacat tcagcctttc
      421 ggatgtatga tcgccgtcga tgaatccagt ttccggatca tcggttacag tgaaaacgcc
      481 agagaaatgt tagggattat gcctcaatct gttcctactc ttgagaaacc tgagattcta
      541 gctatgggaa ctgatgtgag atctttgttc acttcttcga gctcgattct actcgagcgt
      601 gctttcgttg ctcgagagat taccttgtta aatccggttt ggatccattc caagaatact
      661 ggtaaaccgt tttacgccat tcttcatagg attgatgttg gtgttgttat tgatttagag
      721 ccagctagaa ctgaagatcc tgcgctttct attgctggtg ctgttcaatc gcaga
//
