LOCUS       sequence-135298-         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vivo protein protein interaction studies/ expression in mammalian
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  TITLE     pGolt1-mCherry, expression in mammalian cell
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGolt1-mCherry
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      185..194
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(336..354)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gtgaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattct
       61 gcagtcgacg gtaccgcggg cccgggatat gagcctcatc tgcgccttcg ccatcgcctt
      121 catcatcagt gtcatgctca tcgcggccaa ccaggtgctg cgcagtggca tgaagccacc
      181 ggtcgccacc atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
      241 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
      301 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
      361 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
      421 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
      481 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
      541 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
      601 cggccccgta atgca
//
