LOCUS       sequence-135267-        8773 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.22 protein, C-terminally fused to the Fc region of 
            human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8773)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 8773)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.22 EC10-Fc-His
FEATURES             Location/Qualifiers
     source          1..8773
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             5353..5370
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5396..5517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5433..5452)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5487..5506
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5555..5572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5726..6314)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5806..5825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6485..7276,7277..7345))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7108..7127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7346..7450)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7518..7536
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7574..7596)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7598..7618)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7603..7933
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7784..7919
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7846..7865
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8070..8089
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8156..8611)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8293..8314)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8505..8524
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8737..8759
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8751..8768
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8752..8768
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgctactgcc aacttcacac
     1621 aacccgctca gcggaaacac ggcatacgaa atgaatatgc ccaacgaacg cctcaaatgg
     1681 ctaatgctgt tcgcagctgt tgccctcatt gcttgtggta gtcagaccct agctgccaat
     1741 cccccagatg ccgaccaaaa aggacccgtc ttcctcaagg aacccaccaa ccgcattgac
     1801 ttctccaact ccacgggcgc agagatcgag tgcaaggcca gcggcaatcc catgcccgag
     1861 attatttgga tcaggagcga cggtaccgcc gtgggtgatg tgcccggatt gcgtcagatc
     1921 tcatccgacg gcaagctggt cttccctccc ttccgcgccg aggactaccg ccaggaggtc
     1981 catgcccagg tgtacgcctg cctggcccgc aaccagttcg gatccattat ctcccgggac
     2041 gtccatgtgc gagccgttgt caatcagttc tatgaggcgg agattatgac cgagtatgtt
     2101 atcaggggta atgctgccgt tctgaagtgt tccatcccgt cgttcgtggc tgattttgtt
     2161 cgagttgagt cctggatcga tgatgagggc aatgtcttgt ccttttcgga caattacgat
     2221 ggaaagtacc tggtattgcc ctctggagag ctgcacatcc gtgaggttgg acccgaggac
     2281 ggatacaaga gctaccagtg ccgaaccaaa catcgtctga ccggagaaac ccgattaagt
     2341 gccacaaaag gacgattggt catcacagaa cctgtgggca gtgttgcgcc taagatcagc
     2401 gtagaaaatc gtctgaaaaa tgcagaggct cgaattggtt ccaccttcac cctcttttgc
     2461 cctggtcaag cctatccagt acccttcttt agatggtaca agttcatcga aggaacaacc
     2521 cgtaaacaag ctgtggtgct aaatgaccgt gtgaagcagg tctctggtac tctcattatt
     2581 aaggacgcag tagtcgagga ctccggaaaa tacctgtgcg tggtgaacaa ttccgtggga
     2641 ggcgaaagcg ttgagactgt gcttaccgtc actgcccccc tatccgctaa gatcgatcct
     2701 cccacacaga cagttgactt cggacgcccc gccgtattca cttgccagta cactggaaat
     2761 cccatcaaga ccgttagctg gatgaaggac ggcaaggcta tcggacacag cgaacccgtg
     2821 ctgcgcatcg aatccgtgaa gaaggaggac aagggcatgt accagtgctt cgtaagaaac
     2881 gaccaggaat cggcggaggc gagtgctgag ctgaagctcg gaggccgttt cgatcccccc
     2941 gtcatccgac aggccttcca ggaagagacc atggaacccg gaccaagtgt attcctcaag
     3001 tgcgtcgccg gcggaaaccc cacgcccgaa atttcctggg aactcgacgg caagaaaatc
     3061 gccaacaacg atcgctatca ggtcggacag tatgtgacgg tcaacggaga tgtggtttcc
     3121 tatctgaaca taacctcggt ccacgccaac gatggcggtc tctacaagtg catagccaag
     3181 agcaaagtgg gcgtggccga acactcggcc aagttgaatg tatatggact gccctacatc
     3241 cggcaaatgg agaagaaggc aattgttgct ggagaaaccc tgattgtcac atgtcctgta
     3301 gctggatacc ccatcgattc gattgtctgg gagcgagata accgtgccct gcccatcaac
     3361 cgcaaacaga aggtcttccc caacggaacg ctgattatcg agaatgtgga acggaactca
     3421 gaccaagcga cctacacttg cgttgccaag aatcaggaag gatactctgc tcgaggatct
     3481 ctggaagtgc aagtcatggt tcccccgaaa atcacacctt tcacctttgg cgaggatccc
     3541 accaatgtgg aggactcagt ctctgtgact tgtttgatat ccagtggaga cttacccatc
     3601 gatatcgagt ggctatttaa tgacttcgga atcagctcgt attcaggaat gactgtcctc
     3661 agaggtggaa agagaaccag catgctgacc atagataatg tccatgctcg tcatgccgga
     3721 aagtatagtt gtcgcgcaaa gaaccacgca gcagccgtca actacactac tgagttgatt
     3781 gtgaatgtac ctcccagatg gatcctcgag cccaccgata aggccttcgc ccagggatcc
     3841 gatgccaagg ttgaatgcaa ggctgatggc ttccccaaac cccaagttac atggaagaaa
     3901 gcagttggcg acacccccgg agagtacaag gatctaaaga agagcgacaa catccgcgtg
     3961 gaagagggca ctctgcatgt cgacaacatc caaaagacca acgagggcta ctatctgtgc
     4021 gaggctatca atggaatagg ctccggcctc tcggctgtga ttatgatcag cgttcaggcc
     4081 cctccagagt tcacggagaa gctgcgcaac cagaccgccc gacgaggaga acccgccgta
     4141 ctccagtgcg aggcgaaggg cgagaagccc attggcatct tgtggaacat gaacaacatg
     4201 cgactggacc caaagaacga caatcggtac accattcgtg aggagatcct ttccaccgga
     4261 gttatgtcca gtctgtcgat caagcgcacc gagagatccg actccgccct attcacctgt
     4321 gtcgccacta atgcctttgg atccgatgac gccagcataa atatgattgt ccaggaagtt
     4381 ccggagatgc catatgcttt gaaggtactc gacaaatccg gacgttccgt gcagctgagc
     4441 tgggcgcaac cctacgatgg caactcccct ctggacaggt acatcattga gtttaagcgt
     4501 tctcgtgcct cctggagcga aattgatcgc gtcatcgtgc ccggacacac cacagaagcc
     4561 caagtgcaga agctcagccc cgccaccacc tacaacattc gcatcgtggc cgaaaacgcc
     4621 atcggcacat cccaatcctc tgaagcggtc actatcatca ctagtgacaa aactcacaca
     4681 tgcccaccgt gcccagcacc tgaactcctg gggggaccgt cagtcttcct cttcccccca
     4741 aaacccaagg acaccctcat gatctcccgg acccctgagg tcacatgcgt ggtggtggac
     4801 gtgagccacg aagaccctga ggtcaagttc aactggtacg tggacggcgt ggaggtgcat
     4861 aatgccaaga caaagccgcg ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc
     4921 ctcaccgtcc tgcaccagga ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac
     4981 aaagccctcc cagcccccat cgagaaaacc atctccaaag ccaaagggca gccccgagaa
     5041 ccacaggtgt ataccctgcc cccatcccgg gatgagctga ccaagaacca ggtcagcctg
     5101 acctgcctgg tcaaaggctt ctatcccagc gacatcgccg tggagtggga gagcaatggg
     5161 cagccggaga acaactacaa gaccacgcct cccgtgctgg actccgacgg ctccttcttc
     5221 ctctacagca agctcaccgt ggacaagagc aggtggcagc aggggaacgt cttctcatgc
     5281 tccgtgatgc atgaggctct gcacaaccac tacacgcaga agagcctctc cctgtctccg
     5341 ggtaaaggcg gccatcatca ccatcaccat taatctagag atcttattaa agcagaactt
     5401 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     5461 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     5521 tgtctggtcg actctagcta gactcttccg cttcctcgct cactgactcg ctgcgctcgg
     5581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7441 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     7501 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     7561 atgacggtga aaacctctga cacatgcagc tcccggagga atgtgtgtca gttagggtgt
     7621 ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc
     7681 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     7741 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     7801 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     7861 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     7921 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcgtcccgg agacggtcac
     7981 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     8041 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     8101 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     8161 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     8221 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     8281 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     8341 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     8401 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     8461 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     8521 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     8581 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     8641 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     8701 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     8761 cggccagtga att
//
