LOCUS       sequence-135283-        9662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses 3*FLAG tagged human NSD3-short with W284A mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Shen C, Ipsaro JJ, Shi J, Milazzo JP, Wang E, Roe JS, Suzuki Y, 
            Pappin DJ, Joshua-Tor L, Vakoc CR
  TITLE     NSD3-Short Is an Adaptor Protein that Couples BRD4 to the CHD8 
            Chromatin Remodeler.
  JOURNAL   Mol Cell. 2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033.
  PUBMED    26626481
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Nov 24. pii: S1097-2765(15)00824-2. doi: 
            10.1016/j.molcel.2015.10.033."
COMMENT     Sequence Label: MSCV-NSD3-short W284A
FEATURES             Location/Qualifiers
     source          1..9662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             3360..3425
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     promoter        3442..3941
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3477..3500)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3874..3893
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3962..4561
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3962..3981)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4458..4478
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4622..5189
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4639..5191
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4783..4800)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5010..5029
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5203..5205,5206..5208,5209..5922)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5248..5269)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5509..5528)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5856..5877
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             6024..6538
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6707..6723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6707..6723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6720..6742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6731..6747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6755..6761,6762..6779,6780..6785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6800..6821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6938..6955)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7109..7697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7189..7208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7868..8659,8660..8728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8491..8510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8729..8833)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8901..8919
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8957..8979)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9079..9098
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9292..9314
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9442..9461
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9661..9662,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatccaccat ggatttctct ttctctttca
     1441 tgcaagggat catgggaaac acaattcagc aaccacctca actcattgac tccgccaaca
     1501 tccgtcagga ggatgccttt gataacaaca gtgacattgc tgaagatggt ggccagacac
     1561 catatgaagc tactttgcag caaggctttc agtacccagc tacaacagaa gatcttcctc
     1621 cactcacaaa tgggtatcca tcatcaatca gtgtgtatga aactcaaacc aaataccagt
     1681 catataatca gtatcctaat gggtcagcca atggctttgg tgcagttaga aactttagcc
     1741 ccactgacta ttatcattca gaaattccaa acacaagacc acatgaaatt ctggaaaaac
     1801 cttcccctcc acagccacca cctcctcctt cggtaccaca aactgtgatt ccaaagaaga
     1861 ctggctcacc tgaaattaaa ctaaaaataa ccaaaactat ccagaatggc agggaattgt
     1921 ttgagtcttc cctttgtgga gaccttttaa atgaagtaca ggcaagtgag cacacgaaat
     1981 caaagcatga aagcagaaaa gaaaagagga aaaaaagcaa caagcatgac tcatcaagat
     2041 ctgaagagcg caagtcacac aaaatcccca aattagaacc agaggaacaa aatagaccaa
     2101 atgagagggt tgacactgta tcagaaaaac caagggaaga accagtacta aaagaggaag
     2161 ccccagttca gccaatacta tcttctgttc caacaacgga agtgtccact ggtgttaagt
     2221 ttcaggttgg cgatcttgtg tggtccaagg tgggaaccta tccttgggcg ccttgtatgg
     2281 tttcaagtga tccccagctt gaggttcata ctaaaattaa cacaagaggt gcccgagaat
     2341 atcatgtcca gttttttagc aaccagccag agagggcgtg ggttcatgaa aaacgggtac
     2401 gagagtataa aggtcataaa cagtatgaag aattactggc tgaggcaacc aaacaagcca
     2461 gcaatcactc tgagaaacaa aagattcgga aaccccgacc tcagagagaa cgtgctcagt
     2521 gggatattgg cattgcccat gcagagaaag cattgaaaat gactcgagaa gaaagaatag
     2581 aacagtatac ttttatttac attgataaac agcctgaaga ggctttatcc caagcaaaaa
     2641 agagtgttgc ctccaaaacc gaagttaaaa aaacccgacg accaagatct gtgctgaata
     2701 ctcagccaga acagaccaat gcaggggagg tggcctcctc actctcaagt actgaaattc
     2761 ggagacatag ccagaggcgg cacacaagtg cggaagagga agagccaccg cctgttaaaa
     2821 tagcctggaa aactgcggca gcaaggaaat ccttaccagc ttccattacg atgcacaaag
     2881 ggagcctgga tttgcagaag tgtaacatgt ctccagttgt gaaaattgaa caagtgtttg
     2941 ctcttcagaa tgctacaggg gatgggaaat ttatcgatca atttgtttat tcaacaaagg
     3001 gaattggtaa caaaacagaa ataagtgtca gggggcaaga caggcttata atttctacac
     3061 caaaccagag aaatgaaaag ccaacgcaga gtgtatcatc tcctgaagca acatctggtt
     3121 ctacaggctc agtagaaaag aagcaacaga gaagatcaat tagaactcgt tctgaatcag
     3181 agaaatccac tgaggttgtg ccaaagaaga agatcaaaaa ggagcaggtt gaaacagttc
     3241 ctcaggctac agtgaagact ggattacaga aagggtcggc ggaccgggga gtgcagggct
     3301 ctgtcagatt cagtgacagc tccgtctccg cagcgattga ggaaactgtg gacggaggcg
     3361 attacaaaga ccatgacggt gattataaag atcatgatat cgattacaag gatgacgatg
     3421 acaagtgatg agaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     3481 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     3541 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     3601 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     3661 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     3721 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     3781 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     3841 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     3901 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac
     3961 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4021 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4081 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4141 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4201 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4261 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4321 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4381 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4441 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4501 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     4561 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatccaa
     4621 ttccgccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     4681 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     4741 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     4801 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     4861 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     4921 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     4981 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     5041 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     5101 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
     5161 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatggtgag caagggcgag
     5221 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     5281 aagttcagcg tgtctggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     5341 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     5401 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     5461 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     5521 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     5581 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     5641 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggcgaacttc
     5701 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     5761 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     5821 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     5881 gccgccggga tcactctcgg catggacgag ctgtacaagt aatgaattaa ttaagaatta
     5941 tcaagcttat cgataccgtc gacctgcagc caagcttatc gataaaataa aagattttat
     6001 ttagtctcca gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt
     6061 aagtaacgcc attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc
     6121 aaggttagga acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt
     6181 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag
     6241 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
     6301 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
     6361 gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc
     6421 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
     6481 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcatgg
     6541 gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa tattaagtaa
     6601 tcctgactca attagccact gttttgaatc cacatactcc aatactcctg aaatagttca
     6661 ttatggacag cgcagaagag ctggggagaa ttaattcgta atcatggtca tagctgtttc
     6721 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     6781 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     6841 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     6901 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     6961 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     7021 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7141 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7621 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7741 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7801 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7861 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7921 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7981 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8041 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8101 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8161 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8221 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8281 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8341 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8401 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8461 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     8521 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8581 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8641 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8701 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8821 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     8881 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg
     8941 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     9001 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     9061 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt
     9121 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
     9181 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     9241 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     9301 gacgttgtaa aacgacggcg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc
     9361 cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg
     9421 gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt
     9481 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa
     9541 agacaggata tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc
     9601 tatagagtac gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg
     9661 aa
//
