LOCUS       sequence-135229-         954 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral stable expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 954)
  AUTHORS   Wang S, Tsun ZY, Wolfson RL, Shen K, Wyant GA, Plovanich ME, Yuan 
            ED, Jones TD, Chantranupong L, Comb W, Wang T, Bar-Peled L, Zoncu R,
            Straub C, Kim C, Park J, Sabatini BL, Sabatini DM
  TITLE     Metabolism. Lysosomal amino acid transporter SLC38A9 signals 
            arginine sufficiency to mTORC1.
  JOURNAL   Science. 2015 Jan 9;347(6218):188-94. doi: 10.1126/science.1257132. 
            Epub 2015 Jan 7.
  PUBMED    25567906
REFERENCE   2  (bases 1 to 954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1257132"; journalName: "Science"; date: "2015-01-9-
            9"; volume: "347"; issue: "6218"; pages: "188-94"
COMMENT     Sequence Label: pLJM60-FLAG-SLC38A9.1 I68A
FEATURES             Location/Qualifiers
     source          1..954
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        222..240
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     222..239
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     253..272
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             331..354
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg
       61 gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc ccgtgccaag
      121 agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc gttagaacgc
      181 ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg acactataga
      241 ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa ctgcacctcg
      301 gttctatcga ttgaattcac catggaaatg gactacaagg atgacgatga caagggtggt
      361 ggtggtggtg cgtcgacaat ggctaacatg aacagtgact caagacatct gggaacaagc
      421 gaggtggacc acgagcggga ccctggccct atgaacattc agtttgagcc ctccgacctg
      481 cggtctaaga gacccttctg catcgaacct accaacattg tgaacgtcaa tcacgtgatc
      541 cagagggtca gtgatcatgc atcagccatg aataagcgag cccactacta ttcccggctg
      601 accacacccg ctgacaaagc actgatcgcc cctgatcatg tggtcccagc acccgaggaa
      661 tgttacgtgt atagccctct gggatccgcc tacaagctgc agagctacac cgaggggtat
      721 ggaaaaaaca catccctggt gactattttt atgatctgga ataccatgat ggggacaagt
      781 attctgtcaa tcccatgggg aatcaagcag gccgggttca ctaccggaat gtgcgtgatc
      841 attctgatgg gcctgctgac cctgtactgc tgttataggg tggtcaaatc tcgcacaatg
      901 atgttttcac tggacacaac tagctgggag taccccgatg tgtgtcggca ctat
//
