LOCUS       sequence-135239-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral stable expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Wang S, Tsun ZY, Wolfson RL, Shen K, Wyant GA, Plovanich ME, Yuan 
            ED, Jones TD, Chantranupong L, Comb W, Wang T, Bar-Peled L, Zoncu R,
            Straub C, Kim C, Park J, Sabatini BL, Sabatini DM
  TITLE     Metabolism. Lysosomal amino acid transporter SLC38A9 signals 
            arginine sufficiency to mTORC1.
  JOURNAL   Science. 2015 Jan 9;347(6218):188-94. doi: 10.1126/science.1257132. 
            Epub 2015 Jan 7.
  PUBMED    25567906
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1257132"; journalName: "Science"; date: "2015-01-9-
            9"; volume: "347"; issue: "6218"; pages: "188-94"
COMMENT     Sequence Label: pLJM60-FLAG-SLC38A9.1 (delta110)
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        230..248
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     230..247
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     261..280
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             339..362
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac ctccatagaa
       61 gacaccggga ccgatccagc ctccgcggcc gggaacggtg cattggaacg cggattcccc
      121 gtgccaagag tgacgtaagt accgcctata gagtctatag gcccaccccc ttggcttcgt
      181 tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga tttaggtgac
      241 actatagaat aacatccact ttgcctttct ctccacaggt gtccactccc aggtccaact
      301 gcacctcggt tctatcgatt gaattcacca tggaaatgga ctacaaggat gacgatgaca
      361 agggtggtgg tggtggtgcg tcgacaaccg aggggtatgg aaaaaacaca tccctggtga
      421 ctatttttat gatctggaat accatgatgg ggacaagtat tctgtcaatc ccatggggaa
      481 tcaagcaggc cgggttcact accggaatgt gcgtgatcat tctgatgggc ctgctgaccc
      541 tgtactgctg ttatagggtg gtcaaatctc gcacaatgat gttttcactg gacacaacta
      601 gctgggagta ccccgatgtg tgtcggcact attttggaag cttcggccag tggagctccc
      661 tgctgttcag cctggtgtcc ctgattggcg ctatgatcgt gtactgggtc ctgatgagca
      721 acttcctgtt taataccggg aagttcatct ttaacttcat ccaccatatc aacgacaccg
      781 atacaatcct gtctacaaac aatagtaacc ctgtgatttg cccatctgcc ggaagtggcg
      841 ggcatccaga caactctagt atgatctttt acgctaatga tactggcgca cagcagttcg
      901 agaagtggtg ggacaaaagc agaaccgtgc ccttttatct ggtcggcctg ctg
//
