LOCUS       sequence-135247-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral stable expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Wang S, Tsun ZY, Wolfson RL, Shen K, Wyant GA, Plovanich ME, Yuan 
            ED, Jones TD, Chantranupong L, Comb W, Wang T, Bar-Peled L, Zoncu R,
            Straub C, Kim C, Park J, Sabatini BL, Sabatini DM
  TITLE     Metabolism. Lysosomal amino acid transporter SLC38A9 signals 
            arginine sufficiency to mTORC1.
  JOURNAL   Science. 2015 Jan 9;347(6218):188-94. doi: 10.1126/science.1257132. 
            Epub 2015 Jan 7.
  PUBMED    25567906
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1257132"; journalName: "Science"; date: "2015-01-9-
            9"; volume: "347"; issue: "6218"; pages: "188-94"
COMMENT     Sequence Label: pLJM60-FLAG-SLC38A9.1 H60A
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        225..243
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     225..242
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     256..275
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             334..357
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
       61 cgggaccgat ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc
      121 aagagtgacg taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa
      181 cgcggctaca attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat
      241 agaataacat ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc
      301 tcggttctat cgattgaatt caccatggaa atggactaca aggatgacga tgacaagggt
      361 ggtggtggtg gtgcgtcgac aatggctaac atgaacagtg actcaagaca tctgggaaca
      421 agcgaggtgg accacgagcg ggaccctggc cctatgaaca ttcagtttga gccctccgac
      481 ctgcggtcta agagaccctt ctgcatcgaa cctaccaaca ttgtgaacgt caatcacgtg
      541 atccagaggg tcagtgatgc cgcatcagcc atgaataagc gaattcacta ctattcccgg
      601 ctgaccacac ccgctgacaa agcactgatc gcccctgatc atgtggtccc agcacccgag
      661 gaatgttacg tgtatagccc tctgggatcc gcctacaagc tgcagagcta caccgagggg
      721 tatggaaaaa acacatccct ggtgactatt tttatgatct ggaataccat gatgg
//
