LOCUS       sequence-135219-        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses FLAG tagged human BRD4 ET domain (608-699 aa) with F656W 
            mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: pcDNA-ET-F656W
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(108..437)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      122..257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(176..195)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     422..442
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(451..879)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(561..582)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     773..792
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct
       61 gtctcttgat cagatccgaa aatggatata caagctcccg ggagcttttt gcaaaagcct
      121 aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggcaga ggcggcctcg
      181 gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc
      241 ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga
      301 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca cctggttgct
      361 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca
      421 caccctaact gacacacatt ccacagaatt aattcgcgtt aaatttttgt taaatcagct
      481 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
      541 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
      601 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
      661 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
      721 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
      781 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
      841 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtg gggatacccc ctagagcccc
      901 agctggttct ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat
      961 tgtcttccca atcctccccc ttgctgtcct gccccacccc acccc
//
