LOCUS       sequence-135282-        1112 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses 3*FLAG tagged human NSD3-short with W284A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 1112)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: MSCV-NSD3-short W284A
FEATURES             Location/Qualifiers
     source          1..1112
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..25
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     CDS             complement(77..142)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc
       61 ggtagaattc tcatcacttg tcatcgtcat ccttgtaatc gatatcatga tctttataat
      121 caccgtcatg gtctttgtaa tcgcctccgt ccacagtttc ctcaatcgct gcggagacgg
      181 agctgtcact gaatctgaca gagccctgca ctccccggtc cgccgaccct ttctgtaatc
      241 cagtcttcac tgtagcctga ggaactgttt caacctgctc ctttttgatc ttcttctttg
      301 gcacaacctc agtggatttc tctgattcag aacgagttct aattgatctt ctctgttgct
      361 tcttttctac tgagcctgta gaaccagatg ttgcttcagg agatgataca ctctgcgttg
      421 gcttttcatt tctctggttt ggtgtagaaa ttataagcct gtcttgcccc ctgacactta
      481 tttctgtttt gttaccaatt ccctttgttg aataaacaaa ttgatcgata aatttcccat
      541 cccctgtagc attctgaaga gcaaacactt gttcaatttt cacaactgga gacatgttac
      601 acttctgcaa atccaggctc cctttgtgca tcgtaatgga agctggtaag gatttccttg
      661 ctgccgcagt tttccaggct attttaacag gcggtggctc ttcctcttcc gcacttgtgt
      721 gccgcctctg gctatgtctc cgaatttcag tacttgagag tgaggaggcc acctcccctg
      781 cattggtctg ttctggctga gtattcagca cagatcttgg tcgtcgggtt tttttaactt
      841 cggttttgga ggcaacactc ttttttgctt gggataaagc ctcttcaggc tgtttatcaa
      901 tgtaaataaa agtatactgt tctattcttt cttctcgagt cattttcaat gctttctctg
      961 catgggcaat gccaatatcc cactgagcac gttctctctg aggtcggggt ttccgaatct
     1021 tttgtttctc agagtgattg ctggcttgtt tggttgcctc agccagtaat tcttcatact
     1081 gtttatgacc tttatactct cgtacccgtt tt
//
