LOCUS       sequence-135175-         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses human codon-optimized S. pyogenes Cas9 protein and Venus 
            reporter from EFS promoter. Lentiviral backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Canver MC, Smith EC, Sher F, Pinello L, Sanjana NE, Shalem O, Chen 
            DD, Schupp PG, Vinjamur DS, Garcia SP, Luc S, Kurita R, Nakamura Y, 
            Fujiwara Y, Maeda T, Yuan GC, Zhang F, Orkin SH, Bauer DE
  TITLE     BCL11A enhancer dissection by Cas9-mediated in situ saturating 
            mutagenesis.
  JOURNAL   Nature. 2015 Sep 16. doi: 10.1038/nature15521.
  PUBMED    26375006
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Sep 16. doi: 10.1038/nature15521."
COMMENT     Sequence Label: lentiCas9-Venus
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
       61 ttcgcaacgg gtttgccgcc agaacacagg accggttcta gagcgctgcc accatggaca
      121 agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg
      181 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
      241 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
      301 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
      361 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
      421 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
      481 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
      541 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
      601 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
      661 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg
      721 ccagcggcgt ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg
      781 aaaatctgat cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg
      841 ccctgagcct gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca
      901 aactgcagct gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg
      961 gcgaccagta cgccgacctg tttctggccg c
//
