LOCUS       sequence-135299-         349 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 349)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB328
FEATURES             Location/Qualifiers
     source          1..349
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..170)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(194..240)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(229..249)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 aagtgtaaag cctggggtgc ctaatgcggc ccctcgaggg gccgccatag tgactggata
       61 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      121 atatttatat cattttacgt ttctcgttca gcttttttgt acaaacttgt tgataactct
      181 agagtccccc gtgttctctc caaatgaaat gaacttcctt atatagagga agggtcttgc
      241 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
      301 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcg
//
