LOCUS       sequence-135191-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral stable expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Wang S, Tsun ZY, Wolfson RL, Shen K, Wyant GA, Plovanich ME, Yuan 
            ED, Jones TD, Chantranupong L, Comb W, Wang T, Bar-Peled L, Zoncu R,
            Straub C, Kim C, Park J, Sabatini BL, Sabatini DM
  TITLE     Metabolism. Lysosomal amino acid transporter SLC38A9 signals 
            arginine sufficiency to mTORC1.
  JOURNAL   Science. 2015 Jan 9;347(6218):188-94. doi: 10.1126/science.1257132. 
            Epub 2015 Jan 7.
  PUBMED    25567906
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1257132"; journalName: "Science"; date: "2015-01-9-
            9"; volume: "347"; issue: "6218"; pages: "188-94"
COMMENT     Sequence Label: pLJM60-FLAG-SLC38A9.1
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        223..241
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     223..240
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     254..273
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             332..355
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aaccgtcaga tcgcctggag acgccatcca cgctgttttt gacctccata gaagacaccg
       61 ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc cccgtgccaa
      121 gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt cgttagaacg
      181 cggctacaat taatacataa ccttatgtat catacacata cgatttaggt gacactatag
      241 aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca actgcacctc
      301 ggttctatcg attgaattca ccatggaaat ggactacaag gatgacgatg acaagggtgg
      361 tggtggtggt gcgtcgacaa tggctaacat gaacagtgac tcaagacatc tgggaacaag
      421 cgaggtggac cacgagcggg accctggccc tatgaacatt cagtttgagc cctccgacct
      481 gcggtctaag agacccttct gcatcgaacc taccaacatt gtgaacgtca atcacgtgat
      541 ccagagggtc agtgatcatg catcagccat gaataagcga attcactact attcccggct
      601 gaccacaccc gctgacaaag cactgatcgc ccctgatcat gtggtcccag cacccgagga
      661 atgttacgtg tatagccctc tgggatccgc ctacaagctg cagagctaca ccgaggggta
      721 tggaaaaaac acatccctgg tgactatttt tatgatctgg aataccatga tggggacaag
      781 tattctgtca atcccatggg gaatcaagca ggccgggttc actaccggaa tgtgcgtgat
      841 cattctgatg ggcctgctga ccctgtactg ctgttatagg gtggtcaaat ctcgcacaat
//
