LOCUS       sequence-135252-         985 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral stable expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 985)
  AUTHORS   Wang S, Tsun ZY, Wolfson RL, Shen K, Wyant GA, Plovanich ME, Yuan 
            ED, Jones TD, Chantranupong L, Comb W, Wang T, Bar-Peled L, Zoncu R,
            Straub C, Kim C, Park J, Sabatini BL, Sabatini DM
  TITLE     Metabolism. Lysosomal amino acid transporter SLC38A9 signals 
            arginine sufficiency to mTORC1.
  JOURNAL   Science. 2015 Jan 9;347(6218):188-94. doi: 10.1126/science.1257132. 
            Epub 2015 Jan 7.
  PUBMED    25567906
REFERENCE   2  (bases 1 to 985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1257132"; journalName: "Science"; date: "2015-01-9-
            9"; volume: "347"; issue: "6218"; pages: "188-94"
COMMENT     Sequence Label: pLJM60-FLAG-SLC38A9.1 Y71A
FEATURES             Location/Qualifiers
     source          1..985
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        233..251
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     233..250
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     264..283
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             342..365
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
       61 gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga acgcggattc
      121 cccgtgccaa gagtgacgta agtaccgcct atagagtcta taggcccacc cccttggctt
      181 cgttagaacg cggctacaat taatacataa ccttatgtat catacacata cgatttaggt
      241 gacactatag aataacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca
      301 actgcacctc ggttctatcg attgaattca ccatggaaat ggactacaag gatgacgatg
      361 acaagggtgg tggtggtggt gcgtcgacaa tggctaacat gaacagtgac tcaagacatc
      421 tgggaacaag cgaggtggac cacgagcggg accctggccc tatgaacatt cagtttgagc
      481 cctccgacct gcggtctaag agacccttct gcatcgaacc taccaacatt gtgaacgtca
      541 atcacgtgat ccagagggtc agtgatcatg catcagccat gaataagcga attcactacg
      601 cctcccggct gaccacaccc gctgacaaag cactgatcgc ccctgatcat gtggtcccag
      661 cacccgagga atgttacgtg tatagccctc tgggatccgc ctacaagctg cagagctaca
      721 ccgaggggta tggaaaaaac acatccctgg tgactatttt tatgatctgg aataccatga
      781 tggggacaag tattctgtca atcccatggg gaatcaagca ggccgggttc actaccggaa
      841 tgtgcgtgat cattctgatg ggcctgctga ccctgtactg ctgttatagg gtggtcaaat
      901 ctcgcacaat gatgttttca ctggacacaa ctagctggga gtaccccgat gtgtgtcggc
      961 actattttgg aagcttcggc cagtg
//
