LOCUS       sequence-135316-         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway binary vector designed for transgenic research with 
            Marchantia polymorpha as well as other plants.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, 
            Shikanai T, Kohchi T
  TITLE     Development of Gateway Binary Vector Series with Four Different 
            Selection Markers for the Liverwort Marchantia polymorpha.
  JOURNAL   PLoS One. 2015 Sep 25;10(9):e0138876. doi: 
            10.1371/journal.pone.0138876. eCollection 2015.
  PUBMED    26406247
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-09-25"; volume: "10(9):e0138876. doi"; pages: " 
            10.1371/journal.pone.0138876. eCollection 2015"
COMMENT     Sequence Label: pMpGWB223
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(46..170)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(196..213)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(196..212)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(205..227)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    415..439
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 aagtgtaaag cctggggtgc ctaatgcggc ccctcgaggg gccgccatag tgactggata
       61 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
      121 atatttatat cattttacgt ttctcgttca gcttttttgt acaaacttgt tgataactct
      181 agacccaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
      241 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
      301 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gctagagcag
      361 cttgagcttg gatcagattg tcgtttcccg ccttcagttt aaactatcag tgtttgacag
      421 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataatc ggatatttaa
      481 aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt gcatgccaac cacagggttc
      541 ccctcgggat caaagtactt tgatccaacc c
//
