LOCUS       sequence-135278-        9203 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses 3*FLAG tagged human NSD3-short with 100-263 aa deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 9203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: MSCV-NSD3-short del(100-263)
FEATURES             Location/Qualifiers
     source          1..9203
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1422..1442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2901..2966
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        2983..3482
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3018..3041)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3415..3434
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3503..4102
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3503..3522)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3999..4019
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4163..4730
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4180..4732
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4324..4341)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4551..4570
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4744..4746,4747..4749,4750..5463)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4789..4810)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5050..5069)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5397..5418
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5565..6079
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6248..6264)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6248..6264)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6261..6283)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6272..6288
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6296..6302,6303..6320,6321..6326))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6341..6362
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6479..6496)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6650..7238)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6730..6749)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7409..8200,8201..8269))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8032..8051
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8270..8374)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8442..8460
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8498..8520)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8620..8639
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8833..8855
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8983..9002
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9202..9203,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctaccat gccgaaaaag aagcgcaagg
     1441 taggaggcat ggatttctct ttctctttca tgcaagggat catgggaaac acaattcagc
     1501 aaccacctca actcattgac tccgccaaca tccgtcagga ggatgccttt gataacaaca
     1561 gtgacattgc tgaagatggt ggccagacac catatgaagc tactttgcag caaggctttc
     1621 agtacccagc tacaacagaa gatcttcctc cactcacaaa tgggtatcca tcatcaatca
     1681 gtgtgtatga aactcaaacc aaataccagt catataatca gtatcctaat gggtcagcca
     1741 atggctttac tggtgttaag tttcaggttg gcgatcttgt gtggtccaag gtgggaacct
     1801 atccttggtg gccttgtatg gtttcaagtg atccccagct tgaggttcat actaaaatta
     1861 acacaagagg tgcccgagaa tatcatgtcc agttttttag caaccagcca gagagggcgt
     1921 gggttcatga aaaacgggta cgagagtata aaggtcataa acagtatgaa gaattactgg
     1981 ctgaggcaac caaacaagcc agcaatcact ctgagaaaca aaagattcgg aaaccccgac
     2041 ctcagagaga acgtgctcag tgggatattg gcattgccca tgcagagaaa gcattgaaaa
     2101 tgactcgaga agaaagaata gaacagtata cttttattta cattgataaa cagcctgaag
     2161 aggctttatc ccaagcaaaa aagagtgttg cctccaaaac cgaagttaaa aaaacccgac
     2221 gaccaagatc tgtgctgaat actcagccag aacagaccaa tgcaggggag gtggcctcct
     2281 cactctcaag tactgaaatt cggagacata gccagaggcg gcacacaagt gcggaagagg
     2341 aagagccacc gcctgttaaa atagcctgga aaactgcggc agcaaggaaa tccttaccag
     2401 cttccattac gatgcacaaa gggagcctgg atttgcagaa gtgtaacatg tctccagttg
     2461 tgaaaattga acaagtgttt gctcttcaga atgctacagg ggatgggaaa tttatcgatc
     2521 aatttgttta ttcaacaaag ggaattggta acaaaacaga aataagtgtc agggggcaag
     2581 acaggcttat aatttctaca ccaaaccaga gaaatgaaaa gccaacgcag agtgtatcat
     2641 ctcctgaagc aacatctggt tctacaggct cagtagaaaa gaagcaacag agaagatcaa
     2701 ttagaactcg ttctgaatca gagaaatcca ctgaggttgt gccaaagaag aagatcaaaa
     2761 aggagcaggt tgaaacagtt cctcaggcta cagtgaagac tggattacag aaagggtcgg
     2821 cggaccgggg agtgcagggc tctgtcagat tcagtgacag ctccgtctcc gcagcgattg
     2881 aggaaactgt ggacggaggc gattacaaag accatgacgg tgattataaa gatcatgata
     2941 tcgattacaa ggatgacgat gacaagtgat gagaattcta ccgggtaggg gaggcgcttt
     3001 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     3061 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     3121 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     3181 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     3241 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     3301 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     3361 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     3421 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     3481 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     3541 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     3601 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     3661 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     3721 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     3781 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     3841 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     3901 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     3961 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     4021 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     4081 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     4141 cgcacgaccc catgcatcca attccgcccc ccccccctaa cgttactggc cgaagccgct
     4201 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     4261 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     4321 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     4381 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     4441 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     4501 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     4561 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     4621 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aaacgtctag
     4681 gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca
     4741 accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg
     4801 gacggcgacg taaacggcca caagttcagc gtgtctggcg agggcgaggg cgatgccacc
     4861 tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc
     4921 accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg
     4981 aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc
     5041 ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc
     5101 ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg
     5161 cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga caagcagaag
     5221 aacggcatca aggcgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc
     5281 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     5341 cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg
     5401 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     5461 taatgaatta attaagaatt atcaagctta tcgataccgt cgacctgcag ccaagcttat
     5521 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     5581 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact
     5641 gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac
     5701 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     5761 atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     5821 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     5881 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     5941 agtcctccga tagactgcgt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     6001 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     6061 gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac attctgaggg
     6121 taggagtcga atattaagta atcctgactc aattagccac tgttttgaat ccacatactc
     6181 caatactcct gaaatagttc attatggaca gcgcagaaga gctggggaga attaattcgt
     6241 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6301 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6361 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6421 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6481 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6541 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6601 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6661 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6721 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6781 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6841 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6901 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6961 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7021 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7081 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7141 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7201 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7261 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7321 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7381 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7441 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7501 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7561 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7621 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7681 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7741 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7801 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7861 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     7921 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     7981 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8041 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8101 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8161 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8221 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8281 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8341 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8401 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     8461 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     8521 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     8581 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     8641 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     8701 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8761 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     8821 cgccagggtt ttcccagtca cgacgttgta aaacgacggc gcaaggaatg gtgcatgcaa
     8881 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
     8941 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
     9001 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
     9061 gcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca
     9121 acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt
     9181 tagtctccag aaaaaggggg gaa
//
