LOCUS       sequence-135262-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral stable expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Wang S, Tsun ZY, Wolfson RL, Shen K, Wyant GA, Plovanich ME, Yuan 
            ED, Jones TD, Chantranupong L, Comb W, Wang T, Bar-Peled L, Zoncu R,
            Straub C, Kim C, Park J, Sabatini BL, Sabatini DM
  TITLE     Metabolism. Lysosomal amino acid transporter SLC38A9 signals 
            arginine sufficiency to mTORC1.
  JOURNAL   Science. 2015 Jan 9;347(6218):188-94. doi: 10.1126/science.1257132. 
            Epub 2015 Jan 7.
  PUBMED    25567906
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1257132"; journalName: "Science"; date: "2015-01-9-
            9"; volume: "347"; issue: "6218"; pages: "188-94"
COMMENT     Sequence Label: pLJM60-FLAG-SLC38A9.1 (1-119aa)
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(383..406)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(465..484)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        complement(497..515)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(498..515)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(725..749)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(746..845)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(775..795)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 aggatgtgtt ttttccatac ccctcggtgt agctctgcag cttgtaggcg gatcccagag
       61 ggctatacac gtaacattcc tcgggtgctg ggaccacatg atcaggggcg atcagtgctt
      121 tgtcagcggg tgtggtcagc cgggaatagt agtgaattcg cttattcatg gctgatgcat
      181 gatcactgac cctctggatc acgtgattga cgttcacaat gttggtaggt tcgatgcaga
      241 agggtctctt agaccgcagg tcggagggct caaactgaat gttcataggg ccagggtccc
      301 gctcgtggtc cacctcgctt gttcccagat gtcttgagtc actgttcatg ttagccattg
      361 tcgacgcacc accaccacca cccttgtcat cgtcatcctt gtagtccatt tccatggtga
      421 attcaatcga tagaaccgag gtgcagttgg acctgggagt ggacacctgt ggagagaaag
      481 gcaaagtgga tgttattcta tagtgtcacc taaatcgtat gtgtatgata cataaggtta
      541 tgtattaatt gtagccgcgt tctaacgaag ccaagggggt gggcctatag actctatagg
      601 cggtacttac gtcactcttg gcacggggaa tccgcgttcc aatgcaccgt tcccggccgc
      661 ggaggctgga tcggtcccgg tgtcttctat ggaggtcaaa acagcgtgga tggcgtctcc
      721 aggcgatctg acggttcact aaacgagctc tgcttatata gacctcccac cgtacacgcc
      781 taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag tcccgttgat
      841 tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga aatccccgtg
      901 agtcaaaccg ctatccacgc
//
