LOCUS       sequence-135228-       11049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mutated p53-L344P tagged with mKate2 and split N-term 
            mVenus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11049)
  AUTHORS   Gaglia G, Lahav G
  TITLE     Constant rate of p53 tetramerization in response to DNA damage 
            controls the p53 response.
  JOURNAL   Mol Syst Biol. 2014 Oct 24;10:753. doi: 10.15252/msb.20145168.
  PUBMED    25344068
REFERENCE   2  (bases 1 to 11049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-10-24"; volume: "10:753. doi"; pages: " 
            10.15252/msb.20145168"
COMMENT     Sequence Label: pRRLNeo-pEF1a-p53ashL344P-mKate2-splitmVenusN
FEATURES             Location/Qualifiers
     source          1..11049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(132..200,201..992)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(350..369)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1652..1671
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1905..1922
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2075..2080,2081..2098,2099..2105)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2118..2140
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2137..2153
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     LTR             2449..2629
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2676..2801
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3298..3531
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3716..3760
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4053..4170
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4210..4230
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        4231..5409
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4462..5400
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5357..5377
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    5415..5439
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5442..6620
                     /codon_start=1
                     /gene="Homo sapiens TP53"
                     /product="human tumor suppressor protein that is often 
                     mutated in cancers"
                     /label=p53
                     /note="responds to cellular stresses to regulate the 
                     expression of target genes"
                     /translation="MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLML
                     SPDDIEQWFTEDPGPDEAPRMPEAAPRVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQ
                     GSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIY
                     KQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEP
                     PEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRD
                     RRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEM
                     FREPNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD"
     protein_bind    complement(6622..6646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6648..7343
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             join(7374..7376,7377..7379,7380..7838)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITA"
     primer_bind     complement(7419..7440)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7680..7699)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(7852..7872)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        7904..8260
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        7904..8094
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(7926..7946)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8111..8246
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8295..9089
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8295..9089
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8349..8368)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8959..8978
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     misc_feature    9131..9719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9184..9204)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9602..9613)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9794..10027
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10099..10220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10136..10155)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10190..10209
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10239..10374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10406..10425)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10407..10425)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10435..10451)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10444..10466)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10592..11047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10679..10698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10889..10910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaatttaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     2221 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2281 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
     2341 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2401 cgcattgcag agatattgta tttaagtgcc tagctcgata caataaacgg gtctctctgg
     2461 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2521 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2581 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     2641 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     2701 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2761 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     2821 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     2881 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     2941 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     3001 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     3061 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     3121 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     3181 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     3241 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     3301 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     3361 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     3421 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     3481 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     3541 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     3601 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     3661 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     3721 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     3781 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     3841 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     3901 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     3961 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     4021 ccgaattcac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     4081 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     4141 caaaaacaaa ttacaaaaat tcaaaatttt atcgataagc ttgatatcac catgattacg
     4201 ccaagctatc aactttgtat agaaaagttg ggctccggtg cccgtcagtg ggcagagcgc
     4261 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
     4321 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     4381 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     4441 gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt
     4501 acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt
     4561 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     4621 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     4681 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     4741 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     4801 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     4861 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     4921 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     4981 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     5041 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     5101 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     5161 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     5221 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     5281 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     5341 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     5401 gtgtcgtgag gaattcaagt ttgtacaaaa aagcaggctt catggaggag ccgcagtcag
     5461 atcctagcgt cgagccccct ctgagtcagg aaacattttc agacctatgg aaactacttc
     5521 ctgaaaacaa cgttctgtcc cccttgccgt cccaagcaat ggatgatttg atgctgtccc
     5581 cggacgatat tgaacaatgg ttcactgaag acccaggtcc agatgaagct cccagaatgc
     5641 cagaggctgc tccccgcgtg gcccctgcac cagcagctcc tacaccggcg gcccctgcac
     5701 cagccccctc ctggcccctg tcatcttctg tcccttccca gaaaacctac cagggcagct
     5761 acggtttccg tctgggcttc ttgcattctg ggacagccaa gtctgtgact tgcacgtact
     5821 cccctgccct caacaagatg ttttgccaac tggccaagac ctgccctgtg cagctgtggg
     5881 ttgattccac acccccgccc ggcacccgcg tccgcgccat ggccatctac aagcagtcac
     5941 agcacatgac ggaggttgtg aggcgctgcc cccaccatga gcgctgctca gatagcgatg
     6001 gtctggcccc tcctcagcat cttatccgag tggagggaaa tttgcgtgtg gagtatttgg
     6061 atgacagaaa cacttttcga catagtgtgg tggtgcccta tgagccgcct gaggttggct
     6121 ctgactgtac caccatccac tacaactaca tgtgtaacag ttcctgcatg ggcggcatga
     6181 accggaggcc catcctcacc atcatcacac tggaggatag ctctggaaac ctactgggac
     6241 ggaacagctt tgaggtgcgt gtttgtgcct gtcctgggag agaccggcgc acagaggaag
     6301 agaatctccg caagaaaggg gagcctcacc acgagctgcc cccagggagc actaagcgag
     6361 cactgcccaa caacaccagc tcctctcccc agccaaagaa gaaaccactg gatggagaat
     6421 atttcaccct tcagatccgt gggcgtgagc gcttcgagat gttccgagag ccgaatgagg
     6481 ccttggaact caaggatgcc caggctggga aggagccagg ggggagcagg gctcactcca
     6541 gccacctgaa gtccaaaaag ggtcagtcta cctcccgcca taaaaaactc atgttcaaga
     6601 cagaagggcc tgactcagac gacccagctt tcttgtacaa agtgggaatg gtgtctgagc
     6661 tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact
     6721 tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca
     6781 aggccgtcga gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt
     6841 acggcagcaa aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct
     6901 tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg
     6961 ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg
     7021 tgaacttccc atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca
     7081 ccgagaccct gtaccccgct gacggcggcc tggaaggcag agccgacatg gccctgaagc
     7141 tcgtgggcgg gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg
     7201 ctaagaacct caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg
     7261 aggccgacaa agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc
     7321 tccctagcaa actggggcac agaggaggag gtggaggtgg aggtaaagtg ggaatggtga
     7381 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     7441 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     7501 tgaccctgaa gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     7561 ccaccctggg ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg
     7621 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     7681 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     7741 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     7801 agtacaacta caacagccac aacgtctata tcaccgccga caagcagtaa tcaactttat
     7861 tatacatagt tgataattca ctggccgtcg agatccggac tagtgaggcg gaaagaacca
     7921 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     7981 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     8041 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     8101 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     8161 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
     8221 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa gatcgatcaa gagacaggat
     8281 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     8341 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     8401 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     8461 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     8521 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     8581 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     8641 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     8701 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     8761 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     8821 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     8881 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     8941 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     9001 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     9061 tctatcgcct tcttgacgag ttcttctgag cgactagtta acgcgtcgac cagctagctg
     9121 gccagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     9181 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     9241 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     9301 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     9361 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     9421 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     9481 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     9541 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     9601 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     9661 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc
     9721 tgagatcctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     9781 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     9841 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9901 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9961 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10021 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
    10081 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10201 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
    10261 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
    10321 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
    10381 tttgcgtcga gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
    10441 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
    10501 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
    10561 ccaacagttg cgcagcctga atggcgaatg gacgcgccct gtagcggcgc attaagcgcg
    10621 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    10681 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    10741 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    10801 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    10861 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    10921 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    10981 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
    11041 acaatttcc
//
