LOCUS       sequence-135303-       14049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14049)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 14049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB304
FEATURES             Location/Qualifiers
     source          1..14049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    323..447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(484..489,490..507,508..514)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             568..1227
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(634..653)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1569..1874
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1753..1772
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1915..2039)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2074..3885
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     terminator      3956..4208
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4242..4258)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4242..4258)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4255..4277)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4266..4282
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4290..4296,4297..4314,4315..4320))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4335..4356
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    4441..4616
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(6700..7376)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6743..6763)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    7571..7595
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8116..8907
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9153..9741
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9642..9661
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9895..9912
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9927..10067
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9993..10012)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10153..10175
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10411..10605
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10671..11744)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12173..12802)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgggt ctagagttat caacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      421 taaaacacaa catatccagt cactatggcg gcccctcgag gggccgcatt aggcacccca
      481 ggctttacac tttatgcttc cggctcgtat aatgtgtgga ttttgagtta ggatccgtcg
      541 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      601 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      661 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      721 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      781 gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      841 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      901 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg
      961 gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa tccctgggtg
     1021 agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc
     1081 accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt
     1141 catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac
     1201 tgcgatgagt ggcagggcgg ggcgtaaaga tctggatccg gcttactaaa agccagataa
     1261 cagtatgcgt atttgcgcgc tgatttttgc ggtataagaa tatatactga tatgtatacc
     1321 cgaagtatgt caaaaagagg tatgctatga agcagcgtat tacagtgaca gttgacagcg
     1381 acagctatca gttgctcaag gcatatatga tgtcaatatc tccggtctgg taagcacaac
     1441 catgcagaat gaagcccgtc gtctgcgtgc cgaacgctgg aaagcggaaa atcaggaagg
     1501 gatggctgag gtcgcccggt ttattgaaat gaacggctct tttgctgacg agaacagggg
     1561 ctggtgaaat gcagtttaag gtttacacct ataaaagaga gagccgttat cgtctgtttg
     1621 tggatgtaca gagtgatatt attgacacgc ccgggcgacg gatggtgatc cccctggcca
     1681 gtgcacgtct gctgtcagat aaagtccccc gtgaacttta cccggtggtg catatcgggg
     1741 atgaaagctg gcgcatgatg accaccgata tggccagtgt gccggtctcc gttatcgggg
     1801 aagaagtggc tgatctcagc caccgcgaaa atgacatcaa aaacgccatt aacctgatgt
     1861 tctggggaat ataaatgtca ggctccctta tacacagcca gtctgcaggt cgaccatagt
     1921 gactggatat gttgtgtttt acagtattat gtagtctgtt ttttatgcaa aatctaattt
     1981 aatatattga tatttatatc attttacgtt tctcgttcag ctttcttgta caaagtggtt
     2041 cgatctagag gatccccggg tggtcagtcc cttatgttac gtcctgtaga aaccccaacc
     2101 cgtgaaatca aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga
     2161 attgatcagc gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc
     2221 agttttaacg atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat
     2281 cagcgcgaag tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat
     2341 gcggtcactc attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc
     2401 ggctatacgc catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt
     2461 atcaccgttt gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt
     2521 accgacgaaa acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga
     2581 atccatcgca gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg
     2641 gtgacgcatg tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat
     2701 ggtgatgtca gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc
     2761 actagcggga ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc
     2821 tatgaactgt gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc
     2881 ggcatccggt cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac
     2941 tttactggct ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg
     3001 ctgatggtgc acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg
     3061 cattaccctt acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt
     3121 gatgaaactg ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac
     3181 aagccgaaag aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta
     3241 caggcgatta aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt
     3301 attgccaacg aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg
     3361 gaagcaacgc gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc
     3421 gacgctcaca ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac
     3481 ggatggtatg tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt
     3541 ctggcctggc aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg
     3601 ttagccgggc tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg
     3661 ctggatatgt atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg
     3721 aatttcgccg attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg
     3781 atcttcactc gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact
     3841 ggcatgaact tcggtgaaaa accgcagcag ggaggcaaac aatgaatcaa caactctcct
     3901 ggcgcaccat cgtcggctac agcctcggga attgctaccg agctcgaatt tccccgatcg
     3961 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     4021 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     4081 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     4141 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     4201 actagatcgg gaattggttc cggaaccaat tcgtaatcat ggtcatagct gtttcctgtg
     4261 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     4321 gcctggggtg cctaatgagt gagctaactc acattaggct gaattaggcg cgcctatttc
     4381 tgaattcaac acattgcgga cgtttttaat gtactgaatt aacgccgaat taattcgggg
     4441 gatctggatt ttagtactgg attttggttt taggaattag aaattttatt gatagaagta
     4501 ttttacaaat acaaatacat actaagggtt tcttatatgc tcaacacatg agcgaaaccc
     4561 tataggaacc ctaaattccc ttatctggga actactcaca cattattatg gagaaactcg
     4621 aagttagtat atagtccttc catcaccctc tgtgataatc tccttaaaac ttgctccccc
     4681 aggaatcata ggcagaacgt gctcctggtg gggaactatc acatccagta aataaggacc
     4741 gggagaatct agcatagttt gaattgctgc tcttagttca tccttcttca ccacacgcgc
     4801 atttggcacc ttgaaaccgt ctgcaactgt acagaagtcg gggtagatct gctcctcgtg
     4861 atctggatca cctaagtaag tatgtgctct gttggccttg tagaatctat cctccaattg
     4921 tacgaccatt cctagatgtt ggttgtttaa gatcatgatc ttcacgggta acttctccac
     4981 aaaaattgta gccaactcct gtacgttcat cacaaagcta ccatcgccat cgatgtcgat
     5041 cacaggaata tctggttgag ttgcggcagc ccccaaagca gaaggaagtc cgaatcccat
     5101 ggctccaaga cctccagatg tcaaccaacg ccgcggttcg ttgtacttga accactgggc
     5161 tgcccacatt tgatgttggc caacaccagt agatatgatg gcatttccac cagtgagttc
     5221 gtacagcgtc tgtatggcag attgaggagc aatggcatcc tcgaatacgg gaaacttcat
     5281 tggccatttc tgcttttgtt cttctagttc tgccacccag ttggagaagt cgaaagtcct
     5341 cttttgactc ttcaagaggg cattgatttc acttagcgca ggtttcacat cagcacacat
     5401 ggacacatgg ggctgtttgt tctttccaat ttcagcaggg tcaatatcga tatgaacgat
     5461 gctggacctg ctagcaaaag tctccagttt accagtcact cgatcgtcga aacgcactcc
     5521 aaatgccaac aacagatctg cttggtccac ggcgtagttg gcgtaaacag ttccatgcat
     5581 tccaagcatt ttcaaactta atggatcaga ctcgggatac gctcccaaac ccattaaagt
     5641 cgatgtgata ggaattccgg taagctcgac aaattccctc aactcttcgc tggcgtgatt
     5701 gcaacctcct ccagaataca ctacaggctt cttagaacta gaaatgagtc ttataatctg
     5761 ctcgattaac gttggttccg gtggccgagg cagtcgttgt aagtagccct tcagttgcat
     5821 tggctgagac caatctggaa tagccatttg ctgttgcacg tccttgggaa tatctaccaa
     5881 gactgggccc ggcctacctg aagctgccaa aaagaaagcc tccctgatta cgcgagggat
     5941 gtcatcaatg ttcataacaa ggtaattgtg cttggtgatc gatctcgtaa cctccacaat
     6001 tggagtttct tgaaaagcat cggtaccaat catcctactg gatacttgac ctgtaatggc
     6061 cactagtggc acactgtcca agagggcatc agcgagcccg gtaacaagat tggtcgctcc
     6121 aggaccggat gtggctatac aaacccctac tcgtccagac gatttagcat aaccttcagc
     6181 agcgaaaatc tccccctgct cgtggcgaca gagaatgttg cggatgctgc tggaacgtgt
     6241 aagagcctgg tgaatctcca tagacgctcc gccgggataa gcgaacaccg ttctaatccc
     6301 ttctcgctca agagcctcca ccaaaatgtc ggctcctttg cggggctcgt catccttgta
     6361 gcgagactga aagtcggacg gggttggtgc aacgagtgac ttcccgctgc taagccgtgg
     6421 tccactcaca tcgactgcag actggatctt ctcgctaaca gcttgggctt tcacttccag
     6481 tgaacgctca gtcttccgag aggtgttgta tgcatgtccc ctcagagcgg ctcccgagaa
     6541 gaaaccactc ctcaccttta gctctttagc tcccttgcaa caagaccttg ccgatggctg
     6601 caccttctcc aggctcgcta tgctcaaagg cacagctgcg catccactaa ttgacgccat
     6661 gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg
     6721 aaatgaactt ccttatatag aggaagggtc ttgcgaagga tagtgggatt gtgcgtcatc
     6781 ccttacgtca gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc
     6841 ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga
     6901 ggcatcttga acgatagcct ttcctttatc gcaattatgg catttgtagg tgccaccttc
     6961 cttttctact gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt
     7021 tcccgatatt accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc
     7081 tttgatattc ttggagtaga cgagagtgtc gtgctccacc atgttcacat caatccactt
     7141 gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg ggtgggggtc
     7201 catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct ttatcgcaat
     7261 gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag tgacagatag
     7321 ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa gtctcaatag
     7381 ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga gtgtcgtgct
     7441 ccaccatgtt ggcaagctgc tctagccaat acgcaaaccg aattcagaaa taaattcagc
     7501 ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     7561 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     7621 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     7681 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     7741 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     7801 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa
     7861 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     7921 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     7981 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     8041 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     8101 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     8161 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     8221 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     8281 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     8341 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     8401 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     8461 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     8521 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     8581 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     8641 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     8701 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     8761 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     8821 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     8881 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     8941 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     9001 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     9061 ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     9121 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     9181 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     9241 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     9301 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     9361 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     9421 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     9481 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     9541 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     9601 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     9661 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9721 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9781 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9841 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9901 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     9961 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
    10021 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
    10081 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
    10141 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
    10201 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
    10261 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
    10321 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
    10381 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
    10441 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
    10501 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
    10561 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
    10621 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
    10681 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
    10741 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
    10801 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
    10861 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
    10921 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
    10981 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
    11041 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
    11101 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
    11161 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
    11221 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
    11281 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
    11341 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
    11401 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
    11461 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
    11521 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
    11581 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
    11641 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
    11701 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
    11761 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
    11821 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
    11881 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
    11941 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
    12001 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    12061 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    12121 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    12181 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    12241 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    12301 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    12361 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    12421 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    12481 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    12541 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    12601 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    12661 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    12721 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    12781 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    12841 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    12901 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    12961 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    13021 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    13081 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    13141 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    13201 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    13261 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    13321 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    13381 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    13441 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    13501 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    13561 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    13621 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    13681 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    13741 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    13801 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    13861 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    13921 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    13981 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
    14041 gataaacct
//
