LOCUS       sequence-135169-         988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses S. pyogenes CRISPR chimeric RNA element with customizable 
            sgRNA from U6 promoter and Crimson reporter from EF-1a promoter. 
            Lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 988)
  AUTHORS   Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen 
            DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, 
            Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE
  TITLE     BCL11A enhancer dissection by Cas9-mediated in situ saturating 
            mutagenesis.
  JOURNAL   Nature. 2015 Sep 16. doi: 10.1038/nature15521.
  PUBMED    26375006
REFERENCE   2  (bases 1 to 988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 16. doi: 10.1038/nature15521."
COMMENT     Sequence Label: lentiGuide-Crimson
FEATURES             Location/Qualifiers
     source          1..988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        6..254
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6..26
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     177..196
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 ccagagaggg cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta
       61 gagagataat tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac
      121 gtagaaagta ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact
      181 atcatatgct taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
      241 aaggacgaaa caccggagac ggttgtaaat gagcacacaa aatacacatg ctaaaatatt
      301 atattctatg acctttataa aatcaaccaa aatcttcttt ttaataactt tagtatcaat
      361 aattagaatt tttatgttcc tttttgcaaa cttttaataa aaatgagcaa aataaaaaaa
      421 cgctagtttt agtaactcgc gttgttttct tcacctttaa taatagctac tccaccactt
      481 gttcctaagc ggtcagctcc tgcttcaatc attttttgag catcttcaaa tgttctaact
      541 ccaccagctg ctttaactaa agcattgtct ttaacaactg acttcattag tttaacatct
      601 tcaaatgttg cacctgattt tgaaaatcct gttgatgttt taacaaattc taatccagct
      661 tcaacagcta tttcacaagc tttcatgatt tcttcttttg ttaataaaca attttccata
      721 atacatttaa caacatgtga tccagctgct ttttttacag ctttcatgtc ttctaaaact
      781 aattcataat ttttgtcttt taatgcacca atatttaata ccatatcaat ttctgttgca
      841 ccatctttaa ttgcttcaga aacttcgaat gcttttgtag ctgttgtgca tgcacctaga
      901 ggaaaaccta caacatttgt tattcctaca tttgtgcctt ttaataattc tttacaatag
      961 cttgttcaat atgaattaac acaaactg
//
