LOCUS       sequence-135209-       11153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses p53 tagged with mKate2 and split C-term mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Gaglia G, Lahav G
  TITLE     Constant rate of p53 tetramerization in response to DNA damage 
            controls the p53 response.
  JOURNAL   Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168.
  PUBMED    25344068
REFERENCE   2  (bases 1 to 11153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-10-24"; volume: "10:753. doi"; pages: " 
            10.15252/msb.20145168"
COMMENT     Sequence Label: pRRLHygro-pEF1a-p53ash-mKate2-splitmVenusC
FEATURES             Location/Qualifiers
     source          1..11153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2449..2629
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2676..2801
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3294..3527
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3712..3756
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4023..4140
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4180..4200
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4201..5379
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4432..5370
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5327..5347
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5385..5409
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5412..6590
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     protein_bind    complement(6592..6616)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6618..7313
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     primer_bind     7523..7544
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7591..7611)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7649..7669)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7654..7983
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7834..7969
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8057..9082
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8057..9082
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    9235..9823
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9288..9308)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9706..9717)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9898..10131
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10203..10324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10240..10259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10294..10313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10343..10478
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10510..10529)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10511..10529)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10539..10555)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10548..10570)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10696..11151
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10783..10802)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10993..11014
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaatttaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     2221 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2281 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     2341 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2401 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgg gtctctctgg
     2461 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2521 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2581 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     2641 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     2701 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2761 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2821 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2881 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2941 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     3001 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     3061 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     3121 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     3181 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     3241 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     3301 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     3361 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     3421 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     3481 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     3541 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     3601 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     3661 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     3721 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     3781 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     3841 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     3901 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     3961 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     4021 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     4081 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     4141 atcgataagc ttgatatcac catgattacg ccaagctatc aactttgtat agaaaagttg
     4201 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     4261 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     4321 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     4381 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     4441 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     4501 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     4561 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     4621 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     4681 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     4741 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     4801 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     4861 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     4921 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     4981 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     5041 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     5101 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     5161 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     5221 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     5281 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     5341 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattcaagt ttgtacaaaa
     5401 aagcaggctt catggaggag ccgcagtcag atcctagcgt cgagccccct ctgagtcagg
     5461 aaacattttc agacctatgg aaactacttc ctgaaaacaa cgttctgtcc cccttgccgt
     5521 cccaagcaat ggatgatttg atgctgtccc cggacgatat tgaacaatgg ttcactgaag
     5581 acccaggtcc agatgaagct cccagaatgc cagaggctgc tccccgcgtg gcccctgcac
     5641 cagcagctcc tacaccggcg gcccctgcac cagccccctc ctggcccctg tcatcttctg
     5701 tcccttccca gaaaacctac cagggcagct acggtttccg tctgggcttc ttgcattctg
     5761 ggacagccaa gtctgtgact tgcacgtact cccctgccct caacaagatg ttttgccaac
     5821 tggccaagac ctgccctgtg cagctgtggg ttgattccac acccccgccc ggcacccgcg
     5881 tccgcgccat ggccatctac aagcagtcac agcacatgac ggaggttgtg aggcgctgcc
     5941 cccaccatga gcgctgctca gatagcgatg gtctggcccc tcctcagcat cttatccgag
     6001 tggagggaaa tttgcgtgtg gagtatttgg atgacagaaa cacttttcga catagtgtgg
     6061 tggtgcccta tgagccgcct gaggttggct ctgactgtac caccatccac tacaactaca
     6121 tgtgtaacag ttcctgcatg ggcggcatga accggaggcc catcctcacc atcatcacac
     6181 tggaggatag ctctggaaac ctactgggac ggaacagctt tgaggtgcgt gtttgtgcct
     6241 gtcctgggag agaccggcgc acagaggaag agaatctccg caagaaaggg gagcctcacc
     6301 acgagctgcc cccagggagc actaagcgag cactgcccaa caacaccagc tcctctcccc
     6361 agccaaagaa gaaaccactg gatggagaat atttcaccct tcagatccgt gggcgtgagc
     6421 gcttcgagat gttccgagag ctgaatgagg ccttggaact caaggatgcc caggctggga
     6481 aggagccagg ggggagcagg gctcactcca gccacctgaa gtccaaaaag ggtcagtcta
     6541 cctcccgcca taaaaaactc atgttcaaga cagaagggcc tgactcagac gacccagctt
     6601 tcttgtacaa agtggccatg gtgtctgagc tgattaagga gaacatgcac atgaagctgt
     6661 acatggaggg caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc
     6721 cctacgaggg cacccagacc atgagaatca aggccgtcga gggcggccct ctccccttcg
     6781 ccttcgacat cctggctacc agcttcatgt acggcagcaa aaccttcatc aaccacaccc
     6841 agggcatccc cgacttcttt aagcagtcct tccctgaggg cttcacatgg gagagagtca
     6901 ccacatacga agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct
     6961 gcctcatcta caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc
     7021 agaagaaaac actcggctgg gaggcctcca ccgagaccct gtaccccgct gacggcggcc
     7081 tggaaggcag agccgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact
     7141 tgaagaccac atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact
     7201 atgtggacag aagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc
     7261 acgaggtggc tgtggccaga tactgcgacc tccctagcaa actggggcac agaggaggag
     7321 gtggaggtgg aggtggggga gccaagaacg gcatcaaggc caacttcaag atccgccaca
     7381 acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     7441 acggccccgt gctgctgccc gacaaccact acctgagcta ccagtccaag ctgagcaaag
     7501 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     7561 ctctcggcat ggacgagctg tacaagtaat caactttatt atacatagtt gataattcac
     7621 tggccgtcga gatccggact agtcctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7741 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7801 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7921 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7981 aaaaagcttg ccagctgggg cgccctctgg taaggttggg aagccctgca gatctctcgt
     8041 ccgggggcaa tgagatatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct
     8101 gatcgaaaag ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg
     8161 tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga
     8221 tggtttctac aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc
     8281 ggaagtgctt gacattgggg aatttagcga gagcctgacc tattgcatct cccgccgtgc
     8341 acagggtgtc acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt
     8401 cgcggaggcc atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc
     8461 attcggaccg caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc
     8521 tgatccccat gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc
     8581 gcaggctctc gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt
     8641 gcacgcggat ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat
     8701 tgactggagc gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg
     8761 gaggccgtgg ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga
     8821 gcttgcagga tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta
     8881 tcagagcttg gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc
     8941 aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
     9001 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
     9061 tcgtccgagg gcaaaggaat agagtagatg ccgaccgaac aagagctgat ttcgagaacg
     9121 cctcagccag caactcgcgc gagcctagca aggcatcgat aagcttgata tcgaattcct
     9181 gcagcccggg ggatccacta gttaacgcgt cgaccagcta gctggccagt cgacaatcaa
     9241 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9301 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9361 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9421 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9481 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9541 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     9601 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     9661 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9721 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     9781 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac
     9841 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     9901 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     9961 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
    10021 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
    10081 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
    10141 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
    10201 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
    10261 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
    10321 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc
    10381 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
    10441 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgcg tcgagacgta
    10501 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    10561 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    10621 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
    10681 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
    10741 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
    10801 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
    10861 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    10921 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    10981 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    11041 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    11101 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tcc
//
