LOCUS       sequence-135293-        9581 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral expression plasmid for mouse Myb expression; containing 
            bicistronic IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Roe JS, Mercan F, Rivera K, Pappin DJ, Vakoc CR
  TITLE     BET Bromodomain Inhibition Suppresses the Function of Hematopoietic 
            Transcription Factors in Acute Myeloid Leukemia.
  JOURNAL   Mol Cell. 2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011.
  PUBMED    25982114
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 May 12. pii: S1097-2765(15)00267-1. doi: 
            10.1016/j.molcel.2015.04.011."
COMMENT     Sequence Label: MSCV PIG Myb
FEATURES             Location/Qualifiers
     source          1..9581
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        3361..3860
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3396..3419)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     3793..3812
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             3881..4480
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3881..3900)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4377..4397
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4541..5108
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4558..5110
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4702..4719)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4929..4948
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5122..5124,5125..5127,5128..5841)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5167..5188)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5428..5447)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5775..5796
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5943..6457
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6626..6642)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6626..6642)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6639..6661)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6650..6666
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6674..6680,6681..6698,6699..6704))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6719..6740
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6857..6874)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7028..7616)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7108..7127)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7787..8578,8579..8647))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8410..8429
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8648..8752)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8820..8838
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8876..8898)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8998..9017
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9211..9233
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9361..9380
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9580..9581,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctccacc atggcccgga gaccccgaca
     1441 cagcatctac agtagcgatg aagatgatga agacattgag atgtgtgacc atgactacga
     1501 tgggctgctg cccaaatctg gaaagcgtca cttggggaaa actaggtgga caagggaaga
     1561 ggatgagaag ctgaagaagc tggtggaaca gaacggaaca gacgactgga aagtcattgc
     1621 caattatctg cccaaccgga cagatgtgca gtgccaacac cggtggcaga aagtgctgaa
     1681 ccctgaactc atcaaaggtc cctggaccaa agaagaagat cagagagtca tagagcttgt
     1741 ccagaaatat ggtccgaagc gttggtctgt tattgccaag cacttaaaag ggagaattgg
     1801 aaagcagtgt cgggagaggt ggcacaacca tttgaatcca gaagttaaga aaacctcctg
     1861 gacagaagag gaggacagaa tcatttacca ggcacacaag cgtctgggga acagatgggc
     1921 agagatcgca aagctgctgc ccggacggac tgataatgct atcaagaacc actggaattc
     1981 caccatgcgt cgcaaggtgg aacaggaagg ctacctgcag gagccttcca aagccagcca
     2041 gacgccagtg gccacgagct tccagaagaa caatcatttg atggggtttg ggcatgcctc
     2101 acctccatct cagctctctc caagtggcca gtcctccgtc aacagcgaat atccctatta
     2161 ccacatcgcc gaagcacaaa acatctccag tcacgttccc tatcctgtcg cattgcatgt
     2221 taatatagtc aacgtccctc agccggctgc ggcagccatc cagagacact ataacgacga
     2281 agaccctgag aaggaaaagc gaataaagga gctggagttg ctcctgatgt caacagagaa
     2341 cgagctgaag ggacagcagg cattaccaac acagaaccac acttgcagct accccgggtg
     2401 gcacagcacc tccattgtgg accagaccag acctcatggg gatagtgcac ctgtttcctg
     2461 tttgggagaa caccatgcca ccccatctct gcctgcagat cccggctccc tacctgaaga
     2521 aagtgcctca ccagcaaggt gcatgatcgt ccaccagggc accattctgg acaatgttaa
     2581 gaacctctta gaatttgcag aaacactcca gtttatagat tctttcttga acacttccag
     2641 caaccatgaa agctcgggct tagatgcacc taccttaccc tccactcctc tcattggtca
     2701 caaactgaca ccatgtcgag accagactgt gaaaacccag aaggaaaatt ccatctttag
     2761 aactccagct atcaaaaggt caatcctcga aagctctcct cgaactccca caccattcaa
     2821 acatgccctt gcagctcaag aaattaaata cggtcccctg aagatgctac ctcagacccc
     2881 ctcccatgca gtggaggacc tacaagatgt gattaagcag gaatcggatg aatctggaat
     2941 tgtggctgag tttcaagaga gtggaccacc gttactgaaa aaaatcaagc aggaggtgga
     3001 gtcgccaact gagaaatcgg gaaacttctt ctgctcaaac cactgggcag agaacagcct
     3061 gagcacccag ctgttctcgc aggcgtctcc tgtggcagat gccccaaata ttcttacaag
     3121 ctctgtttta atgacacctg tatcagaaga tgaagacaat gtcctcaaag cctttaccgt
     3181 acctaagaac aggcccctgg tgggtccctt gcagccatgc agtggtgcct gggagccagc
     3241 atcctgtggg aagacagagg accagatgac ggcctccggt ccggctcgga aatacgtgaa
     3301 cgcgttctca gctcgaactc tggtcatgtg agacatttct cgaggatcgt gaattctacc
     3361 gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg
     3421 gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc accggtaggc
     3481 gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct cccctagtca
     3541 ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac
     3601 gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt
     3661 ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg ctgggaaggg
     3721 gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct
     3781 ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt
     3841 cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt acaagcccac
     3901 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     3961 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4021 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4081 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4141 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4201 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4261 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4321 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4381 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4441 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc acgacccgca
     4501 gcgcccgacc gaaaggagcg cacgacccca tgcatccaat tccgcccccc ccccctaacg
     4561 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     4621 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     4681 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     4741 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     4801 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     4861 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     4921 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     4981 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     5041 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     5101 gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg
     5161 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtctggcgag
     5221 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     5281 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     5341 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     5401 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     5461 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     5521 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     5581 atggccgaca agcagaagaa cggcatcaag gcgaacttca agatccgcca caacatcgag
     5641 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     5701 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     5761 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     5821 atggacgagc tgtacaagta atgaattaat taagaattat caagcttatc gataccgtcg
     5881 acctgcagcc aagcttatcg ataaaataaa agattttatt tagtctccag aaaaaggggg
     5941 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
     6001 catggaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     6061 gcagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     6121 agaacagatg gtccccagat gcggtcccgc cctcagcagt ttctagagaa ccatcagatg
     6181 tttccagggt gccccaagga cctgaaatga ccctgtgcct tatttgaact aaccaatcag
     6241 ttcgcttctc gcttctgttc gcgcgcttct gctccccgag ctcaataaaa gagcccacaa
     6301 cccctcactc ggcgcgccag tcctccgata gactgcgtcg cccgggtacc cgtgtatcca
     6361 ataaaccctc ttgcagttgc atccgacttg tggtctcgct gttccttggg agggtctcct
     6421 ctgagtgatt gactacccgt cagcgggggt ctttcatggg taacagtttc ttgaagttgg
     6481 agaacaacat tctgagggta ggagtcgaat attaagtaat cctgactcaa ttagccactg
     6541 ttttgaatcc acatactcca atactcctga aatagttcat tatggacagc gcagaagagc
     6601 tggggagaat taattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     6661 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6721 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6781 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6841 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6901 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6961 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     7021 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     7081 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     7141 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     7201 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     7261 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     7321 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     7381 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     7441 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     7501 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     7561 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     7621 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     7681 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     7741 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7801 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7861 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7921 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7981 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     8041 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     8101 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     8161 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     8221 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     8281 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     8341 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     8401 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     8461 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     8521 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     8581 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     8641 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     8701 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     8761 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     8821 ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     8881 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     8941 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     9001 catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     9061 taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag
     9121 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
     9181 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcgc
     9241 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
     9301 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
     9361 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
     9421 gatgcgtccg gcgtagaggc gattagtcca atttgttaaa gacaggatat cagtggtcca
     9481 ggctctagtt ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat
     9541 aaaataaaag attttattta gtctccagaa aaagggggga a
//
