LOCUS       sequence-135158-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Tsun ZY, Bar-Peled L, Chantranupong L, Zoncu R, Wang T, Kim C, 
            Spooner E, Sabatini DM
  TITLE     The folliculin tumor suppressor is a GAP for the RagC/D GTPases that
            signal amino acid levels to mTORC1.
  JOURNAL   Mol Cell. 2013 Nov 21;52(4):495-505. doi: 
            10.1016/j.molcel.2013.09.016. Epub 2013 Oct 3.
  PUBMED    24095279
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2013.09.016"; journalName: "Mol Cell"; date: 
            "2013-11-21- 21"; volume: "52"; issue: "4"; pages: "495-505"
COMMENT     Sequence Label: pRK5-FLAG-FLCN (C terminus)
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      826..835
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(877..896)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tccactccca ggtccaactg cacctcggtt ctatcgattg aattcaccat ggaaatggac
       61 tacaaggatg acgatgacaa gggtggtggt ggtggtgcgt cgacgatgcg gaagctgcca
      121 gtcttcaagt ccctccggca catgaggcag gtcctgggtg ccccttcttt ccgcatgctg
      181 gcctggcacg ttctcatggg gaaccaggtg atctggaaaa gcagagacgt ggacctcgtc
      241 cagtcagctt ttgaagtact tcggaccatg cttcccgtgg gctgcgtccg catcatccca
      301 tacagcagcc agtacgagga ggcctatcgg tgcaacttcc tggggctcag cccgcacgtg
      361 cagatccccc cccacgtgct ctcctcagag tttgctgtca tcgtggaggt ccacgcagcc
      421 gcacgttcca ccctccaccc tgtggggtgt gaggatgacc agtctctcag caagtacgag
      481 tttgtggtga ccagtgggag ccctgtagct gcagaccgag tgggccccac catcctgaat
      541 aagattgaag cggctctgac caaccagaac ctgtctgtgg atgtggtgga ccagtgcctc
      601 gtctgcctca aggaggagtg gatgaacaaa gtgaaggtgc tttttaagtt caccaaggtg
      661 gacagtcgcc ccaaagagga cacacagaag ctgctgagca tcctgggtgc gtccgaggag
      721 gacaatgtca agctgctgaa gttctggatg actggcctga gcaagaccta caagtcacac
      781 ctcatgtcct gagcggccgc taagtaagta aggatcccca gcttggccgc catggcccaa
      841 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      901 ataaagcatt tt
//
