LOCUS       sequence-135258-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral stable expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Wang S, Tsun ZY, Wolfson RL, Shen K, Wyant GA, Plovanich ME, Yuan 
            ED, Jones TD, Chantranupong L, Comb W, Wang T, Bar-Peled L, Zoncu R,
            Straub C, Kim C, Park J, Sabatini BL, Sabatini DM
  TITLE     Metabolism. Lysosomal amino acid transporter SLC38A9 signals 
            arginine sufficiency to mTORC1.
  JOURNAL   Science. 2015 Jan 9;347(6218):188-94. doi: 10.1126/science.1257132. 
            Epub 2015 Jan 7.
  PUBMED    25567906
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1257132"; journalName: "Science"; date: "2015-01-9-
            9"; volume: "347"; issue: "6218"; pages: "188-94"
COMMENT     Sequence Label: pLJM60-FLAG-SLC38A9.1 L74A
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        221..239
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     221..238
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     252..271
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             330..353
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 accgtcagat cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg
       61 accgatccag cctccgcggc cgggaacggt gcattggaac gcggattccc cgtgccaaga
      121 gtgacgtaag taccgcctat agagtctata ggcccacccc cttggcttcg ttagaacgcg
      181 gctacaatta atacataacc ttatgtatca tacacatacg atttaggtga cactatagaa
      241 taacatccac tttgcctttc tctccacagg tgtccactcc caggtccaac tgcacctcgg
      301 ttctatcgat tgaattcacc atggaaatgg actacaagga tgacgatgac aagggtggtg
      361 gtggtggtgc gtcgacaatg gctaacatga acagtgactc aagacatctg ggaacaagcg
      421 aggtggacca cgagcgggac cctggcccta tgaacattca gtttgagccc tccgacctgc
      481 ggtctaagag acccttctgc atcgaaccta ccaacattgt gaacgtcaat cacgtgatcc
      541 agagggtcag tgatcatgca tcagccatga ataagcgaat tcactactat tcccgggcca
      601 ccacacccgc tgacaaagca ctgatcgccc ctgatcatgt ggtcccagca cccgaggaat
      661 gttacgtgta tagccctctg ggatccgcct acaagctgca gagctacacc gaggggtatg
      721 gaaaaaacac atccctggtg actattttta tgatctggaa taccatgatg gggacaagta
      781 ttctgtcaat cccatgggga atcaagcagg ccgggttcac taccggaatg tgcgtgatca
      841 ttctgatggg cctgctgacc ctgtactgct gtta
//
