LOCUS       sequence-135337-       10631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variant G6..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10631)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 10631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE
FEATURES             Location/Qualifiers
     source          1..10631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1374..1418
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1471..1518
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    1571..1595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1610..1632
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4682..4726
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4779..4826
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     protein_bind    4879..4903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4918..4940
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5566..5610
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      5663..5710
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5754..6209
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     6051..6072
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(6324..7115,7116..7184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6947..6966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7185..7277)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7358..7946
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7847..7866
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8100..8117
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8132..8274
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8200..8219)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8360..8382
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8376..8567)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(9139..10221)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10183..10202
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10222..10299)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     10505..10524
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 tcggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgttaaat ttagagcatt cagtttcaat tgtcggagct ggaatttctg
      121 ggatagtgtg tgcaattaca ttagctcgtt caagggcatt agaaggttac gcaatagatg
      181 tctatgaaca aagcccccgc gttggtggac ggatccaaac aaacttgttg agcaatcatt
      241 acatcgattt aggtgccgct cgtttttgtt cacaattgca tggtaatgta tatgatctag
      301 tcaagaggtt tgaactcgaa tttgaaccgt ttccttttac tcaacttaga tttactcagc
      361 caattcaaaa agaactcaag actattttgt ctaatctaaa accgttaatc gatgaacata
      421 aagaagattc ttttgtcgaa tttgttacga gctatttggg taaagaaaaa gcaaaagcag
      481 taatcttagc actgggctac gactcactct ctatgccaat aatttctcca catattgctt
      541 acgatatcat tgaaaaacac ccagaaactc aatgtttcac tgaaaattgt gggaatgaat
      601 ggtttagttt gaaagaggga ttggcgtcgt tgatcaaggc gctatatgaa gaggcgttaa
      661 aactcgggat taagtttcat tttgaatcta cattagttca aataagcaat cgaggtaacg
      721 cccacagctt ggtgattgcc gataatcaaa atcaacaaaa agagatcagt aaagggctta
      781 agatcattac tttgcctcca tctcgaatgg aaaaattaga taagcaattt cctaaacgtt
      841 ggagcaacta caattatggt tctagcccct tgtttaaagg atttttcttt tactccgaac
      901 cgtggtggcg agagttaggg ttgacggatc acgtcgtaat tacccaaaat ccgatccgta
      961 aattgtattt caaagatgaa aaatacgcgt ttttctatac tgatagtgac aacgcgttat
     1021 attggcgaga tgtgctttct ttaggcgaag atcattacct tacaacggtt aaaaagctta
     1081 tcgcggaggc actaaactta cctcaagatg tcattccaaa tccagagtta cacaaaaaca
     1141 aattttggct tcatggtgtc gaattttcaa aagaatgttg ccctaaacat ccattttatc
     1201 aaacactttc tgatgacagc gttatctctt tgtctgacgc ctatacacac cattgtggat
     1261 ggatagaagg gggcatctta gccggcaagt ttacagcgga atcgatcatc agccagttgc
     1321 ctacacatcg tgaaactgtg aaagaagtag tatacctttg actcgagtct ggtaaagaaa
     1381 ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagtcgc agcttaatta
     1441 acctaaactg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     1501 gtcttgaggg gttttttgct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
     1561 tcactatttc ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
     1621 ttaagaagga gatatacata tgagtgtttt agattttcct cgaatacatt ttaagggtat
     1681 ggctcgggtt aatataccaa ccgcaaaccg aaatacccaa aacacattgg atacgacaac
     1741 aaataccgtg tttcaattcg gccagccggt tgacattgac ttgccgccgg cacgtttcca
     1801 cgaatacctc cactcacttg gtccaaaagt taatcgcttt ggtaaaaaag atgaaaatgg
     1861 agaattcagt gtagcgtgtg gctacaacat gcttggtaat aatcattttt catggcacga
     1921 cgctaaagtt acggctttgc aattgcgccc gggtgattgt gttgttcaag atccactcat
     1981 agggtgttct gttaatttat ggggacacta caatgagtac cttagaacct cattcaatcg
     2041 tgctcgctgg gttgataatg acccgacccg aagtgactcg gcactcatct atgctggaca
     2101 aattacatta actgcaaatg gcagtgaggc aagtgaaaag catgtgatgt ctgctgacat
     2161 tgattgtaca catggagtga gatggataaa tcctagcttt attaatagcc tcgacgaaca
     2221 tgcgtttgct gaagatattg cagcagcccg tttgtttcag tttgctgtca ataagaacag
     2281 caaggatttc ttattgaatc aattgggctt gcagtcagaa tttattgaac aattacgaat
     2341 agcactagaa caagaagatg ttaagggctt agttgttcaa tattgtgtaa gcaatctatc
     2401 gcctcctgta gcgcccaata tgccggtctt ttgtgattta catggctgca tagggctttg
     2461 gcgagaaagt gaaatggcca cttgtcctgc aggtcggatt ttacagccag aatgcccaca
     2521 gcaatttgca ccgattacat taaaacaggc agggcaatgg ttgagtataa atatgccgat
     2581 tagtatcggt catactcatc acttagatcc attacctgta ttatctggaa tgcctcctcg
     2641 attagcagca aaaacgccct tgggtgtctt gcaactcaag acggccagtg atgagttaat
     2701 cgccgagatc ccagaggtgc tttataacgc gtgtttggat acaggaggga tcatcgatgt
     2761 gacaatacaa acccatacag acggacttga attacagtca ttaaagctgg taagccagtc
     2821 tcattcgtgg catgaaatcg attggcaagt tcaagccgaa caacacatca tatatatgga
     2881 atctccaaac ctaatcgagt tgcaaaaaag caagcaatat attgatgtat atagctattt
     2941 tcgtggaatt cttaagccaa tcaacggttt aacaagttat aacgcgggac cacaacgagt
     3001 gcaatgtgac gagttcattg aaacgaatga acaaggacgt ggtttgtttc tagttgaagc
     3061 gttaacacca gggagtggtg aagtttttct aggagaacaa tatagtcgtg tccagatccg
     3121 tgtattaagt aatgactggg cgttacttaa tgtccctgat gaacaggttg actatgactt
     3181 tctttataaa aatgtcatgg cctactatga gtttgtttat ccgttcatgg cagacaaagt
     3241 gtttagcatg gctgataaat gtaaatgcga aacgtatgca cgcttaatgt ggcaaatgtg
     3301 cgatcccaaa aataaagaga aaagctacta catgcctagt acgcgggaga tgtcacaagc
     3361 acaatcgttt ctatttttga aatatttgag taacgtagaa aagtcggcaa tccccaaggt
     3421 tttgccttat tgcaatacag acgttacggt tacaggcaaa ataaaaaacc gtacacaatt
     3481 aattcgggca ttaaaagact ctgtcgacct agagttgtcg attatgctgc agtatttgta
     3541 tagcgcctat tcactaccaa attacgccgc cggagaggcg ttggtaaacg caaaaagatg
     3601 gactcaagaa caacttgaat tagtgaatgg tggcgcagat aggcggcaca acagcggttg
     3661 gcgaggtgct attcttgaag ttgctcacga agagatgatc cattatttgg tcgtcaataa
     3721 tttgcttatg tccttaggcg aaccttttta ctgtggcaac ccggtacttg gccaacaagc
     3781 gaacgaaaaa tttggactcg atacggattt ttcttacgag ccctttggct tacagtccat
     3841 cacgaaattt gtacgctttg agtggccacg ctttattccg accataggtc tttcaattgc
     3901 tgatttctat gccgacatta agcgtgcctt tgagcaatta ccagacctct atcctaaaga
     3961 tgcgaagggt tcggggggag agcatcactt gtttttgaat gaagtgatta accgtcaata
     4021 cccggcatac caattcgaag tgtataaccg acagactgct ttgtttgcaa ttgaatttgt
     4081 tacagagcaa ggcgaaggtg tgactgcaca gtcagaacaa ttcgaattag gtcatttcca
     4141 ccgcttaaga cagatagcca tggcattaac taatcaagaa cagccttttg aaccggcgta
     4201 tcccgtgctt aaaaaccctg tttttaaacc tagagcggga tgtaatttag tcaccgaccc
     4261 agaatcacaa gccttaatgg cgttttatca acagtgtcat gaattgatgt ttcaaatcat
     4321 gatgcatcat ttttcgcaac gacctctcat cagcatgcgg cgctcgcgat tgatgaatgc
     4381 cgccatcgac ctgatgacag gaatattacg tccattgtct gtgaaattaa tgacgatgcc
     4441 gtctgggact aaaggtcgaa atgccggtcc gccagtgcca gagtcggtca gttctttcgt
     4501 cgataaggat tatgctggcg gttgccaaca attgtcatgg cgctgcgcac agttggcggc
     4561 agcggcaaaa gcgttaaacc taaatgtaac tacacaaatc gaattattgg agtttttcca
     4621 acaacaactg gctgacctcg cagccggaac tctttcaaag gaaggttagc tcgagtctgg
     4681 taaagaaacc gctgctgcga aatttgaacg ccagcacatg gactcgtcta ctagtcgcag
     4741 cttaattaac ctaaactgct gccaccgctg agcaataact agcataaccc cttggggcct
     4801 ctaaacgggt cttgaggggt tttttgctag gatcgagatc gatctcgatc ccgcgaaatt
     4861 aatacgactc actatttcgg aattgtgagc ggataacaat tcccctctag aaataatttt
     4921 gtttaacttt aagaaggaga tatacatatg gaattacgta aagtagatag agttccgcca
     4981 atgttacctg agtgttggag cagtagttat atttcttatt ggcttcctat gaagccagaa
     5041 gacgatatta cttctgggta ttgttggttc gattatgctc aagaggtttg tcgtatagac
     5101 ggtttgttta acccttggag tgaaacaaaa atgggacacc gtttgtggat gtccgaaatt
     5161 atgtatccac gcagttatga atcgtttaaa tctaaaattg cctatgcacg aacacatcgg
     5221 gataaacctt cagcgtttaa agaaacggtg ttaaatgatg aggtagaccc atgccatgag
     5281 cttattttaa cgcgtgatgt gttggtaaaa aaccacgcta agtatgcagg tgaacacacc
     5341 attttgggtt ataaagcgga tgcgtggtgc tttgaacgcc caaatgacaa aggtcctgca
     5401 acgtattttt ttaaagaagg gacagatcag ttgctccgaa tggtcacggg tgatccgaac
     5461 gttcacgcct ctgtaagaga ctttcctaat tttaatacgc tgccgatacc taacacgatt
     5521 tttgatgtaa caagtctctc tcataggaat tgactcgagt ctggtaaaga aaccgctgct
     5581 gcgaaatttg aacgccagca catggactcg tctactagtc gcagcttaat taacctaaac
     5641 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     5701 gggttttttg ctagcgaaag gaggagtcga ctatatccgg attggcgaat gggacgcgcc
     5761 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     5821 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     5881 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     5941 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6001 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6061 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6121 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6181 ttttaacaaa atattaacgt ttacaatttc tggcggcacg atggcatgag attatcaaaa
     6241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7201 tcatgattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     7261 ttagaaaaat aaacaaatag gtcatgacca aaatccctta acgtgagttt tcgttccact
     7321 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7381 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7441 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7501 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7561 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7621 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7681 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7741 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7801 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7861 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     7921 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     7981 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8041 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8101 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     8161 tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc
     8221 atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga
     8281 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     8341 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     8401 aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg
     8461 tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt
     8521 ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg
     8581 taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg
     8641 atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg
     8701 gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt
     8761 aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac
     8821 ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag
     8881 accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc
     8941 tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc
     9001 ctcaacgaca ggagcacgat catgctagtc atgccccgcg cccaccggaa ggagctgact
     9061 gggttgaagg ctctcaaggg catcggtcga gatcccggtg cctaatgagt gagctaactt
     9121 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     9181 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ccagggtggt
     9241 ttttcttttc accagtgaga cgggcaacag ctgattgccc ttcaccgcct ggccctgaga
     9301 gagttgcagc aagcggtcca cgctggtttg ccccagcagg cgaaaatcct gtttgatggt
     9361 ggttaacggc gggatataac atgagctgtc ttcggtatcg tcgtatccca ctaccgagat
     9421 gtccgcacca acgcgcagcc cggactcggt aatggcgcgc attgcgccca gcgccatctg
     9481 atcgttggca accagcatcg cagtgggaac gatgccctca ttcagcattt gcatggtttg
     9541 ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc gctatcggct gaatttgatt
     9601 gcgagtgaga tatttatgcc agccagccag acgcagacgc gccgagacag aacttaatgg
     9661 gcccgctaac agcgcgattt gctggtgacc caatgcgacc agatgctcca cgcccagtcg
     9721 cgtaccgtct tcatgggaga aaataatact gttgatgggt gtctggtcag agacatcaag
     9781 aaataacgcc ggaacattag tgcaggcagc ttccacagca atggcatcct ggtcatccag
     9841 cggatagtta atgatcagcc cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt
     9901 acaggcttcg acgccgcttc gttctaccat cgacaccacc acgctggcac ccagttgatc
     9961 ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg tgcagggcca gactggaggt
    10021 ggcaacgcca atcagcaacg actgtttgcc cgccagttgt tgtgccacgc ggttgggaat
    10081 gtaattcagc tccgccatcg ccgcttccac tttttcccgc gttttcgcag aaacgtggct
    10141 ggcctggttc accacgcggg aaacggtctg ataagagaca ccggcatact ctgcgacatc
    10201 gtataacgtt actggtttca cattcaccac cctgaattga ctctcttccg ggcgctatca
    10261 tgccataccg cgaaaggttt tgcgccattc gatggtgtcc gggatctcga cgctctccct
    10321 tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg
    10381 ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg
    10441 ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc
    10501 catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg
    10561 ccacgatgcg tccggcgtag cctaggatcg agatcgatct cgatcccgcg aaattaatac
    10621 gactcactat t
//
