LOCUS       sequence-135291-        9113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression plasmid for mouse Erg expression; containing 
            bicistronic IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9113)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 9113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: MSCV PIG Erg
FEATURES             Location/Qualifiers
     source          1..9113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        2893..3392
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2928..2951)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3325..3344
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3413..4012
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3413..3432)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3909..3929
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4073..4640
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4090..4642
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4234..4251)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4461..4480
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4654..4656,4657..4659,4660..5373)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4699..4720)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4960..4979)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5307..5328
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5475..5989
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6158..6174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6158..6174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6171..6193)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6182..6198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6206..6212,6213..6230,6231..6236))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6251..6272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6389..6406)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6560..7148)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6640..6659)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7319..8110,8111..8179))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7942..7961
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8180..8284)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8352..8370
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8408..8430)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8530..8549
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8743..8765
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8893..8912
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9112..9113,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctgccag catgatccag actgtacctg
     1441 acccagcagc tcatattaag gaggccttgt cagttgtgag cgaggaccag tcactatttg
     1501 agtgtgccta cggaacgcca cacctggcta agacagagat gaccgcatcc tcttccagtg
     1561 actatggcca gacatccaag atgagtccca gagtccctca gcaggactgg ctgtctcaag
     1621 ccccagccag ggtcaccatc aagatggagt gcaaccctag tcaggtgaat ggttccagga
     1681 actcacctga tgagtgcagt gtgaacaaag gtgggaagat ggtgggcagc ccggatactg
     1741 tggggatgag ctacggcagc tacatggagg agaagcatgt gccgcctccc aatatgacca
     1801 caaatgagcg cagagtgatc gtccctgcag atcctactct gtggagcaca gaccatgtcc
     1861 gacagtggct ggagtgggcg gtgaaagaat atggcctcct cgatgtggac gtcttactat
     1921 ttcagaatat cgatgggaag gagctgtgca agatgacaaa ggatgacttc cagcggctca
     1981 cgccgagcta caatgccgac attcttctct cacatctcca ctacctcaga gagactcccc
     2041 ttccacatct gacttccgat gacgttgata aggctttaca aaactctcca cggttaatgc
     2101 atgccagaaa cacagggggt gcagctttta ttttcccaaa tacttcagta tatcccgaag
     2161 ctacgcaaag aattacaact aggccagatt taccttatga gcctcccagg agatcagcct
     2221 ggaccggcca cagccacctc acccctcagt ccaaagctgc tcagccatct ccctctgcag
     2281 tgcccaaaac tgaagaccag cgtcctcagt tagatcctta ccagatcctg ggaccgacca
     2341 gtagccgcct tgctaatcca ggtagtggcc agatccagct gtggcagttc ctgctcgaac
     2401 tcctgtcaga cagctccaac tccaactgca tcacctggga aggcaccaac ggggagttca
     2461 agatgacaga cccggacgag gtggctcggc gctgggggga gaggaagagc aagcccaaca
     2521 tgaactatga caagctcagc cgcgccctcc gctactacta cgacaaaaac atcatgacca
     2581 aggtgcacgg gaagcgctac gcctacaagt ttgacttcca cgggattgcc caggccctgc
     2641 agccccaccc tcctgagtcg tccctgtaca agtacccctc cgacctgcca tacatgggct
     2701 cctatcacgc ccacccccag aagatgaact ttgtgtctcc ccaccctccc gctctcccag
     2761 tcacatcttc cagtttcttt gcttccccga acccatactg gaattcaccg actgggggca
     2821 tctacccgaa cactaggctc ccagccagcc atatgccctc tcacctgggc acctactact
     2881 agcaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     2941 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     3001 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     3061 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     3121 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     3181 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     3241 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     3301 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     3361 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     3421 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     3481 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     3541 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     3601 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     3661 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     3721 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     3781 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     3841 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     3901 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     3961 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc
     4021 ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc catgcatcca attccgcccc
     4081 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     4141 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     4201 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     4261 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     4321 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     4381 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     4441 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     4501 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     4561 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     4621 tttgaaaaac acgatgataa tatggccaca accatggtga gcaagggcga ggagctgttc
     4681 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     4741 gtgtctggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     4801 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
     4861 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     4921 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     4981 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5041 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5101 aacgtctata tcatggccga caagcagaag aacggcatca aggcgaactt caagatccgc
     5161 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5221 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc
     5281 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5341 atcactctcg gcatggacga gctgtacaag taatgaatta attaagaatt atcaagctta
     5401 tcgataccgt cgacctgcag ccaagcttat cgataaaata aaagatttta tttagtctcc
     5461 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc
     5521 cattttgcaa ggcatggaaa atacataact gagaatagag aagttcagat caaggttagg
     5581 aacagagaga cagcagaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     5641 ggctcagggc caagaacaga tggtccccag atgcggtccc gccctcagca gtttctagag
     5701 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     5761 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     5821 aagagcccac aacccctcac tcggcgcgcc agtcctccga tagactgcgt cgcccgggta
     5881 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     5941 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatg ggtaacagtt
     6001 tcttgaagtt ggagaacaac attctgaggg taggagtcga atattaagta atcctgactc
     6061 aattagccac tgttttgaat ccacatactc caatactcct gaaatagttc attatggaca
     6121 gcgcagaaga gctggggaga attaattcgt aatcatggtc atagctgttt cctgtgtgaa
     6181 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     6241 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     6301 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     6361 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6421 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6481 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6541 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6601 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6661 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6721 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6781 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6841 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6901 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6961 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     7021 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     7081 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     7141 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     7201 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     7261 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7321 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7381 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7441 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7501 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7561 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7621 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7681 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7741 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7801 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7861 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7921 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7981 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     8041 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     8101 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8161 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8221 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8281 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     8341 taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     8401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     8461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     8521 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     8581 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
     8641 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
     8701 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
     8761 aaacgacggc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
     8821 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     8881 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     8941 gatgccggcc acgatgcgtc cggcgtagag gcgattagtc caatttgtta aagacaggat
     9001 atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc ctatagagta
     9061 cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg gaa
//
