LOCUS       sequence-135200-         495 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Cdt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Tsanov N, Kermi C, Coulombe P, Van der Laan S, Hodroj D, Maiorano D
  TITLE     PIP degron proteins, substrates of CRL4Cdt2, and not PIP boxes, 
            interfere with DNA polymerase eta and kappa focus formation on UV 
            damage.
  JOURNAL   Nucleic Acids Res. 2014 Apr;42(6):3692-706. doi: 
            10.1093/nar/gkt1400. Epub 2014 Jan 14.
  PUBMED    24423875
REFERENCE   2  (bases 1 to 495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkt1400"; journalName: "Nucleic Acids Res"; date: 
            "2014-04"; volume: "42"; issue: "6"; pages: "3692-706"
COMMENT     Sequence Label: pcDNA3-Cdt1 human
FEATURES             Location/Qualifiers
     source          1..495
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             159..431
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
ORIGIN
        1 ctagaagaac ccactgcctt actggcttat cgaaattaat acgactcact atagggagac
       61 ccaagcttac catggagcag cgccgcgtca ccgacttctt cgcgcgccgc cgcccgggac
      121 ccccccgcat cgcgccgccc aagctggcct gccgcacccc cagccccgcc aggcccgcac
      181 tccgcgcccc ggcctccgct accagtggca gccgcaagcg cgcccgcccg cccgccgccc
      241 ccggacgcga ccaggccagg ccaccggccc gcaggagact gcggctgtcg gtggacgagg
      301 tttccagccc cagtaccccc gaggccccag acatcccagc ctgcccttct ccgggccaga
      361 agataaagaa atccaccccg gcagcaggtc agccgcccca cctgacatcc gcgcaggacc
      421 aggacaccat ctctgagctt gcgtcatgcc tgcaacgggc ccgggagctg ggggcaagag
      481 tccgggcgct gaagg
//
